Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   ACK2T8_RS12525 Genome accession   NZ_CP176513
Coordinates   2439282..2439686 (-) Length   134 a.a.
NCBI ID   WP_036658655.1    Uniprot ID   -
Organism   Paenibacillus larvae subsp. larvae strain B-3650     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2405319..2447784 2439282..2439686 within 0


Gene organization within MGE regions


Location: 2405319..2447784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2T8_RS12270 - 2405319..2405870 (+) 552 WP_036653975.1 DUF4352 domain-containing protein -
  ACK2T8_RS12275 - 2406081..2406239 (-) 159 WP_077584891.1 putative holin-like toxin -
  ACK2T8_RS12280 - 2406340..2406798 (-) 459 WP_040931299.1 helix-turn-helix domain-containing protein -
  ACK2T8_RS12285 - 2407036..2407221 (+) 186 WP_036653970.1 hypothetical protein -
  ACK2T8_RS12290 - 2407307..2407570 (+) 264 WP_036653966.1 hypothetical protein -
  ACK2T8_RS12295 - 2407882..2410800 (-) 2919 WP_357683092.1 RICIN domain-containing protein -
  ACK2T8_RS12305 - 2412828..2413208 (-) 381 WP_309556067.1 hypothetical protein -
  ACK2T8_RS12310 - 2413223..2413609 (-) 387 WP_309556066.1 hypothetical protein -
  ACK2T8_RS12315 - 2413629..2414042 (-) 414 WP_309556065.1 hypothetical protein -
  ACK2T8_RS12320 - 2414091..2414597 (-) 507 WP_309556064.1 hypothetical protein -
  ACK2T8_RS12325 - 2414784..2415038 (-) 255 WP_051428122.1 hypothetical protein -
  ACK2T8_RS12330 - 2415500..2415997 (-) 498 Protein_2400 N-acetylmuramoyl-L-alanine amidase family protein -
  ACK2T8_RS12335 - 2415997..2416236 (-) 240 WP_036656495.1 BhlA/UviB family holin-like peptide -
  ACK2T8_RS12340 - 2416217..2416378 (-) 162 WP_268568332.1 hypothetical protein -
  ACK2T8_RS12345 - 2416375..2416713 (-) 339 WP_268568333.1 hypothetical protein -
  ACK2T8_RS12350 - 2416723..2417796 (-) 1074 WP_275038512.1 hypothetical protein -
  ACK2T8_RS12355 - 2417802..2418920 (-) 1119 WP_309556063.1 siphovirus ReqiPepy6 Gp37-like family protein -
  ACK2T8_RS12360 - 2418923..2419774 (-) 852 WP_268568335.1 phage tail family protein -
  ACK2T8_RS12365 - 2419771..2422407 (-) 2637 WP_268568336.1 phage tail tape measure protein -
  ACK2T8_RS12370 - 2422464..2422718 (-) 255 WP_268568337.1 hypothetical protein -
  ACK2T8_RS12375 - 2422745..2423083 (-) 339 WP_268568338.1 hypothetical protein -
  ACK2T8_RS12380 - 2423083..2423514 (-) 432 WP_313781392.1 phage major tail protein, TP901-1 family -
  ACK2T8_RS12385 - 2423517..2423912 (-) 396 WP_268568340.1 DUF3168 domain-containing protein -
  ACK2T8_RS12390 - 2423896..2424324 (-) 429 WP_268568341.1 hypothetical protein -
  ACK2T8_RS12395 - 2424324..2424641 (-) 318 WP_158672721.1 phage head closure protein -
  ACK2T8_RS12400 - 2424619..2424915 (-) 297 WP_268568342.1 head-tail connector protein -
  ACK2T8_RS12405 - 2424917..2426101 (-) 1185 WP_158672719.1 phage major capsid protein -
  ACK2T8_RS12410 - 2426113..2426715 (-) 603 WP_077996844.1 HK97 family phage prohead protease -
  ACK2T8_RS12415 - 2426651..2427910 (-) 1260 WP_268568343.1 phage portal protein -
  ACK2T8_RS12420 - 2427929..2429644 (-) 1716 WP_409475286.1 terminase large subunit -
  ACK2T8_RS12425 - 2429631..2429936 (-) 306 WP_409475287.1 phage terminase small subunit P27 family -
  ACK2T8_RS12430 - 2430075..2430416 (-) 342 WP_036658631.1 HNH endonuclease -
  ACK2T8_RS12435 - 2430403..2430717 (-) 315 WP_023484126.1 hypothetical protein -
  ACK2T8_RS12440 - 2430897..2431100 (+) 204 WP_036658634.1 type II toxin-antitoxin system HicA family toxin -
  ACK2T8_RS12445 - 2431131..2431547 (+) 417 WP_023484796.1 type II toxin-antitoxin system HicB family antitoxin -
  ACK2T8_RS12450 - 2431602..2431946 (-) 345 WP_036658635.1 YxeA family protein -
  ACK2T8_RS12455 - 2432048..2433271 (-) 1224 WP_024093891.1 IS256 family transposase -
  ACK2T8_RS12460 - 2433536..2433976 (-) 441 WP_036658716.1 ArpU family phage packaging/lysis transcriptional regulator -
  ACK2T8_RS12465 - 2434102..2434245 (-) 144 WP_155116353.1 hypothetical protein -
  ACK2T8_RS12470 - 2434242..2434487 (-) 246 WP_036658638.1 hypothetical protein -
  ACK2T8_RS12475 - 2434480..2434713 (-) 234 WP_036658640.1 hypothetical protein -
  ACK2T8_RS12480 - 2434765..2434947 (-) 183 WP_036658642.1 hypothetical protein -
  ACK2T8_RS12485 - 2434931..2435713 (-) 783 WP_023483250.1 DNA cytosine methyltransferase -
  ACK2T8_RS12490 - 2435730..2436035 (-) 306 WP_309556053.1 hypothetical protein -
  ACK2T8_RS12495 - 2436277..2436651 (-) 375 WP_036658646.1 RusA family crossover junction endodeoxyribonuclease -
  ACK2T8_RS12500 - 2436644..2436862 (-) 219 WP_036658649.1 hypothetical protein -
  ACK2T8_RS12505 - 2436981..2437802 (-) 822 WP_077584884.1 ATP-binding protein -
  ACK2T8_RS12510 - 2437693..2438475 (-) 783 WP_023483153.1 DnaD domain-containing protein -
  ACK2T8_RS12515 - 2438555..2438887 (-) 333 WP_036658651.1 hypothetical protein -
  ACK2T8_RS12520 - 2438884..2439273 (-) 390 WP_036658652.1 hypothetical protein -
  ACK2T8_RS12525 ssbA 2439282..2439686 (-) 405 WP_036658655.1 single-stranded DNA-binding protein Machinery gene
  ACK2T8_RS12530 - 2439676..2440314 (-) 639 WP_023485215.1 ERF family protein -
  ACK2T8_RS12535 - 2440317..2440865 (-) 549 WP_023485214.1 host-nuclease inhibitor Gam family protein -
  ACK2T8_RS12540 - 2440862..2441125 (-) 264 WP_036658656.1 hypothetical protein -
  ACK2T8_RS12545 - 2441225..2441365 (-) 141 WP_155116354.1 hypothetical protein -
  ACK2T8_RS12550 - 2441343..2441687 (-) 345 WP_036658659.1 hypothetical protein -
  ACK2T8_RS12555 - 2441774..2442286 (+) 513 WP_036658661.1 hypothetical protein -
  ACK2T8_RS12560 - 2442276..2442533 (-) 258 WP_036658663.1 hypothetical protein -
  ACK2T8_RS12565 - 2442526..2443101 (-) 576 WP_036658665.1 hypothetical protein -
  ACK2T8_RS12570 - 2443098..2443850 (-) 753 WP_023483806.1 phage antirepressor KilAC domain-containing protein -
  ACK2T8_RS12575 - 2443855..2444028 (-) 174 WP_155116304.1 hypothetical protein -
  ACK2T8_RS12580 - 2444012..2444242 (-) 231 WP_036658666.1 hypothetical protein -
  ACK2T8_RS12585 - 2444239..2444616 (-) 378 WP_036658668.1 hypothetical protein -
  ACK2T8_RS12590 - 2444623..2444826 (-) 204 WP_036658670.1 hypothetical protein -
  ACK2T8_RS12595 - 2444847..2445116 (-) 270 WP_036658672.1 hypothetical protein -
  ACK2T8_RS12600 - 2445131..2445322 (-) 192 WP_144029586.1 hypothetical protein -
  ACK2T8_RS12605 - 2445445..2445717 (-) 273 WP_311795378.1 helix-turn-helix transcriptional regulator -
  ACK2T8_RS12610 - 2445762..2446100 (+) 339 WP_036658676.1 helix-turn-helix domain-containing protein -
  ACK2T8_RS12615 - 2446102..2447784 (+) 1683 WP_023484366.1 recombinase family protein -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 15606.49 Da        Isoelectric Point: 5.9505

>NTDB_id=1080528 ACK2T8_RS12525 WP_036658655.1 2439282..2439686(-) (ssbA) [Paenibacillus larvae subsp. larvae strain B-3650]
MLNRVVLIGRLTRDPELRYTPSGVAVTKFTLAVDRPYMNQQTRERETDFINIVTWRQTAEACANHLRKGRLTAVEGRIQV
RNYENNEGRRIYVTEVVADNVRFLESSKNENKRPEDPFYDSSTPLDISDDELPF

Nucleotide


Download         Length: 405 bp        

>NTDB_id=1080528 ACK2T8_RS12525 WP_036658655.1 2439282..2439686(-) (ssbA) [Paenibacillus larvae subsp. larvae strain B-3650]
ATGCTGAATAGGGTTGTTTTAATCGGCAGACTGACGAGAGATCCGGAATTAAGATATACTCCATCCGGTGTAGCTGTCAC
CAAGTTTACCCTGGCGGTAGACCGTCCGTATATGAACCAGCAAACGCGTGAGCGAGAGACGGATTTCATTAATATCGTGA
CGTGGAGACAAACCGCGGAAGCCTGTGCCAATCACCTTCGCAAAGGCCGTTTGACGGCAGTAGAAGGAAGAATCCAGGTG
CGTAATTATGAGAACAATGAGGGCCGCAGAATCTATGTAACCGAGGTTGTGGCTGATAATGTGCGCTTTCTTGAATCAAG
TAAAAATGAAAATAAACGCCCTGAAGACCCTTTCTACGACTCTAGCACACCACTAGACATTTCAGATGATGAACTCCCCT
TCTAG

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

47.977

100

0.619
  ssb Latilactobacillus sakei subsp. sakei 23K

45.029

100

0.575
  ssbB Bacillus subtilis subsp. subtilis str. 168

54.286

78.358

0.425
  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.791

100

0.418
  ssbB/cilA Streptococcus pneumoniae TIGR4

41.045

100

0.41
  ssbB/cilA Streptococcus pneumoniae Rx1

40.299

100

0.403
  ssbB/cilA Streptococcus mitis NCTC 12261

40.299

100

0.403
  ssbB/cilA Streptococcus pneumoniae D39

40.299

100

0.403
  ssbB/cilA Streptococcus pneumoniae R6

40.299

100

0.403
  ssbB/cilA Streptococcus mitis SK321

40.299

100

0.403
  ssb Neisseria gonorrhoeae MS11

41.6

93.284

0.388
  ssbA Streptococcus mutans UA159

38.06

100

0.381

Multiple sequence alignment