Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   ACK2JZ_RS13095 Genome accession   NZ_CP176623
Coordinates   2723077..2723352 (-) Length   91 a.a.
NCBI ID   WP_002380445.1    Uniprot ID   -
Organism   Enterococcus faecalis strain SFAD86     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2677230..2741458 2723077..2723352 within 0


Gene organization within MGE regions


Location: 2677230..2741458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2JZ_RS12790 - 2677230..2678237 (-) 1008 WP_002380426.1 class I SAM-dependent methyltransferase -
  ACK2JZ_RS12795 comGG 2678366..2678719 (-) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  ACK2JZ_RS12800 comGF 2678719..2679153 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  ACK2JZ_RS12805 - 2679143..2679514 (-) 372 WP_171804888.1 type II secretion system protein -
  ACK2JZ_RS12810 - 2679848..2679973 (+) 126 WP_002364184.1 hypothetical protein -
  ACK2JZ_RS12815 hemH 2680270..2681211 (-) 942 WP_002364185.1 ferrochelatase -
  ACK2JZ_RS12825 - 2681955..2682257 (-) 303 WP_257409305.1 hypothetical protein -
  ACK2JZ_RS12830 - 2682329..2682529 (-) 201 WP_002357053.1 cold-shock protein -
  ACK2JZ_RS12835 - 2683366..2684607 (-) 1242 WP_002394567.1 LysM peptidoglycan-binding domain-containing protein -
  ACK2JZ_RS12840 - 2684608..2684841 (-) 234 WP_002384371.1 phage holin -
  ACK2JZ_RS12845 - 2684834..2685055 (-) 222 WP_002364191.1 hypothetical protein -
  ACK2JZ_RS12850 - 2685090..2685245 (-) 156 WP_002364192.1 XkdX family protein -
  ACK2JZ_RS12855 - 2685247..2685567 (-) 321 WP_002389262.1 hypothetical protein -
  ACK2JZ_RS12860 - 2685581..2686072 (-) 492 WP_002364194.1 hypothetical protein -
  ACK2JZ_RS12865 - 2686072..2686359 (-) 288 WP_002357046.1 collagen-like protein -
  ACK2JZ_RS12870 - 2686356..2686952 (-) 597 WP_002357045.1 hypothetical protein -
  ACK2JZ_RS12875 - 2686945..2687826 (-) 882 WP_002357044.1 phage baseplate upper protein -
  ACK2JZ_RS12880 ltrA 2688104..2689915 (-) 1812 WP_065535846.1 group II intron reverse transcriptase/maturase -
  ACK2JZ_RS12885 - 2690597..2693299 (-) 2703 WP_236645956.1 phage tail spike protein -
  ACK2JZ_RS12890 - 2693281..2694015 (-) 735 WP_002380434.1 hypothetical protein -
  ACK2JZ_RS12895 - 2694005..2696902 (-) 2898 WP_201751548.1 tape measure protein -
  ACK2JZ_RS12900 gpG 2697150..2697500 (-) 351 WP_002357039.1 phage tail assembly chaperone G -
  ACK2JZ_RS12905 - 2697553..2698401 (-) 849 WP_002384363.1 major tail protein -
  ACK2JZ_RS12910 - 2698402..2698776 (-) 375 WP_002357037.1 DUF6838 family protein -
  ACK2JZ_RS12915 - 2698779..2699177 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  ACK2JZ_RS12920 - 2699170..2699538 (-) 369 WP_002357034.1 hypothetical protein -
  ACK2JZ_RS12925 - 2699535..2699879 (-) 345 WP_002357033.1 hypothetical protein -
  ACK2JZ_RS12930 - 2699893..2700075 (-) 183 WP_002357032.1 hypothetical protein -
  ACK2JZ_RS12935 - 2700104..2700991 (-) 888 WP_002357030.1 DUF5309 domain-containing protein -
  ACK2JZ_RS12940 - 2701005..2701628 (-) 624 WP_002393977.1 DUF4355 domain-containing protein -
  ACK2JZ_RS12945 - 2701847..2702167 (-) 321 WP_002380436.1 hypothetical protein -
  ACK2JZ_RS12950 - 2702224..2702454 (-) 231 WP_002380437.1 hypothetical protein -
  ACK2JZ_RS12955 - 2702455..2704359 (-) 1905 WP_002393613.1 hypothetical protein -
  ACK2JZ_RS12960 - 2704334..2705821 (-) 1488 WP_002393614.1 phage portal protein -
  ACK2JZ_RS12965 - 2705833..2707122 (-) 1290 WP_002393615.1 PBSX family phage terminase large subunit -
  ACK2JZ_RS12970 terS 2707094..2707898 (-) 805 Protein_2539 phage terminase small subunit -
  ACK2JZ_RS12975 - 2707945..2708661 (-) 717 WP_002372040.1 hypothetical protein -
  ACK2JZ_RS12980 - 2708754..2708981 (-) 228 WP_002372042.1 hypothetical protein -
  ACK2JZ_RS12990 - 2711079..2711495 (-) 417 WP_002372045.1 ArpU family phage packaging/lysis transcriptional regulator -
  ACK2JZ_RS12995 - 2712402..2712836 (-) 435 WP_002372046.1 RusA family crossover junction endodeoxyribonuclease -
  ACK2JZ_RS13000 - 2712845..2713144 (-) 300 WP_002372047.1 MazG-like family protein -
  ACK2JZ_RS13005 - 2713145..2713447 (-) 303 WP_002368214.1 hypothetical protein -
  ACK2JZ_RS13010 - 2713451..2714413 (-) 963 Protein_2546 Lin1244/Lin1753 domain-containing protein -
  ACK2JZ_RS13015 - 2714427..2715317 (-) 891 WP_002364218.1 recombinase RecT -
  ACK2JZ_RS13020 - 2715320..2716261 (-) 942 WP_002364219.1 YqaJ viral recombinase family protein -
  ACK2JZ_RS13025 - 2716359..2716586 (-) 228 WP_002364220.1 hypothetical protein -
  ACK2JZ_RS13030 - 2716586..2716909 (-) 324 WP_002380444.1 hypothetical protein -
  ACK2JZ_RS13035 - 2716953..2717138 (-) 186 WP_002364222.1 hypothetical protein -
  ACK2JZ_RS13040 - 2717129..2717323 (-) 195 WP_002364223.1 hypothetical protein -
  ACK2JZ_RS13045 - 2717376..2717558 (+) 183 WP_002364224.1 YegP family protein -
  ACK2JZ_RS13050 - 2717598..2718320 (-) 723 WP_002364225.1 Rha family transcriptional regulator -
  ACK2JZ_RS13055 - 2718359..2718676 (-) 318 WP_002364228.1 hypothetical protein -
  ACK2JZ_RS13060 - 2718682..2718873 (-) 192 WP_002356998.1 hypothetical protein -
  ACK2JZ_RS13065 - 2719164..2719508 (+) 345 WP_002385819.1 helix-turn-helix domain-containing protein -
  ACK2JZ_RS13070 - 2719513..2720160 (+) 648 WP_002372050.1 ImmA/IrrE family metallo-endopeptidase -
  ACK2JZ_RS13075 - 2720278..2721042 (+) 765 WP_002389030.1 LysM peptidoglycan-binding domain-containing protein -
  ACK2JZ_RS13080 - 2721057..2721386 (+) 330 WP_002369911.1 hypothetical protein -
  ACK2JZ_RS13085 - 2721461..2722609 (+) 1149 WP_002389015.1 tyrosine-type recombinase/integrase -
  ACK2JZ_RS13090 comGD 2722637..2723080 (-) 444 WP_002356992.1 competence type IV pilus minor pilin ComGD -
  ACK2JZ_RS13095 comGC/cglC 2723077..2723352 (-) 276 WP_002380445.1 competence type IV pilus major pilin ComGC Machinery gene
  ACK2JZ_RS13100 comGB 2723352..2724398 (-) 1047 WP_002369171.1 competence type IV pilus assembly protein ComGB -
  ACK2JZ_RS13105 comGA 2724355..2725323 (-) 969 WP_002364362.1 competence type IV pilus ATPase ComGA -
  ACK2JZ_RS13110 - 2725564..2726892 (-) 1329 WP_002362058.1 APC family permease -
  ACK2JZ_RS13115 rlmN 2727182..2728255 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ACK2JZ_RS13120 - 2728380..2730209 (-) 1830 WP_002393620.1 ABC transporter permease -
  ACK2JZ_RS13125 - 2730199..2730948 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  ACK2JZ_RS13130 - 2731066..2731767 (-) 702 WP_002364244.1 GntR family transcriptional regulator -
  ACK2JZ_RS13135 - 2731898..2734321 (-) 2424 WP_002380448.1 DNA translocase FtsK -
  ACK2JZ_RS13140 - 2734635..2735846 (-) 1212 WP_002380449.1 NAD(P)/FAD-dependent oxidoreductase -
  ACK2JZ_RS13145 - 2735875..2736780 (-) 906 WP_002360016.1 prenyltransferase -
  ACK2JZ_RS13150 - 2736902..2737882 (+) 981 WP_002360015.1 polyprenyl synthetase family protein -
  ACK2JZ_RS13155 cydC 2737962..2739728 (-) 1767 WP_002381710.1 thiol reductant ABC exporter subunit CydC -
  ACK2JZ_RS13160 cydD 2739725..2741458 (-) 1734 WP_065536070.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10465.36 Da        Isoelectric Point: 7.0118

>NTDB_id=1081588 ACK2JZ_RS13095 WP_002380445.1 2723077..2723352(-) (comGC/cglC) [Enterococcus faecalis strain SFAD86]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDEKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=1081588 ACK2JZ_RS13095 WP_002380445.1 2723077..2723352(-) (comGC/cglC) [Enterococcus faecalis strain SFAD86]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATGAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

56.977

94.505

0.538
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus suis isolate S10

51.163

94.505

0.484
  comGC Latilactobacillus sakei subsp. sakei 23K

45.055

100

0.451
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

47.297

81.319

0.385

Multiple sequence alignment