Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACLUPT_RS03385 Genome accession   NZ_CP179718
Coordinates   719302..719748 (+) Length   148 a.a.
NCBI ID   WP_337250112.1    Uniprot ID   -
Organism   Variovorax sp. SCN45     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714302..724748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLUPT_RS03365 (ACLUPT_03365) - 714446..715948 (-) 1503 WP_237678309.1 DHA2 family efflux MFS transporter permease subunit -
  ACLUPT_RS03370 (ACLUPT_03370) - 716095..717405 (+) 1311 WP_237678310.1 putative zinc-binding metallopeptidase -
  ACLUPT_RS03375 (ACLUPT_03375) - 717396..717635 (-) 240 WP_237678311.1 cysteine-rich CWC family protein -
  ACLUPT_RS03380 (ACLUPT_03380) - 717635..718900 (-) 1266 WP_237678312.1 6-phosphofructokinase -
  ACLUPT_RS03385 (ACLUPT_03385) pilA/pilAI 719302..719748 (+) 447 WP_337250112.1 pilin Machinery gene
  ACLUPT_RS03390 (ACLUPT_03390) - 719870..720346 (+) 477 WP_237678313.1 tetratricopeptide repeat protein -
  ACLUPT_RS03395 (ACLUPT_03395) - 720551..721354 (-) 804 WP_237678314.1 enoyl-CoA hydratase-related protein -
  ACLUPT_RS03400 (ACLUPT_03400) - 721360..722538 (-) 1179 WP_237678315.1 acetyl-CoA C-acetyltransferase -
  ACLUPT_RS03405 (ACLUPT_03405) - 722674..723006 (-) 333 WP_237678316.1 DUF2917 domain-containing protein -
  ACLUPT_RS03410 (ACLUPT_03410) - 723089..724015 (+) 927 WP_237678317.1 LysR substrate-binding domain-containing protein -
  ACLUPT_RS03415 (ACLUPT_03415) - 724023..724601 (-) 579 WP_237678318.1 fasciclin domain-containing protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15374.75 Da        Isoelectric Point: 8.4989

>NTDB_id=1092806 ACLUPT_RS03385 WP_337250112.1 719302..719748(+) (pilA/pilAI) [Variovorax sp. SCN45]
MNRSTMRIAQNGFTLIELMIVVAIIGILAAVALPAYQDYTAKSQIAASLAEITPGKVMVETKITDGEAVSTATAIGLQTA
TKRCTAITPTFTVANKGAGTISCSMIGGTQVNGKTITWTRSADDATTNPGSWACTTTVDAKLKPKECS

Nucleotide


Download         Length: 447 bp        

>NTDB_id=1092806 ACLUPT_RS03385 WP_337250112.1 719302..719748(+) (pilA/pilAI) [Variovorax sp. SCN45]
ATGAACCGTTCAACGATGCGCATTGCGCAAAATGGCTTCACTTTGATCGAATTGATGATCGTCGTGGCAATCATTGGTAT
TCTTGCTGCTGTTGCGCTGCCTGCCTATCAAGACTACACAGCGAAGTCGCAAATCGCGGCTTCGCTGGCAGAAATCACGC
CTGGCAAGGTGATGGTCGAAACAAAAATTACTGATGGCGAAGCCGTAAGCACTGCCACCGCGATTGGCCTCCAAACCGCT
ACAAAACGTTGTACTGCAATTACCCCCACGTTTACTGTTGCGAATAAGGGTGCTGGCACTATTTCGTGTTCAATGATTGG
AGGCACACAAGTGAATGGTAAAACAATCACTTGGACGCGATCGGCGGATGACGCTACCACCAATCCAGGCTCGTGGGCGT
GCACGACTACCGTCGATGCGAAGCTTAAGCCAAAGGAATGCTCTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.345

97.973

0.493
  pilA Pseudomonas aeruginosa PAK

43.59

100

0.459
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.455

96.622

0.439
  pilA2 Legionella pneumophila strain ERS1305867

42

100

0.426
  pilA2 Legionella pneumophila str. Paris

42

100

0.426
  comP Acinetobacter baylyi ADP1

41.892

100

0.419
  pilA Vibrio cholerae C6706

39.355

100

0.412
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.355

100

0.412
  pilA Vibrio cholerae strain A1552

39.355

100

0.412
  pilA Acinetobacter baumannii strain A118

40.426

95.27

0.385
  pilA Ralstonia pseudosolanacearum GMI1000

31.609

100

0.372

Multiple sequence alignment