Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACMVO4_RS14145 Genome accession   NZ_CP180205
Coordinates   3068700..3069263 (-) Length   187 a.a.
NCBI ID   WP_171802179.1    Uniprot ID   -
Organism   Vibrio europaeus strain EX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063700..3074263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMVO4_RS14120 (ACMVO4_14120) aroB 3064113..3065201 (-) 1089 WP_171383619.1 3-dehydroquinate synthase -
  ACMVO4_RS14125 (ACMVO4_14125) aroK 3065230..3065748 (-) 519 WP_069667329.1 shikimate kinase AroK -
  ACMVO4_RS14130 (ACMVO4_14130) pilQ 3065925..3067619 (-) 1695 WP_414931560.1 type IV pilus secretin PilQ Machinery gene
  ACMVO4_RS14135 (ACMVO4_14135) pilP 3067633..3068136 (-) 504 WP_414931561.1 pilus assembly protein PilP Machinery gene
  ACMVO4_RS14140 (ACMVO4_14140) pilO 3068126..3068713 (-) 588 WP_414931562.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACMVO4_RS14145 (ACMVO4_14145) pilN 3068700..3069263 (-) 564 WP_171802179.1 PilN domain-containing protein Machinery gene
  ACMVO4_RS14150 (ACMVO4_14150) pilM 3069253..3070248 (-) 996 WP_414931563.1 type IV pilus assembly protein PilM Machinery gene
  ACMVO4_RS14155 (ACMVO4_14155) - 3070389..3072908 (+) 2520 WP_272279170.1 penicillin-binding protein 1A -
  ACMVO4_RS14160 (ACMVO4_14160) oxyR 3072971..3073864 (-) 894 WP_069667323.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 21537.81 Da        Isoelectric Point: 7.4627

>NTDB_id=1094525 ACMVO4_RS14145 WP_171802179.1 3068700..3069263(-) (pilN) [Vibrio europaeus strain EX1]
MLHNINLLPWREELRVYRQKRFGVLMMVTLIIAVVIQWGIGSYIEQQQERQSERNNYLVRYDTQLDQQLNQLKIVEQEHQ
ALLTRLSVIEDLQKERNKTTDFMNLLPELIPPGVYIDKLKINGLQVELAGISDSTSHLASMLDNLEKSPAITNVQMHSIV
HGTARFASKYQSYRVSFSLLGGQSVSL

Nucleotide


Download         Length: 564 bp        

>NTDB_id=1094525 ACMVO4_RS14145 WP_171802179.1 3068700..3069263(-) (pilN) [Vibrio europaeus strain EX1]
ATGCTGCATAACATCAATTTGTTGCCGTGGCGTGAAGAGCTGCGTGTTTATCGCCAAAAGCGCTTTGGAGTGCTGATGAT
GGTTACGTTAATCATTGCTGTGGTTATCCAGTGGGGCATTGGCAGTTATATAGAGCAGCAACAGGAACGGCAATCTGAAC
GTAATAACTATCTTGTGCGTTATGACACACAGTTGGATCAACAGCTTAACCAACTGAAAATCGTCGAACAAGAACATCAA
GCCCTATTGACCCGCTTGTCGGTTATTGAGGATTTGCAGAAAGAGCGCAATAAAACCACAGACTTTATGAATTTATTGCC
TGAGCTGATTCCGCCTGGTGTTTACATCGACAAGTTAAAGATCAATGGCCTACAGGTTGAGCTTGCTGGGATCAGCGATT
CAACTTCTCATCTTGCCTCTATGCTAGATAACCTCGAAAAATCACCCGCTATAACTAACGTCCAGATGCATTCCATTGTT
CATGGTACGGCTCGATTTGCCAGTAAGTACCAATCATATCGCGTCTCTTTTTCTCTTCTAGGAGGTCAGAGTGTATCGCT
TTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

53.889

96.257

0.519
  pilN Vibrio campbellii strain DS40M4

51.064

100

0.513

Multiple sequence alignment