Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLF6L_RS03205 Genome accession   NZ_CP178333
Coordinates   719194..720906 (-) Length   570 a.a.
NCBI ID   WP_238617413.1    Uniprot ID   -
Organism   Ectothiorhodospira shaposhnikovii strain PHS-Q     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 714194..725906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6L_RS03180 (ACLF6L_03180) - 715126..715590 (+) 465 WP_412852354.1 hypothetical protein -
  ACLF6L_RS03185 (ACLF6L_03185) zapD 715616..716458 (-) 843 WP_412852355.1 cell division protein ZapD -
  ACLF6L_RS03190 (ACLF6L_03190) coaE 716504..717103 (-) 600 WP_412852356.1 dephospho-CoA kinase -
  ACLF6L_RS03195 (ACLF6L_03195) pilD 717107..717922 (-) 816 WP_238617487.1 prepilin peptidase Machinery gene
  ACLF6L_RS03200 (ACLF6L_03200) pilC 717982..719190 (-) 1209 WP_238617412.1 type II secretion system F family protein Machinery gene
  ACLF6L_RS03205 (ACLF6L_03205) pilB 719194..720906 (-) 1713 WP_238617413.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLF6L_RS03210 (ACLF6L_03210) pilR 721056..722420 (-) 1365 WP_238617414.1 sigma-54-dependent transcriptional regulator Regulator
  ACLF6L_RS03215 (ACLF6L_03215) - 722417..724087 (-) 1671 WP_412852357.1 sensor histidine kinase -
  ACLF6L_RS03220 (ACLF6L_03220) - 724135..724386 (-) 252 WP_238617416.1 PP0621 family protein -
  ACLF6L_RS03225 (ACLF6L_03225) sucC 724524..725693 (+) 1170 WP_238617417.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62954.24 Da        Isoelectric Point: 5.0890

>NTDB_id=1088885 ACLF6L_RS03205 WP_238617413.1 719194..720906(-) (pilB) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
MSTPAASIPLSGLARRLVQDGLLSEADANKALDQSRKKRVPLVAYLVTEKIVEARGLAQAAAEEFGTPLFDLDALDKEYL
PTKLVDEKLIRQHQALPIFRRGNRLFVGMSDPMNISALDEIKFNTGIPTEPVLVEHDKLSKLIEDSLNDASSMMEGLMDE
DLDNLEMTDTEDRGPEVSESEVDDTPVVRFVNKVLLDAINRKASDIHFEPYEKDFRVRFRLDGVLDEVAHPPVNLAPRII
ARIKVMSRMDISERRVPQDGRIKMQLSKNRAIDFRVNTCPTLYGEKVVLRILDPTSAFLGIDALGYEEHQKALYLNALSK
PYGMILVTGPTGSGKTVSLYTGLGILNTPDRNISTAEDPAEINMPGINQVNVNPKVGLTFAAALRAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPSYNIASSILLIIAQRLARRLCNHCKAPEDVPKEVLLQEG
FTEDEVNQGFKIFRPVGCDQCTNGYKGRVGIYQVLPISEAMQRIILDGGNSMQLAEQARAEGVDDLRRAGLLKVKAGVTS
LEEVNRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1088885 ACLF6L_RS03205 WP_238617413.1 719194..720906(-) (pilB) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
ATGTCCACACCTGCCGCCTCCATTCCATTGAGCGGGCTTGCCCGGCGCCTGGTTCAAGACGGATTGCTCAGTGAAGCCGA
CGCCAACAAGGCACTGGATCAGTCCCGCAAGAAACGGGTACCCCTGGTGGCCTACCTGGTGACGGAAAAGATCGTGGAGG
CCCGCGGCTTGGCCCAGGCGGCGGCCGAAGAGTTCGGTACCCCCCTGTTCGATCTGGACGCCCTCGACAAGGAATACCTG
CCCACCAAACTGGTGGACGAGAAGCTGATCCGCCAGCATCAGGCCCTGCCCATCTTCCGGCGGGGTAATCGGCTGTTCGT
GGGCATGTCCGATCCCATGAACATCAGCGCCCTGGATGAAATCAAGTTCAACACCGGCATCCCCACCGAACCGGTGCTGG
TGGAACACGACAAGTTGTCCAAGCTGATTGAGGACAGCCTCAACGACGCCTCCTCGATGATGGAAGGCCTCATGGACGAG
GACCTGGACAACCTGGAGATGACGGACACCGAAGACCGCGGCCCGGAGGTTTCCGAAAGCGAAGTGGACGACACGCCGGT
GGTCCGCTTCGTCAACAAGGTGCTGCTGGATGCCATCAACCGCAAGGCCTCGGACATCCACTTCGAACCCTATGAAAAGG
ACTTTCGGGTGCGGTTCCGCCTGGACGGGGTACTCGACGAGGTGGCCCACCCACCGGTGAACCTGGCCCCCCGGATCATC
GCCCGCATCAAGGTGATGTCCCGCATGGACATCTCCGAACGACGCGTCCCTCAGGACGGGCGCATCAAGATGCAGCTCTC
CAAGAACCGGGCCATCGACTTTCGTGTCAACACCTGCCCCACCCTTTACGGAGAAAAGGTGGTGCTGCGAATACTGGACC
CCACCAGCGCCTTCCTGGGTATCGACGCCCTGGGCTACGAAGAGCACCAGAAAGCACTCTATCTCAACGCCCTGAGCAAG
CCCTACGGCATGATCCTGGTCACCGGCCCTACCGGCTCGGGCAAGACCGTCTCGCTCTATACCGGCCTGGGCATCCTCAA
CACCCCGGACCGGAACATCTCCACGGCCGAGGATCCCGCCGAAATCAACATGCCGGGCATCAATCAGGTCAACGTGAATC
CCAAGGTGGGGCTGACCTTTGCCGCCGCCCTGAGGGCCTTTCTGCGGCAGGACCCCGACATCATCATGGTCGGCGAGATC
CGCGACCTGGAAACCGCCGAAATCGCCATCAAGGCGGCTCAGACCGGTCACCTGGTGCTATCCACCCTGCACACCAACGA
CGCACCCCAAACCCTCACCCGCCTGGCCAACATGGGGGTCCCCTCTTACAACATCGCCTCATCCATCCTGCTCATCATCG
CCCAGCGTCTGGCCCGGCGGCTTTGCAACCATTGCAAGGCGCCCGAAGACGTCCCCAAGGAGGTGCTGTTGCAGGAGGGT
TTCACCGAAGACGAGGTGAACCAGGGCTTCAAGATCTTCAGGCCGGTGGGATGTGACCAGTGCACCAACGGCTACAAGGG
ACGGGTGGGCATCTACCAGGTATTGCCCATTTCCGAAGCCATGCAGCGGATCATTCTCGATGGCGGCAACTCGATGCAAC
TGGCGGAACAGGCCAGGGCGGAAGGCGTCGACGACCTCCGGCGGGCCGGTCTGCTCAAGGTCAAGGCCGGTGTCACCAGC
CTTGAAGAAGTCAACCGTGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-145)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.681

100

0.593
  pilB Acinetobacter baylyi ADP1

56.584

98.596

0.558
  pilB Legionella pneumophila strain ERS1305867

55.556

99.474

0.553
  pilF Neisseria gonorrhoeae MS11

50.177

98.947

0.496
  pilB Vibrio cholerae strain A1552

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.033

99.825

0.489
  pilB Vibrio campbellii strain DS40M4

47.872

98.947

0.474
  pilF Thermus thermophilus HB27

40.433

97.193

0.393
  pilB/pilB1 Synechocystis sp. PCC 6803

39.531

97.193

0.384

Multiple sequence alignment