Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLF6L_RS03195 Genome accession   NZ_CP178333
Coordinates   717107..717922 (-) Length   271 a.a.
NCBI ID   WP_238617487.1    Uniprot ID   -
Organism   Ectothiorhodospira shaposhnikovii strain PHS-Q     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 712107..722922
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLF6L_RS03170 (ACLF6L_03170) argJ 712884..714098 (+) 1215 WP_238617407.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACLF6L_RS03175 (ACLF6L_03175) - 714104..715069 (+) 966 WP_238617408.1 Nudix family hydrolase -
  ACLF6L_RS03180 (ACLF6L_03180) - 715126..715590 (+) 465 WP_412852354.1 hypothetical protein -
  ACLF6L_RS03185 (ACLF6L_03185) zapD 715616..716458 (-) 843 WP_412852355.1 cell division protein ZapD -
  ACLF6L_RS03190 (ACLF6L_03190) coaE 716504..717103 (-) 600 WP_412852356.1 dephospho-CoA kinase -
  ACLF6L_RS03195 (ACLF6L_03195) pilD 717107..717922 (-) 816 WP_238617487.1 prepilin peptidase Machinery gene
  ACLF6L_RS03200 (ACLF6L_03200) pilC 717982..719190 (-) 1209 WP_238617412.1 type II secretion system F family protein Machinery gene
  ACLF6L_RS03205 (ACLF6L_03205) pilB 719194..720906 (-) 1713 WP_238617413.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLF6L_RS03210 (ACLF6L_03210) pilR 721056..722420 (-) 1365 WP_238617414.1 sigma-54-dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 271 a.a.        Molecular weight: 29499.14 Da        Isoelectric Point: 7.7443

>NTDB_id=1088883 ACLF6L_RS03195 WP_238617487.1 717107..717922(-) (pilD) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
MVGSFLNVVIHRLPMMMERQWQSEARAWLQSRGGNLDHAEEVQGPYNLVIPRSACPACGHRITALENIPLISYLFLRGKC
ASCGARISPQYPLVEMATALLSMAVVWHFGVTWVAAAALLLTWALIALTVIDLRTTLLPDVITLPLLWLGLVLNLGGLFT
DATSSLLGAVFGYLSLWLVYHAFRLLTGKEGMGYGDFKLFAALGAWLGWQMLPLIILLSSLVGAVVGLVLIAVLGRDRNL
PIPFGPYLAAAGWIALLWGDTIVQTYLGMLG

Nucleotide


Download         Length: 816 bp        

>NTDB_id=1088883 ACLF6L_RS03195 WP_238617487.1 717107..717922(-) (pilD) [Ectothiorhodospira shaposhnikovii strain PHS-Q]
ATGGTGGGCAGCTTTCTCAATGTGGTCATCCACCGCCTGCCGATGATGATGGAACGCCAGTGGCAAAGCGAGGCAAGGGC
ATGGCTGCAGTCACGGGGAGGCAACCTGGATCATGCCGAAGAGGTCCAGGGACCCTATAACCTGGTCATCCCCCGGTCCG
CCTGTCCTGCCTGCGGTCACCGCATCACGGCCCTGGAGAACATCCCGCTCATCAGTTACCTGTTCCTCAGAGGCAAGTGC
GCCTCGTGCGGCGCCAGAATCTCTCCCCAGTACCCACTGGTGGAAATGGCCACGGCCCTGCTATCCATGGCCGTGGTCTG
GCATTTCGGTGTCACCTGGGTGGCCGCGGCGGCCCTGCTGCTGACCTGGGCCCTGATTGCCCTGACCGTGATCGACCTGC
GCACCACCCTGTTGCCGGATGTCATCACCCTGCCCCTGCTGTGGCTGGGACTGGTACTGAATCTGGGAGGGCTGTTCACC
GATGCCACCAGCAGCCTGCTGGGGGCCGTCTTCGGTTACCTGAGCCTGTGGCTCGTCTACCATGCCTTCCGATTGCTCAC
CGGCAAGGAAGGCATGGGCTACGGTGACTTCAAGCTCTTTGCCGCCCTGGGCGCCTGGCTGGGCTGGCAGATGCTCCCCC
TGATCATCCTGCTGTCCTCCCTGGTGGGTGCCGTGGTGGGTCTGGTGCTCATCGCCGTGCTGGGACGTGACCGCAATCTG
CCCATTCCCTTCGGTCCCTATCTGGCTGCCGCGGGCTGGATTGCCCTGCTCTGGGGCGACACCATCGTACAGACCTACCT
CGGCATGCTCGGCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(1-109)

Type IV leader peptidase family (Peptidase_A24)

(120-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

52.809

98.524

0.52
  pilD Acinetobacter baumannii D1279779

52.434

98.524

0.517
  pilD Vibrio cholerae strain A1552

51.866

98.893

0.513
  pilD Vibrio campbellii strain DS40M4

50.558

99.262

0.502
  pilD Neisseria gonorrhoeae MS11

50

96.679

0.483

Multiple sequence alignment