Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACJ3Y0_RS10040 Genome accession   NZ_CP176471
Coordinates   2221663..2223381 (+) Length   572 a.a.
NCBI ID   WP_372383794.1    Uniprot ID   -
Organism   Vibrio sp. BS-M-Sm-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2216663..2228381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3Y0_RS10020 (ACJ3Y0_10020) pilM 2218877..2219902 (+) 1026 WP_285953307.1 type IV pilus assembly protein PilM Machinery gene
  ACJ3Y0_RS10025 (ACJ3Y0_10025) pilN 2219886..2220500 (+) 615 WP_372383793.1 PilN domain-containing protein Machinery gene
  ACJ3Y0_RS10030 (ACJ3Y0_10030) pilO 2220493..2221122 (+) 630 WP_146492434.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJ3Y0_RS10035 (ACJ3Y0_10035) pilP 2221109..2221627 (+) 519 WP_210446842.1 pilus assembly protein PilP Machinery gene
  ACJ3Y0_RS10040 (ACJ3Y0_10040) pilQ 2221663..2223381 (+) 1719 WP_372383794.1 type IV pilus secretin PilQ Machinery gene
  ACJ3Y0_RS10045 (ACJ3Y0_10045) aroK 2223558..2224076 (+) 519 WP_017061125.1 shikimate kinase AroK -
  ACJ3Y0_RS10050 (ACJ3Y0_10050) aroB 2224102..2225190 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -
  ACJ3Y0_RS10055 (ACJ3Y0_10055) - 2225270..2226787 (+) 1518 WP_372383795.1 SPOR domain-containing protein -
  ACJ3Y0_RS10060 (ACJ3Y0_10060) - 2226857..2227702 (+) 846 WP_017632098.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62300.14 Da        Isoelectric Point: 4.4135

>NTDB_id=1080249 ACJ3Y0_RS10040 WP_372383794.1 2221663..2223381(+) (pilQ) [Vibrio sp. BS-M-Sm-2]
MIKGISRLVSKALQGFAVSVMLVVSVLSYAESLPNKLENIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELINTQ
VDDDKLYLLDVKDFATLVEGIEVYKETPSTRLLATISNDYQYEYNLKGRFIEVVISKPVIDEVTKEKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDRNVILVAPKAELDLREQQALEK
SRLEEELGELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQ
IEARIVTVTEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSAITPYDDDSGSSVIDDYLNVNLGATSPNASSIAFQVAKL
GSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLD
LSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETIVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSE
LLIFVTPKVVIQ

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1080249 ACJ3Y0_RS10040 WP_372383794.1 2221663..2223381(+) (pilQ) [Vibrio sp. BS-M-Sm-2]
ATGATTAAAGGAATAAGTAGATTAGTAAGCAAGGCTCTACAAGGTTTTGCTGTGTCTGTGATGTTGGTTGTTAGCGTGCT
CAGCTATGCCGAGAGCTTACCTAATAAATTAGAGAATATTGATTTTAGAGTGAACAAGGATAAAGACGCCGTCATCATCA
TTGAGCTGGCGACCAGTACCGCTGTGGTGGATGTTCAACGTGCTCAGGAAGGGTTAAGTATCGAGTTAATCAATACTCAG
GTTGATGATGACAAGCTTTACCTACTGGATGTGAAAGACTTCGCCACCTTGGTTGAAGGTATTGAGGTCTACAAAGAGAC
ACCAAGCACTCGGCTTCTAGCGACGATTAGCAATGACTATCAGTATGAATATAATCTAAAGGGGCGCTTTATTGAGGTGG
TGATCAGCAAACCGGTTATTGATGAAGTGACCAAAGAAAAAAGCGTTCTAGAGAAAGAGGGCAAGCTGATATCGATTAAC
TTCCAAGATATCCCAGTACGAAATGTCCTGCAGTTGATTGCCGACTATAACGATTTCAATCTCGTGGTGTCTGATTCGGT
AGCCGGAAACCTGACGTTACGTTTAGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGCTTGG
ATAAGCGTGTTGATCGCAATGTTATCTTGGTCGCGCCGAAAGCGGAACTCGATCTTCGAGAGCAACAAGCGTTAGAGAAA
TCTCGCTTGGAAGAGGAGTTAGGGGAGCTTAAGTCGGAAATCATCAAGATTAACTTTGCTAAAGCCACCGACATTGCCGA
CATGATTGGCGGTGAAGGCGCGGTCAGTATGTTGTCCGATCGTGGCTCAATTACCATTGATGAGCGCACCAACTCACTAC
TCATTCGTGAGCTGGAAGAAAACATTGCCGTGATCCGAGGCATCATTGAATCGTTAGACATTCCAGTGAAGCAAGTACAG
ATAGAGGCGCGTATCGTCACTGTTACCGAGGGTAACCTGGATGAGCTTGGTGTGCGCTGGGGTGTTTCATCAACCAACGG
AAGCTTCACCGTTGGCGGTTCAATTGAAGGTAACCACCCATCAGCGATTACGCCGTATGACGACGATAGCGGCAGCAGCG
TGATTGATGACTATCTCAACGTAAATTTAGGAGCAACATCGCCGAACGCTTCGAGTATTGCGTTTCAGGTAGCCAAGTTG
GGTTCGGATACCTTGCTTGATCTTGAATTGTCAGCACTGCAACAAGAGTCGAAAGCTGAAATTATTTCGAGCCCACGCTT
AATCACCACCAATAAAAAGCCCGCCTATATTGAGCAAGGTACTGAAATTCCTTATTTAGAGTCCTCTTCAAGTGGCGCTA
CGTCGGTCGCGTTTAAGAAAGCCGTGTTGAGTCTTAAGGTGACACCGCAGATCACGCCCGACAATCGTTTGGTATTGGAT
TTGAGCGTGACTCAAGATAGACCAGGGCAAGTGGTAAAAACCGGAACCGGTGAGGCGGTCGCAATTGATACGCAAAGAAT
AGGTACGCAAGTGCTTGTTAATAATGGTGAAACTATCGTGCTTGGCGGGATATTTCAACACAGCGTGAGCAGTACGGTGG
ATAAAGTTCCTCTGTTAGGAGACCTGCCAGTTTTGGGTGCATTGTTCCGTCGCAGCTATGAAAATGTGGGTAAAAGTGAA
CTGCTTATTTTTGTCACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Bacterial type II and III secretion system protein (Secretin)

(412-570)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

77.178

100

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.178

100

0.774
  pilQ Vibrio campbellii strain DS40M4

80.699

95.105

0.767
  pilQ Legionella pneumophila strain Lp02

42.491

95.455

0.406
  pilQ Legionella pneumophila strain ERS1305867

42.491

95.455

0.406
  pilQ Pseudomonas aeruginosa PAK

40.455

99.825

0.404
  comQ Acinetobacter baylyi ADP1

39.146

98.252

0.385
  pilQ Acinetobacter baumannii D1279779

37.478

100

0.379
  pilQ Acinetobacter baumannii strain A118

37.478

100

0.379

Multiple sequence alignment