Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYH_RS18200 Genome accession   NZ_CP180479
Coordinates   3978911..3980020 (-) Length   369 a.a.
NCBI ID   WP_415764381.1    Uniprot ID   -
Organism   Pseudomonas sp. ZB1P45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3973911..3985020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYH_RS18195 - 3974765..3978619 (-) 3855 WP_415764380.1 hypothetical protein -
  ACMXYH_RS18200 pilU 3978911..3980020 (-) 1110 WP_415764381.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYH_RS18205 - 3980141..3982678 (+) 2538 WP_415764382.1 PAS domain S-box protein -
  ACMXYH_RS18210 - 3982744..3983019 (+) 276 WP_007898282.1 peptidylprolyl isomerase -
  ACMXYH_RS18215 - 3983147..3984082 (-) 936 WP_415764383.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41136.08 Da        Isoelectric Point: 6.5089

>NTDB_id=1096037 ACMXYH_RS18200 WP_415764381.1 3978911..3980020(-) (pilU) [Pseudomonas sp. ZB1P45]
MEIDALLRLLASQNGSDLYLSTGAPPSARFEGVLKPLADQPFKPGEVGAIAASIMDAEQRLEFDRELEMNLAISLTGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMIGTPTVGDLIRRNEFSELKGIMEKSSEAGMQTFDGAL
FELFVEGTIDEAEALKHADSLNNLRLRLKMHAEATPGPHTPPGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1096037 ACMXYH_RS18200 WP_415764381.1 3978911..3980020(-) (pilU) [Pseudomonas sp. ZB1P45]
ATGGAAATCGATGCACTGTTAAGGCTACTGGCCAGCCAGAATGGCTCCGATCTTTATCTGTCTACCGGGGCGCCACCTAG
CGCACGGTTCGAGGGTGTGCTCAAGCCCTTGGCCGATCAGCCGTTCAAGCCGGGAGAAGTCGGGGCCATTGCCGCGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGACCGGGAACTGGAGATGAACCTGGCGATCTCTTTGACCGGTGTCGGGCGC
TTTCGAGTGAATATCTTCAAACAACGCAACGATGTGTCGATCGTGGCGCGCAATATCAAACTCGACATTCCGCGTTTCGA
AGACCTCAAACTGCCGCCGGTGCTGCTCGAAACGGTGATGCTCAAACAAGGACTGATGCTGTTCGTCGGCGCCACCGACT
CGGGCAAGTCAACCTCGCTGGCGGCATTGATCGACTACCGCAATCGCCACAGCAGCGGCCATATCATCACGATCGAAGAC
CCGGTCGAGTACATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTTCACGC
CGCATTGAAAAATACCCTGCGCCAGGCACCGGACGTCGTGCTGATCGGCGAAATTCGTGACCGCGAGACGATGGAGCATG
CGCTGGCATTTGCCGATACCGGTCATCTGGTCATTTCCACCCTGCATGCCCACAACGCCCATCAGGCGCTGGACCGCATC
ATCAATTTCTTTCCCGAAGAACGCCGGCCGCAACTCCTGCATGACCTGGGCAATAACCTGAAAGCGTTCGTGTCCCAGCG
CCTGGTGCGTACCCGTGACGGTCAACGACGGGCGGCGGTGGAAGTGATGATTGGCACGCCAACCGTCGGCGACCTGATTC
GGCGCAATGAGTTTTCAGAACTGAAGGGGATCATGGAGAAGTCTTCAGAGGCGGGGATGCAGACCTTCGATGGGGCGCTG
TTCGAGCTGTTTGTCGAGGGTACGATCGATGAGGCAGAAGCGTTGAAACATGCGGATTCATTGAACAACCTGCGCCTGCG
GCTGAAAATGCACGCCGAGGCCACGCCCGGCCCCCATACACCACCGGGTGAATGGGGGCTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.978

99.729

0.558
  pilU Acinetobacter baylyi ADP1

53.09

96.477

0.512
  pilU Vibrio cholerae strain A1552

50

94.851

0.474
  pilT Pseudomonas stutzeri DSM 10701

39.826

93.225

0.371
  pilT Legionella pneumophila strain Lp02

40.058

92.683

0.371
  pilT Legionella pneumophila strain ERS1305867

40.058

92.683

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

90.244

0.369
  pilT Vibrio cholerae strain A1552

40.841

90.244

0.369
  pilT Pseudomonas aeruginosa PAK

39.941

91.599

0.366
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Neisseria gonorrhoeae MS11

39.296

92.412

0.363

Multiple sequence alignment