Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   ACLBK2_RS10975 Genome accession   NZ_CP180236
Coordinates   2091814..2092149 (+) Length   111 a.a.
NCBI ID   WP_048528795.1    Uniprot ID   A0A1J9UM94
Organism   Bacillus sp. Ba 3     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2054745..2092149 2091814..2092149 within 0


Gene organization within MGE regions


Location: 2054745..2092149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLBK2_RS10750 (ACLBK2_10750) - 2054745..2055881 (-) 1137 WP_414835425.1 tyrosine-type recombinase/integrase -
  ACLBK2_RS10755 (ACLBK2_10755) - 2055932..2056420 (-) 489 WP_414835426.1 ImmA/IrrE family metallo-endopeptidase -
  ACLBK2_RS10760 (ACLBK2_10760) - 2056644..2057639 (+) 996 WP_414835427.1 HNH endonuclease -
  ACLBK2_RS10765 (ACLBK2_10765) - 2057673..2058056 (+) 384 WP_414835428.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  ACLBK2_RS10770 (ACLBK2_10770) - 2058213..2059508 (+) 1296 WP_414835429.1 hypothetical protein -
  ACLBK2_RS10775 (ACLBK2_10775) - 2059589..2061169 (+) 1581 WP_414835430.1 serine/threonine protein kinase -
  ACLBK2_RS10780 (ACLBK2_10780) - 2061466..2061867 (+) 402 WP_414835431.1 hypothetical protein -
  ACLBK2_RS10785 (ACLBK2_10785) - 2062061..2062437 (-) 377 Protein_2038 helix-turn-helix domain-containing protein -
  ACLBK2_RS10790 (ACLBK2_10790) - 2062608..2062796 (+) 189 WP_340721012.1 helix-turn-helix transcriptional regulator -
  ACLBK2_RS10795 (ACLBK2_10795) - 2062840..2063139 (+) 300 WP_086392334.1 DNA-binding protein -
  ACLBK2_RS10800 (ACLBK2_10800) - 2063207..2063722 (+) 516 WP_414835432.1 helix-turn-helix domain-containing protein -
  ACLBK2_RS10805 (ACLBK2_10805) - 2063753..2063974 (+) 222 WP_017673355.1 hypothetical protein -
  ACLBK2_RS10810 (ACLBK2_10810) - 2064150..2064434 (+) 285 WP_414835433.1 hypothetical protein -
  ACLBK2_RS10815 (ACLBK2_10815) - 2064553..2065530 (+) 978 WP_414835434.1 DnaD domain-containing protein -
  ACLBK2_RS10820 (ACLBK2_10820) - 2065541..2065903 (+) 363 WP_098481752.1 cell division protein SepF -
  ACLBK2_RS10825 (ACLBK2_10825) - 2065975..2066166 (+) 192 WP_000511363.1 DUF3954 domain-containing protein -
  ACLBK2_RS10830 (ACLBK2_10830) - 2066689..2067075 (+) 387 WP_000360785.1 hypothetical protein -
  ACLBK2_RS10835 (ACLBK2_10835) - 2067940..2068509 (-) 570 WP_001199851.1 cupin domain-containing protein -
  ACLBK2_RS10840 (ACLBK2_10840) - 2068769..2070049 (-) 1281 WP_414835435.1 BclA C-terminal domain-containing protein -
  ACLBK2_RS10845 (ACLBK2_10845) - 2070334..2070594 (+) 261 WP_414835436.1 YopX family protein -
  ACLBK2_RS10850 (ACLBK2_10850) - 2071185..2071460 (+) 276 WP_000873133.1 hypothetical protein -
  ACLBK2_RS10855 (ACLBK2_10855) - 2071582..2071815 (+) 234 WP_000092777.1 hypothetical protein -
  ACLBK2_RS10860 (ACLBK2_10860) - 2072238..2072522 (-) 285 WP_001123250.1 DUF4183 domain-containing protein -
  ACLBK2_RS10865 (ACLBK2_10865) - 2072809..2072940 (+) 132 WP_001061237.1 DUF3983 domain-containing protein -
  ACLBK2_RS10870 (ACLBK2_10870) - 2073045..2073215 (+) 171 WP_414835437.1 hypothetical protein -
  ACLBK2_RS10875 (ACLBK2_10875) - 2073243..2073725 (+) 483 WP_098264261.1 ArpU family phage packaging/lysis transcriptional regulator -
  ACLBK2_RS10880 (ACLBK2_10880) - 2073725..2074267 (+) 543 WP_098277487.1 site-specific integrase -
  ACLBK2_RS10885 (ACLBK2_10885) - 2074481..2075431 (+) 951 WP_414835438.1 nucleoside hydrolase -
  ACLBK2_RS10890 (ACLBK2_10890) - 2076240..2077408 (+) 1169 Protein_2059 serine hydrolase domain-containing protein -
  ACLBK2_RS10895 (ACLBK2_10895) - 2077752..2077970 (+) 219 WP_166689337.1 hypothetical protein -
  ACLBK2_RS10900 (ACLBK2_10900) - 2078416..2078547 (+) 132 Protein_2061 DNA translocase FtsK -
  ACLBK2_RS10905 (ACLBK2_10905) - 2078674..2078880 (-) 207 WP_061139386.1 hypothetical protein -
  ACLBK2_RS10910 (ACLBK2_10910) - 2079195..2080736 (+) 1542 Protein_2063 hypothetical protein -
  ACLBK2_RS10915 (ACLBK2_10915) - 2080749..2081129 (+) 381 WP_166689335.1 DUF1998 domain-containing protein -
  ACLBK2_RS10920 (ACLBK2_10920) - 2081145..2082083 (+) 939 WP_166689333.1 hypothetical protein -
  ACLBK2_RS10925 (ACLBK2_10925) - 2082099..2082416 (+) 318 WP_166689331.1 hypothetical protein -
  ACLBK2_RS10930 (ACLBK2_10930) - 2082486..2082932 (+) 447 WP_166689329.1 phage holin family protein -
  ACLBK2_RS10935 (ACLBK2_10935) - 2083062..2084119 (+) 1058 Protein_2068 N-acetylmuramoyl-L-alanine amidase -
  ACLBK2_RS10940 (ACLBK2_10940) - 2084154..2084462 (-) 309 WP_166689327.1 YolD-like family protein -
  ACLBK2_RS10945 (ACLBK2_10945) - 2084709..2086364 (+) 1656 WP_166689325.1 multicopper oxidase family protein -
  ACLBK2_RS10950 (ACLBK2_10950) - 2086945..2087688 (-) 744 WP_414835439.1 hypothetical protein -
  ACLBK2_RS10955 (ACLBK2_10955) - 2087798..2088445 (+) 648 WP_166689322.1 HD domain-containing protein -
  ACLBK2_RS10960 (ACLBK2_10960) - 2088442..2090337 (+) 1896 WP_166689320.1 ABC-F family ATP-binding cassette domain-containing protein -
  ACLBK2_RS10965 (ACLBK2_10965) - 2090413..2091144 (+) 732 WP_001260655.1 Bax inhibitor-1/YccA family membrane protein -
  ACLBK2_RS10970 (ACLBK2_10970) - 2091250..2091504 (+) 255 WP_048528794.1 DUF4318 domain-containing protein -
  ACLBK2_RS10975 (ACLBK2_10975) ssbA 2091814..2092149 (+) 336 WP_048528795.1 single-stranded DNA-binding protein Machinery gene

Sequence

Protein


Download         Length: 111 a.a.        Molecular weight: 12792.63 Da        Isoelectric Point: 9.0926

>NTDB_id=1094845 ACLBK2_RS10975 WP_048528795.1 2091814..2092149(+) (ssbA) [Bacillus sp. Ba 3]
MMNRVVLIGRLTKEPELYYTKQGVAYARVCVAVNRGFRNSLGEQQVDFINCVIWRRSAENVTEYCTKGSLVGITGRIHTR
NYEDDQGKRIYVTEVVIENITFLEKRREGAS

Nucleotide


Download         Length: 336 bp        

>NTDB_id=1094845 ACLBK2_RS10975 WP_048528795.1 2091814..2092149(+) (ssbA) [Bacillus sp. Ba 3]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAGCAAGGCGTCGCGTATGC
ACGAGTATGTGTTGCTGTGAATAGAGGTTTTCGAAATAGTTTAGGTGAACAACAAGTAGATTTTATTAATTGCGTGATAT
GGAGAAGGTCAGCTGAGAATGTAACTGAATATTGTACGAAAGGTTCACTTGTTGGAATTACCGGACGTATTCATACGAGG
AATTACGAGGATGATCAAGGAAAGAGAATATATGTAACGGAAGTTGTGATTGAAAATATTACATTTTTGGAGAAAAGGCG
GGAGGGTGCATCATAA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9UM94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

59.434

95.495

0.568
  ssb Latilactobacillus sakei subsp. sakei 23K

57.547

95.495

0.55
  ssbB Bacillus subtilis subsp. subtilis str. 168

52.679

100

0.532
  ssbA Streptococcus mutans UA159

44.545

99.099

0.441
  ssbB/cilA Streptococcus pneumoniae TIGR4

44.545

99.099

0.441
  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.545

99.099

0.441
  ssbB/cilA Streptococcus pneumoniae D39

43.636

99.099

0.432
  ssbB/cilA Streptococcus pneumoniae R6

43.636

99.099

0.432
  ssbB/cilA Streptococcus mitis SK321

43.636

99.099

0.432
  ssbB/cilA Streptococcus pneumoniae Rx1

43.636

99.099

0.432
  ssbB Lactococcus lactis subsp. cremoris KW2

44.762

94.595

0.423
  ssbB/cilA Streptococcus mitis NCTC 12261

42.727

99.099

0.423

Multiple sequence alignment