Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLF6L_RS03200 Genome accession   NZ_CP178333
Coordinates   717982..719190 (-) Length   402 a.a.
NCBI ID   WP_238617412.1    Uniprot ID   -
Organism   Ectothiorhodospira shaposhnikovii strain PHS-Q     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712982..724190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6L_RS03175 (ACLF6L_03175) - 714104..715069 (+) 966 WP_238617408.1 Nudix family hydrolase -
  ACLF6L_RS03180 (ACLF6L_03180) - 715126..715590 (+) 465 WP_412852354.1 hypothetical protein -
  ACLF6L_RS03185 (ACLF6L_03185) zapD 715616..716458 (-) 843 WP_412852355.1 cell division protein ZapD -
  ACLF6L_RS03190 (ACLF6L_03190) coaE 716504..717103 (-) 600 WP_412852356.1 dephospho-CoA kinase -
  ACLF6L_RS03195 (ACLF6L_03195) pilD 717107..717922 (-) 816 WP_238617487.1 prepilin peptidase Machinery gene
  ACLF6L_RS03200 (ACLF6L_03200) pilC 717982..719190 (-) 1209 WP_238617412.1 type II secretion system F family protein Machinery gene
  ACLF6L_RS03205 (ACLF6L_03205) pilB 719194..720906 (-) 1713 WP_238617413.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLF6L_RS03210 (ACLF6L_03210) pilR 721056..722420 (-) 1365 WP_238617414.1 sigma-54-dependent transcriptional regulator Regulator
  ACLF6L_RS03215 (ACLF6L_03215) - 722417..724087 (-) 1671 WP_412852357.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43766.31 Da        Isoelectric Point: 8.3083

>NTDB_id=1088884 ACLF6L_RS03200 WP_238617412.1 717982..719190(-) (pilC) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
MAETMTVFVWEGVDKKGVRIKGETTAANENLAKAELRRQGINPLKVRKKPKPLFGGKKKITPKDIAIFLRQMSTMMSAGV
PLVQSFEIVGRGHENPSMQELILRIKADVEGGATFASALARHPAYFDDLVINLVGAGEQSGALETLLDRIATYKEKTESL
KAKIKKAMFYPAAVIVVAFIVTAILLIFVVPQFQDLFQGFGADLPVFTQFVIGISEFFQSYWWAIFGGIGAAVFGFTQAK
QRSAAFRRQLDLLSLRIPVVGELLRKAAIARFARTLSTMFSAGVPLVEAMESVAGATGNALFSEATLRMRDETAAGTQLQ
QAMRNSAVFPNMVVQMVAIGEESGSLDEMLGKVADFYEEEVDNMVDGLSSLMEPMIMAFLGIVVGGLVIAMYLPIFQLGA
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1088884 ACLF6L_RS03200 WP_238617412.1 717982..719190(-) (pilC) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
ATGGCCGAGACAATGACGGTGTTCGTCTGGGAAGGGGTGGACAAGAAAGGCGTCCGGATCAAGGGCGAGACCACCGCCGC
CAACGAAAATCTGGCCAAGGCGGAACTGCGGCGCCAGGGTATCAACCCGCTCAAGGTACGCAAGAAACCCAAGCCGCTGT
TCGGCGGCAAGAAGAAGATCACCCCCAAGGACATCGCCATCTTCCTGCGCCAGATGTCCACCATGATGAGCGCCGGCGTG
CCCCTGGTGCAGTCCTTCGAGATCGTCGGCCGGGGCCACGAAAATCCCTCCATGCAGGAACTGATCCTACGAATCAAGGC
GGACGTGGAAGGGGGTGCCACCTTTGCCAGCGCCCTGGCCAGGCACCCGGCCTACTTCGACGACCTGGTCATCAACCTGG
TGGGGGCCGGTGAACAGTCCGGCGCCCTGGAAACCCTGCTGGATCGCATTGCCACCTACAAGGAAAAGACCGAATCCCTC
AAGGCCAAGATCAAGAAGGCCATGTTCTATCCCGCCGCCGTGATCGTGGTGGCCTTCATCGTCACCGCCATCCTGCTCAT
CTTCGTGGTTCCCCAGTTTCAGGACCTGTTCCAGGGCTTTGGCGCAGACCTGCCCGTCTTCACCCAGTTCGTCATCGGTA
TCTCCGAGTTCTTCCAGAGCTACTGGTGGGCCATCTTCGGCGGTATCGGCGCCGCGGTGTTCGGGTTCACCCAGGCCAAG
CAGCGTTCCGCCGCCTTCCGCCGTCAGCTCGACCTGCTCTCCCTGCGCATCCCGGTGGTGGGCGAACTGCTGCGCAAGGC
CGCCATTGCCCGCTTTGCCCGGACCCTGTCCACCATGTTCTCCGCCGGTGTCCCCCTGGTGGAAGCGATGGAGTCGGTGG
CCGGCGCGACGGGCAACGCCCTCTTTTCCGAAGCGACCCTGCGCATGCGCGACGAAACCGCGGCCGGCACCCAGCTCCAG
CAGGCCATGCGGAATTCCGCGGTATTTCCCAACATGGTGGTACAGATGGTGGCCATCGGGGAAGAATCCGGTTCCCTGGA
CGAAATGCTGGGCAAGGTCGCCGATTTCTATGAAGAGGAAGTGGACAACATGGTCGACGGCCTCTCCAGCCTCATGGAAC
CCATGATCATGGCCTTCCTGGGCATTGTCGTCGGCGGTCTGGTCATTGCCATGTATCTTCCCATCTTCCAGCTTGGAGCC
GTGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.469

98.756

0.617
  pilC Acinetobacter baumannii D1279779

60.298

100

0.604
  pilC Acinetobacter baylyi ADP1

59.95

100

0.6
  pilC Legionella pneumophila strain ERS1305867

57.644

99.254

0.572
  pilG Neisseria gonorrhoeae MS11

46.97

98.507

0.463
  pilG Neisseria meningitidis 44/76-A

46.717

98.507

0.46
  pilC Vibrio cholerae strain A1552

42.929

98.507

0.423
  pilC Vibrio campbellii strain DS40M4

42.462

99.005

0.42
  pilC Thermus thermophilus HB27

37.56

100

0.391

Multiple sequence alignment