Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMVO4_RS02490 Genome accession   NZ_CP180205
Coordinates   479571..481262 (-) Length   563 a.a.
NCBI ID   WP_414931889.1    Uniprot ID   -
Organism   Vibrio europaeus strain EX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474571..486262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMVO4_RS02460 (ACMVO4_02460) rplS 475051..475404 (+) 354 WP_004742596.1 50S ribosomal protein L19 -
  ACMVO4_RS02465 (ACMVO4_02465) yacG 475768..475962 (-) 195 WP_069667595.1 DNA gyrase inhibitor YacG -
  ACMVO4_RS02470 (ACMVO4_02470) zapD 476025..476765 (-) 741 WP_004742598.1 cell division protein ZapD -
  ACMVO4_RS02475 (ACMVO4_02475) coaE 476800..477408 (-) 609 WP_171382771.1 dephospho-CoA kinase -
  ACMVO4_RS02480 (ACMVO4_02480) pilD 477410..478279 (-) 870 WP_069667593.1 prepilin peptidase Machinery gene
  ACMVO4_RS02485 (ACMVO4_02485) pilC 478337..479560 (-) 1224 WP_414931888.1 type II secretion system F family protein Machinery gene
  ACMVO4_RS02490 (ACMVO4_02490) pilB 479571..481262 (-) 1692 WP_414931889.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMVO4_RS02495 (ACMVO4_02495) pilA 481268..481702 (-) 435 WP_069667590.1 type IV pilin protein Machinery gene
  ACMVO4_RS02500 (ACMVO4_02500) nadC 481944..482831 (-) 888 WP_069667589.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMVO4_RS02505 (ACMVO4_02505) ampD 482963..483508 (+) 546 WP_272249761.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACMVO4_RS02510 (ACMVO4_02510) fldB 483650..484171 (-) 522 WP_004742606.1 flavodoxin FldB -
  ACMVO4_RS02515 (ACMVO4_02515) xerD 484335..485231 (+) 897 WP_414932277.1 site-specific tyrosine recombinase XerD -
  ACMVO4_RS02520 (ACMVO4_02520) - 485272..486024 (+) 753 WP_414931890.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62463.43 Da        Isoelectric Point: 5.4959

>NTDB_id=1094501 ACMVO4_RS02490 WP_414931889.1 479571..481262(-) (pilB) [Vibrio europaeus strain EX1]
MLTNLPSILRQAELLDPVQEAQLVKLVKDTDLTVAEVIIEQGWLTQAELASQLSRIFALPIIDFANYDYQTVCHNLGLRE
LITRSRAVPIRQSEHQLLIAVTDPTNIAIEEEFRFATGKQIELVLADIKQLNGAVRRLYGSAICGEEASHAKELSEQELV
SLVELSNDEINPVEDLSSDHSPVSRYINQILLDAVRKQASDIHFEPYEASYRVRFRCDGILIETQQPSHTLSRRLSARLK
IIAKLDIAERRLPQDGRIKLQLSASTAVDIRISTLPTLWGEKIVLRLLDSSTAELNIQSLGYNQHQQALYQKALSKPQGM
ILMTGPTGSGKTVSLYAGLNMLNTPQVNIATAEDPVEINLAGVNQVQINPKIGLDFAQALRAFLRQDPDIVMVGEIRDFE
TAEIAVKAAQTGHLVLSTLHTNSAAESVVRLQHMGISPFNVSSSLNLIIAQRLARKLCNHCKQQQTLPETHCQQLELANN
SVVFAANEQGCSECNQGYFGRTGIYEVMQINDVLVHVINQEADAKAIENVAIKQGMQTLKESGTEKLLAGVTSYQELQRV
LYL

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=1094501 ACMVO4_RS02490 WP_414931889.1 479571..481262(-) (pilB) [Vibrio europaeus strain EX1]
ATGCTAACCAACCTACCCTCTATTTTGCGCCAAGCTGAGCTTCTTGACCCTGTTCAAGAAGCTCAGTTAGTTAAGTTGGT
TAAAGATACTGACCTTACCGTAGCCGAAGTCATAATAGAGCAAGGTTGGCTCACCCAAGCGGAGCTAGCCTCACAACTCT
CGCGAATTTTTGCCTTACCCATCATCGACTTTGCAAATTACGATTATCAAACGGTATGCCACAACTTAGGCCTGCGTGAA
CTGATCACCCGCTCCCGAGCAGTACCTATTAGGCAGAGTGAACACCAACTGCTTATCGCCGTTACCGACCCAACCAATAT
CGCAATCGAAGAAGAGTTTCGCTTTGCAACCGGAAAGCAAATTGAGCTAGTTTTAGCTGACATAAAGCAGCTCAATGGCG
CGGTACGTCGCTTGTATGGCAGCGCTATCTGTGGTGAAGAAGCGAGTCATGCAAAAGAGCTGAGCGAACAAGAGCTCGTC
AGCCTAGTCGAGCTCTCTAATGATGAGATTAACCCAGTCGAAGACCTGAGTAGTGATCACTCACCAGTCAGCCGTTATAT
CAATCAAATCTTATTAGATGCGGTTAGGAAGCAAGCTTCTGATATCCACTTTGAACCCTATGAAGCCTCTTATCGTGTGC
GCTTTCGCTGTGATGGCATTTTGATTGAGACTCAGCAACCTAGCCATACATTAAGTCGCCGTTTATCCGCAAGACTAAAG
ATCATCGCCAAACTCGATATTGCCGAGCGCCGCTTACCTCAAGATGGGCGCATCAAGCTTCAACTAAGTGCAAGTACCGC
TGTCGATATTCGTATTTCAACCCTACCAACTCTATGGGGCGAGAAGATCGTGCTCCGTCTTCTTGATAGCAGCACTGCCG
AGTTAAACATTCAATCACTAGGGTACAATCAACACCAACAAGCTTTGTATCAAAAGGCTTTGAGCAAGCCCCAAGGCATG
ATTTTGATGACAGGACCAACAGGCAGCGGTAAGACAGTCTCACTTTATGCGGGGTTAAACATGCTCAATACCCCACAAGT
GAACATCGCCACCGCAGAAGACCCGGTGGAAATTAATTTAGCGGGCGTTAACCAAGTTCAAATCAACCCTAAAATTGGAT
TAGACTTCGCCCAAGCGTTACGCGCCTTCCTACGCCAAGACCCGGACATTGTCATGGTTGGTGAGATACGCGACTTCGAA
ACTGCTGAAATCGCAGTCAAAGCGGCACAAACAGGTCACCTAGTGCTATCAACATTGCATACGAACTCGGCTGCCGAAAG
CGTCGTGCGCTTGCAACATATGGGCATCTCCCCATTCAATGTCTCTTCATCATTGAACTTAATCATTGCCCAGCGTTTGG
CACGAAAGCTGTGTAACCATTGTAAGCAGCAGCAAACCTTGCCCGAGACACATTGCCAACAACTGGAGTTAGCAAACAAC
AGCGTGGTTTTTGCTGCTAATGAGCAAGGGTGCAGTGAATGCAATCAAGGCTACTTTGGGCGAACCGGAATCTATGAAGT
GATGCAGATAAACGATGTGTTAGTGCACGTCATCAATCAAGAGGCAGATGCTAAAGCCATCGAAAACGTCGCGATAAAGC
AAGGTATGCAGACGCTTAAAGAATCTGGAACGGAGAAACTGCTCGCAGGAGTGACCAGCTACCAAGAGTTGCAACGTGTT
CTTTACTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

63.943

100

0.639
  pilB Vibrio parahaemolyticus RIMD 2210633

61.837

100

0.622
  pilB Vibrio campbellii strain DS40M4

61.279

100

0.613
  pilB Acinetobacter baumannii D1279779

48.502

94.849

0.46
  pilB Legionella pneumophila strain ERS1305867

46.057

99.112

0.456
  pilB Acinetobacter baylyi ADP1

47.081

94.316

0.444
  pilF Neisseria gonorrhoeae MS11

44.173

94.494

0.417
  pilB Haemophilus influenzae 86-028NP

42.827

85.435

0.366
  pilB Haemophilus influenzae Rd KW20

42.268

86.146

0.364
  pilB/pilB1 Synechocystis sp. PCC 6803

38.889

92.718

0.361
  pilF Thermus thermophilus HB27

37.873

95.204

0.361

Multiple sequence alignment