Detailed information NTG1104460

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACNT2N_RS15750
• Genome accession: NZ_CP183197
• Coordinates: 3538377..3539495 (+)
• Length: 372 a.a.
• NCBI ID: WP_053121582.1
• Uniprot ID: A0A176NFC4
• Organism: Pseudomonas thivervalensis strain NFX106
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3533377..3544495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACNT2N_RS15735 (ACNT2N_15735)	-	3534318..3535235 (+)	918	WP_053121524.1	sugar kinase	-
ACNT2N_RS15740 (ACNT2N_15740)	-	3535383..3535658 (-)	276	WP_019690952.1	peptidylprolyl isomerase	-
ACNT2N_RS15745 (ACNT2N_15745)	-	3535707..3538244 (-)	2538	WP_208666918.1	hybrid sensor histidine kinase/response regulator	-
ACNT2N_RS15750 (ACNT2N_15750)	pilU	3538377..3539495 (+)	1119	WP_053121582.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACNT2N_RS15755 (ACNT2N_15755)	-	3539660..3541615 (-)	1956	WP_208666917.1	acetoacetate--CoA ligase	-
ACNT2N_RS15760 (ACNT2N_15760)	-	3541811..3542587 (-)	777	WP_053121530.1	3-hydroxybutyrate dehydrogenase	-
ACNT2N_RS15765 (ACNT2N_15765)	-	3542596..3543987 (-)	1392	WP_053121532.1	GntP family permease	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41405.37 Da
• Isoelectric Point: 6.0688

>NTDB_id=1104460 ACNT2N_RS15750 WP_053121582.1 3538377..3539495(+) (pilU) [Pseudomonas thivervalensis strain NFX106]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKPLANEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRAGQRRAAVEVMLGSPTVADLIRRNELGELKSIMEKSEELGMQTFDQAL
FDLVVEGAIDEEEALKNADSANNLRLRLKLHTESGAAAPPSDPAAGEWGLMD

Nucleotide

• Length: 1119 bp

>NTDB_id=1104460 ACNT2N_RS15750 WP_053121582.1 3538377..3539495(+) (pilU) [Pseudomonas thivervalensis strain NFX106]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTCTACATGTCCACGGGCGCACCGCCGTG
CGCGCGTTTCGAGGGGGTGCTCAAACCCTTGGCTAACGAGGCCTTCAAAGTCGGCGACATTGCCCGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATCTCCCTGGCGGGCGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGTAACGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAACTGCCCCGGGTGTTGTTGGACACCATCATGCAGAAACAGGGGCTGATGCTGTTCGTCGGCGCGACTGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAACGGGAAGTCGGTGTCGACACCCGCAGTTTCCACGC
GGCGCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCCTGGCGTTCGCCGACACCGGGCACCTGGTGATTTCCACCTTGCATGCCCATAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTTTTCCCGGAAGAGCGCCGGCCGCAGTTGCTGAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CTTGGTGCGTACCCGCGCGGGTCAGCGGCGGGCGGCGGTGGAGGTCATGCTGGGTTCACCGACGGTGGCCGACCTGATCC
GGCGCAACGAACTCGGTGAGCTCAAGTCCATCATGGAGAAGTCGGAGGAACTGGGGATGCAAACCTTTGACCAGGCGCTG
TTCGACCTGGTGGTCGAAGGCGCCATCGATGAAGAAGAAGCGCTGAAAAATGCCGACTCGGCGAACAACCTGCGATTGCG
TTTGAAGCTGCACACCGAGTCGGGGGCGGCGGCGCCACCGTCTGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

3D structure

• AlphaFold DB: A0A176NFC4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.056	95.43	0.535
pilU	Acinetobacter baylyi ADP1	52.247	95.699	0.5
pilU	Vibrio cholerae strain A1552	52.857	94.086	0.497
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.377	94.892	0.374
pilT	Vibrio cholerae strain A1552	41.141	89.516	0.368
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.516	0.368
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.473	0.366
pilT	Acinetobacter nosocomialis M2	40.419	89.785	0.363
pilT	Acinetobacter baumannii D1279779	40.419	89.785	0.363
pilT	Acinetobacter baumannii strain A118	40.419	89.785	0.363
pilT	Pseudomonas aeruginosa PAK	39.244	92.473	0.363