Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNYC6_RS01815 Genome accession   NZ_CP183416
Coordinates   442442..443533 (-) Length   363 a.a.
NCBI ID   WP_419710811.1    Uniprot ID   -
Organism   Pseudomonas sp. NFX224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 437442..448533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNYC6_RS01790 (ACNYC6_01790) - 437861..438571 (+) 711 WP_419710807.1 histidine phosphatase family protein -
  ACNYC6_RS01795 (ACNYC6_01795) - 438636..438950 (+) 315 WP_019409572.1 SCP2 sterol-binding domain-containing protein -
  ACNYC6_RS01800 (ACNYC6_01800) - 439187..440050 (+) 864 WP_419710808.1 LysR family transcriptional regulator -
  ACNYC6_RS01805 (ACNYC6_01805) - 440157..441302 (+) 1146 WP_419710809.1 sterol desaturase/SRPBCC family protein -
  ACNYC6_RS01810 (ACNYC6_01810) - 441299..442387 (+) 1089 WP_419710810.1 SMP-30/gluconolactonase/LRE family protein -
  ACNYC6_RS01815 (ACNYC6_01815) pilU 442442..443533 (-) 1092 WP_419710811.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNYC6_RS01820 (ACNYC6_01820) - 443647..446184 (+) 2538 WP_419710812.1 PAS domain-containing protein -
  ACNYC6_RS01825 (ACNYC6_01825) - 446254..446529 (+) 276 WP_007933743.1 peptidylprolyl isomerase -
  ACNYC6_RS01830 (ACNYC6_01830) - 446590..447525 (-) 936 WP_419710813.1 sugar kinase -

Sequence

Protein


Download         Length: 363 a.a.        Molecular weight: 40397.22 Da        Isoelectric Point: 6.4441

>NTDB_id=1105642 ACNYC6_RS01815 WP_419710811.1 442442..443533(-) (pilU) [Pseudomonas sp. NFX224]
MEIDALLKRLASQDGSDLYLSTGAPPCARFDGVLAPLTDQPFKPGDIAAIAGSIMDAEQRLEFDRELEMNLAISLAGIGR
FRVNIFKQRNDVSMVVRNVKLDIPRFEDLKLPTVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSRGHIVTIED
PIEYIHRHKQSIINQREVGVDTRSFHAALRNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRGQLLNDLGNNLKACVSQRLVRSRDGQRRVAVEVMLGTPTVADLIRLNQFSELKGVMEKSAAQGMQTFDGAL
YALAVEGVIDEEEALKHADSVNNLRLRLKLHTESTPGDWGLMD

Nucleotide


Download         Length: 1092 bp        

>NTDB_id=1105642 ACNYC6_RS01815 WP_419710811.1 442442..443533(-) (pilU) [Pseudomonas sp. NFX224]
ATGGAAATCGATGCGTTGTTAAAACGCCTGGCCAGCCAGGATGGCTCCGATCTTTACCTTTCCACCGGCGCGCCGCCCTG
TGCGCGTTTCGACGGTGTTCTTGCGCCCCTGACCGATCAGCCGTTCAAACCGGGGGACATCGCGGCGATTGCCGGCTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGATCGTGAACTGGAGATGAACCTGGCCATCTCCCTGGCCGGTATCGGTCGC
TTCCGGGTCAATATTTTCAAACAGCGTAACGATGTGTCGATGGTCGTGCGTAACGTCAAACTCGACATTCCACGTTTCGA
AGACCTCAAGTTGCCGACGGTGTTGCTGGAAACCGTAATGCTCAAGCAAGGCTTGATACTGTTCGTCGGCGCCACCGACT
CGGGCAAGTCTACCTCGCTGGCGGCGCTCATCGACCACCGCAATCGCCATAGTCGCGGCCATATCGTCACCATCGAAGAC
CCGATCGAGTACATCCACCGGCATAAACAATCGATCATCAATCAGCGTGAGGTCGGCGTCGATACGCGCAGCTTTCATGC
CGCGTTGAGAAATACCCTGCGCCAGGCGCCGGACGTGGTGTTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCATG
CCCTGGCCTTCGCCGATACCGGGCATCTGGTGATCTCCACGCTGCATGCTCATAACGCCCATCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGGGCCAATTGCTCAATGATCTGGGGAATAACCTAAAGGCCTGTGTTTCCCAGCG
GCTGGTACGTAGCCGCGATGGGCAACGGCGGGTAGCGGTGGAGGTGATGCTGGGGACGCCGACCGTTGCCGATTTGATCC
GGCTCAATCAGTTTTCCGAGCTCAAGGGTGTGATGGAAAAGTCAGCGGCGCAGGGGATGCAGACGTTTGACGGGGCGCTT
TATGCCTTGGCCGTTGAAGGTGTGATCGATGAGGAAGAGGCGTTGAAACATGCCGATTCGGTGAACAATCTGCGATTGCG
ATTGAAGTTGCACACGGAGTCCACTCCCGGCGATTGGGGGCTCATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.211

97.796

0.54
  pilU Acinetobacter baylyi ADP1

51.124

98.072

0.501
  pilU Vibrio cholerae strain A1552

49.714

96.419

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.003

93.388

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

91.736

0.375
  pilT Vibrio cholerae strain A1552

40.841

91.736

0.375
  pilT Pseudomonas aeruginosa PAK

38.663

94.766

0.366
  pilT Pseudomonas stutzeri DSM 10701

38.663

94.766

0.366
  pilT Legionella pneumophila strain Lp02

39.82

92.011

0.366
  pilT Legionella pneumophila strain ERS1305867

39.82

92.011

0.366
  pilT Neisseria meningitidis 8013

37.971

95.041

0.361

Multiple sequence alignment