Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACMVO4_RS14130 Genome accession   NZ_CP180205
Coordinates   3065925..3067619 (-) Length   564 a.a.
NCBI ID   WP_414931560.1    Uniprot ID   -
Organism   Vibrio europaeus strain EX1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3060925..3072619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMVO4_RS14110 (ACMVO4_14110) - 3061579..3062406 (-) 828 WP_414931558.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACMVO4_RS14115 (ACMVO4_14115) - 3062500..3064020 (-) 1521 WP_414931559.1 SPOR domain-containing protein -
  ACMVO4_RS14120 (ACMVO4_14120) aroB 3064113..3065201 (-) 1089 WP_171383619.1 3-dehydroquinate synthase -
  ACMVO4_RS14125 (ACMVO4_14125) aroK 3065230..3065748 (-) 519 WP_069667329.1 shikimate kinase AroK -
  ACMVO4_RS14130 (ACMVO4_14130) pilQ 3065925..3067619 (-) 1695 WP_414931560.1 type IV pilus secretin PilQ Machinery gene
  ACMVO4_RS14135 (ACMVO4_14135) pilP 3067633..3068136 (-) 504 WP_414931561.1 pilus assembly protein PilP Machinery gene
  ACMVO4_RS14140 (ACMVO4_14140) pilO 3068126..3068713 (-) 588 WP_414931562.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACMVO4_RS14145 (ACMVO4_14145) pilN 3068700..3069263 (-) 564 WP_171802179.1 PilN domain-containing protein Machinery gene
  ACMVO4_RS14150 (ACMVO4_14150) pilM 3069253..3070248 (-) 996 WP_414931563.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61824.70 Da        Isoelectric Point: 4.6295

>NTDB_id=1094522 ACMVO4_RS14130 WP_414931560.1 3065925..3067619(-) (pilQ) [Vibrio europaeus strain EX1]
MNRIKGMVALARSCLQWFSLFVVLFPSVVYAHEQNDNLLQGLDFKLDEQRNAQIVLTLSDSRAVVDIQKVAQGLSITLPQ
TQVTEALIKVFDVTEFGTAVRDIEVFRHPDKVELIAAINGDFRYHDQLEGTRLQVTVASSSDGQQVDKTSLLDKPGKKLS
LNFQDIPVRNVLQLIADYNDFNLVVSDSVTGNLTLRLEEVSWQKVLDIILQVKGLDKRVEGSIVLVAPKAELDAREQQML
EKARLKQQLGKLSSEIIQVKFAKAADIADLIDAGGEFSMLSPRGSLSVDERTNALLIRDLEQNVDVIRSIVESLDIPVKQ
VQIEARIVTVNEGNLDELGVRWGFSNTNGSTSIGASIEDNVFDVVPIDDMLNVNLGTTSANASTIAFQVAKLGSDMLLDL
ELSALQRESKAEVISSPRLITTNKKPAYIEQGSEIPYLESSSSGATSVSFKKAVLSLKVTPQITPDNRLILDLSVTQDRP
GDVVKTGTGEAVAINTQRIGTQVLVENGETVVLGGIFQHSVTDAVDKVPFFGDLPLLGALFRRTYQQMGKSELLIFVTPK
VVIE

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1094522 ACMVO4_RS14130 WP_414931560.1 3065925..3067619(-) (pilQ) [Vibrio europaeus strain EX1]
ATGAATAGGATAAAAGGAATGGTGGCTTTAGCTAGAAGCTGTTTGCAATGGTTCAGCTTGTTTGTGGTTCTTTTCCCAAG
TGTTGTTTATGCCCATGAGCAAAATGACAACCTCCTGCAAGGCTTGGATTTCAAACTCGATGAACAGCGCAACGCGCAAA
TTGTTCTAACCTTGTCTGACTCTCGTGCTGTGGTTGATATACAAAAAGTGGCGCAGGGGTTAAGTATTACCCTACCTCAA
ACTCAAGTGACGGAAGCGTTAATCAAGGTTTTTGACGTGACCGAGTTTGGCACTGCGGTACGTGACATTGAAGTTTTTCG
CCATCCTGACAAGGTTGAGCTGATAGCGGCAATTAATGGGGACTTTCGTTATCACGATCAACTTGAAGGGACGCGATTGC
AAGTCACCGTGGCGAGCAGTAGCGATGGTCAGCAGGTGGACAAAACGAGTCTTCTCGATAAGCCAGGCAAAAAGTTATCA
CTTAATTTTCAAGATATTCCGGTTAGAAATGTGTTGCAACTAATCGCCGATTACAATGATTTCAACTTGGTTGTTTCCGA
TTCAGTGACTGGCAATCTGACCTTACGCCTTGAAGAGGTATCATGGCAGAAGGTGCTAGATATCATCCTTCAGGTCAAAG
GGTTAGATAAACGAGTCGAAGGAAGCATTGTTTTGGTCGCGCCAAAAGCGGAACTGGATGCTCGTGAGCAGCAGATGCTG
GAGAAAGCGCGTTTAAAGCAACAGTTAGGGAAACTGTCGTCGGAAATTATCCAAGTCAAGTTTGCCAAGGCGGCCGATAT
TGCTGACTTAATCGATGCTGGTGGTGAGTTTAGTATGCTGTCTCCGCGGGGTTCTTTGAGTGTGGACGAGCGCACCAACG
CACTATTGATTCGAGACTTAGAGCAAAATGTCGATGTTATTCGTTCTATTGTTGAATCACTCGATATCCCCGTTAAGCAG
GTGCAAATCGAGGCAAGGATTGTCACGGTTAATGAAGGCAACTTGGATGAGCTTGGGGTACGTTGGGGATTTAGTAACAC
CAATGGCAGTACTTCTATTGGCGCCAGCATTGAAGACAATGTATTCGATGTGGTGCCAATCGATGACATGCTAAACGTGA
ATCTTGGCACAACCTCAGCTAATGCTTCGACCATTGCATTTCAAGTCGCCAAGCTTGGCTCCGACATGTTGCTTGACCTT
GAGCTATCGGCATTACAAAGAGAGTCGAAAGCAGAAGTCATCTCAAGCCCGCGTTTGATCACCACTAATAAAAAGCCAGC
CTATATTGAGCAGGGCAGCGAGATTCCTTATCTAGAGTCATCTTCAAGCGGGGCAACCAGCGTCTCGTTTAAGAAGGCAG
TGCTCAGTTTAAAAGTCACGCCACAGATTACCCCAGATAATCGGCTAATTCTGGATTTGAGCGTGACCCAAGATAGGCCA
GGTGATGTGGTGAAAACCGGTACAGGGGAAGCGGTAGCGATTAACACCCAAAGAATCGGTACCCAAGTGCTGGTGGAGAA
TGGAGAAACCGTGGTTCTCGGGGGCATTTTTCAACACAGCGTGACAGATGCGGTCGACAAAGTGCCTTTCTTTGGTGATT
TGCCTCTGCTAGGGGCGCTATTTCGTCGTACTTACCAACAAATGGGCAAAAGCGAACTGTTAATTTTCGTCACACCAAAG
GTAGTGATTGAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(404-562)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

66.788

97.695

0.652
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

64.386

100

0.651
  pilQ Vibrio cholerae strain A1552

64.386

100

0.651
  pilQ Pseudomonas aeruginosa PAK

41.133

96.986

0.399
  pilQ Legionella pneumophila strain Lp02

41.011

94.681

0.388
  pilQ Legionella pneumophila strain ERS1305867

41.011

94.681

0.388
  comQ Acinetobacter baylyi ADP1

39.209

98.582

0.387
  pilQ Acinetobacter baumannii D1279779

36.503

100

0.381
  pilQ Acinetobacter baumannii strain A118

36.503

100

0.381

Multiple sequence alignment