Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACJ3Y0_RS10025 Genome accession   NZ_CP176471
Coordinates   2219886..2220500 (+) Length   204 a.a.
NCBI ID   WP_372383793.1    Uniprot ID   -
Organism   Vibrio sp. BS-M-Sm-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2214886..2225500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ3Y0_RS10005 (ACJ3Y0_10005) - 2214981..2215160 (-) 180 WP_017096544.1 hypothetical protein -
  ACJ3Y0_RS10010 (ACJ3Y0_10010) oxyR 2215159..2216052 (+) 894 WP_372057601.1 DNA-binding transcriptional regulator OxyR -
  ACJ3Y0_RS10015 (ACJ3Y0_10015) - 2216164..2218725 (-) 2562 WP_372383792.1 penicillin-binding protein 1A -
  ACJ3Y0_RS10020 (ACJ3Y0_10020) pilM 2218877..2219902 (+) 1026 WP_285953307.1 type IV pilus assembly protein PilM Machinery gene
  ACJ3Y0_RS10025 (ACJ3Y0_10025) pilN 2219886..2220500 (+) 615 WP_372383793.1 PilN domain-containing protein Machinery gene
  ACJ3Y0_RS10030 (ACJ3Y0_10030) pilO 2220493..2221122 (+) 630 WP_146492434.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACJ3Y0_RS10035 (ACJ3Y0_10035) pilP 2221109..2221627 (+) 519 WP_210446842.1 pilus assembly protein PilP Machinery gene
  ACJ3Y0_RS10040 (ACJ3Y0_10040) pilQ 2221663..2223381 (+) 1719 WP_372383794.1 type IV pilus secretin PilQ Machinery gene
  ACJ3Y0_RS10045 (ACJ3Y0_10045) aroK 2223558..2224076 (+) 519 WP_017061125.1 shikimate kinase AroK -
  ACJ3Y0_RS10050 (ACJ3Y0_10050) aroB 2224102..2225190 (+) 1089 WP_017061124.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23351.74 Da        Isoelectric Point: 9.3212

>NTDB_id=1080246 ACJ3Y0_RS10025 WP_372383793.1 2219886..2220500(+) (pilN) [Vibrio sp. BS-M-Sm-2]
MLHQINLLPWRDEIRAQHKKRFVHLVILGVIIALGGQWAVGNYFHDQQAKQQARLNYLNQYIAELDRQIQSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSDSLKGVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLENTTAESTAASRKEKGAHHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1080246 ACJ3Y0_RS10025 WP_372383793.1 2219886..2220500(+) (pilN) [Vibrio sp. BS-M-Sm-2]
ATGTTGCATCAAATTAACCTGCTGCCTTGGCGTGATGAGATTCGTGCTCAGCATAAGAAGCGTTTTGTTCATCTGGTCAT
TCTCGGTGTCATCATTGCGCTTGGTGGGCAATGGGCGGTCGGTAACTATTTTCACGACCAACAAGCCAAGCAGCAAGCGC
GCCTTAACTATCTCAATCAATACATCGCTGAGCTCGACCGACAGATTCAGTCATTAAAAGTCGCCGAACAAGAGCACAAA
GCCATTCTCACTCGACTAGATGTGGTGGAGTCTTTACAGCTTGGCCGCAATAAGACCACCGACTTCATGAACCTAATGCC
GGAGCTGATTCCTGAGGGTGTATATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGTATCAGCGACA
GTACGGCTCGTCTCGCCACGATGTTGGATAACCTCGAGCGTTCAGATTCTCTGAAAGGGGTTGAGATGCACTCTATCGTT
CATAACCGCAAACGCTTCAATAAAGAGTTTCAGACCTTCAAGGTCTCTTTTGTGTTCAGCCCTGTGTCTTTAGAAAACAC
AACCGCAGAGAGCACTGCCGCGAGCAGAAAAGAGAAGGGGGCACATCATGGCTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

60.976

100

0.613
  pilN Vibrio campbellii strain DS40M4

63.441

91.176

0.578

Multiple sequence alignment