Detailed information NTG1082833

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACLH0I_RS18240
• Genome accession: NZ_CP176770
• Coordinates: 4276563..4277681 (-)
• Length: 372 a.a.
• NCBI ID: WP_411385012.1
• Uniprot ID: -
• Organism: Pseudomonas sp. MPB03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4271563..4282681

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLH0I_RS18225 (ACLH0I_18225)	-	4272068..4273459 (+)	1392	WP_186706698.1	GntP family permease	-
ACLH0I_RS18230 (ACLH0I_18230)	-	4273468..4274244 (+)	777	WP_250965432.1	3-hydroxybutyrate dehydrogenase	-
ACLH0I_RS18235 (ACLH0I_18235)	-	4274472..4276427 (+)	1956	WP_411385011.1	acetoacetate--CoA ligase	-
ACLH0I_RS18240 (ACLH0I_18240)	pilU	4276563..4277681 (-)	1119	WP_411385012.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACLH0I_RS18245 (ACLH0I_18245)	-	4277814..4280351 (+)	2538	WP_411385013.1	PAS domain-containing protein	-
ACLH0I_RS18250 (ACLH0I_18250)	-	4280400..4280675 (+)	276	WP_003182808.1	peptidylprolyl isomerase	-
ACLH0I_RS18255 (ACLH0I_18255)	-	4280823..4281740 (-)	918	WP_411385014.1	sugar kinase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41418.41 Da
• Isoelectric Point: 6.2516

>NTDB_id=1082833 ACLH0I_RS18240 WP_411385012.1 4276563..4277681(-) (pilU) [Pseudomonas sp. MPB03]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVIKPLANEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKSIMEKSEELGMQTFDQAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHTESGAAAPPADPAAGEWGLMD

Nucleotide

• Length: 1119 bp

>NTDB_id=1082833 ACLH0I_RS18240 WP_411385012.1 4276563..4277681(-) (pilU) [Pseudomonas sp. MPB03]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGCGCGCCGCCATG
CGCGCGTTTCGAAGGGGTGATCAAGCCCTTGGCTAACGAGGCCTTCAAGGTCGGCGACATTGCCCGTCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAATTGGAAATGAACCTGGCGATTTCCCTGGCGGGCGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAACGACGTGTCCATGGTGATTCGCAACGTCAAGCTGGATATCCCGCGCTTCGA
AGACCTGAAACTGCCCCGGGTGTTGTTGGACACCATCATGCAGAAACAGGGCTTGATGCTGTTCGTCGGTGCGACAGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGTCACATCATCACCATCGAGGAT
CCGGTGGAGTACATCCATCGGCATAAGAAATCCATCATCAACCAACGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
CGCCCTGAAGAACACGTTGCGCCAGGCACCGGATGTGGTGCTGATCGGCGAGATCCGCGATCGGGAAACCATGGAGCATG
CCCTGGCTTTTGCCGACACCGGGCACCTGGTGATTTCCACCTTGCATGCCCATAACGCCAACCAGGCCTTGGACCGGGTG
ATCAATTTCTTCCCGGAGGAACGCCGGCCGCAGTTATTGAACGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
TCTGGTACGCACCCGCACTGGCCAGCGGCGGGCTGCGGTGGAAGTGATGCTGGGTTCACCGACGGTGGCCGACCTGATCC
GGCGCAACGAACTTGGCGAGCTCAAGTCCATCATGGAAAAGTCGGAGGAACTGGGCATGCAGACTTTTGACCAGGCGCTG
TTCAACCTGGTAGTCGAAGGGGCCATCGACGAGGAAGAAGCGCTGAAAAATGCCGACTCGGCGAACAACCTGCGGTTGCG
TTTGAAGCTGCATACCGAGTCGGGCGCGGCGGCGCCACCGGCTGATCCAGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	95.43	0.54
pilU	Acinetobacter baylyi ADP1	52.247	95.699	0.5
pilU	Vibrio cholerae strain A1552	52.571	94.086	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.525	98.387	0.379
pilT	Vibrio cholerae strain A1552	41.141	89.516	0.368
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.516	0.368
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.473	0.366
pilT	Acinetobacter nosocomialis M2	40.419	89.785	0.363
pilT	Acinetobacter baumannii D1279779	40.419	89.785	0.363
pilT	Acinetobacter baumannii strain A118	40.419	89.785	0.363
pilT	Pseudomonas aeruginosa PAK	39.244	92.473	0.363