Detailed information NTG1077547

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ACJROX_RS19450
• Genome accession: NZ_CP174509
• Coordinates: 3758190..3758606 (-)
• Length: 138 a.a.
• NCBI ID: WP_408006856.1
• Uniprot ID: -
• Organism: Pseudalkalibacillus sp. A8
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3753190..3763606

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACJROX_RS19420 (ACJROX_19420)	holA	3753670..3754710 (-)	1041	WP_408006850.1	DNA polymerase III subunit delta	-
ACJROX_RS19425 (ACJROX_19425)	-	3755049..3755183 (+)	135	WP_408006851.1	YqzM family protein	-
ACJROX_RS19430 (ACJROX_19430)	-	3755439..3756269 (-)	831	WP_408006852.1	ComEC/Rec2 family competence protein	-
ACJROX_RS19435 (ACJROX_19435)	-	3756316..3757242 (-)	927	WP_408006853.1	ComEC/Rec2 family competence protein	-
ACJROX_RS19440 (ACJROX_19440)	-	3757242..3757553 (-)	312	WP_408006854.1	hypothetical protein	-
ACJROX_RS19445 (ACJROX_19445)	-	3757550..3758128 (-)	579	WP_408006855.1	ComE operon protein 2	-
ACJROX_RS19450 (ACJROX_19450)	comEA	3758190..3758606 (-)	417	WP_408006856.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACJROX_RS19455 (ACJROX_19455)	comER	3758884..3759711 (+)	828	WP_408006857.1	late competence protein ComER	-
ACJROX_RS19460 (ACJROX_19460)	-	3760151..3760900 (-)	750	WP_408006858.1	class I SAM-dependent DNA methyltransferase	-
ACJROX_RS19465 (ACJROX_19465)	rsfS	3760913..3761266 (-)	354	WP_408006859.1	ribosome silencing factor	-
ACJROX_RS19470 (ACJROX_19470)	yqeK	3761263..3761838 (-)	576	WP_408006860.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ACJROX_RS19475 (ACJROX_19475)	-	3761825..3762409 (-)	585	WP_408006861.1	nicotinate-nucleotide adenylyltransferase	-
ACJROX_RS19480 (ACJROX_19480)	yhbY	3762416..3762709 (-)	294	WP_408006862.1	ribosome assembly RNA-binding protein YhbY	-
ACJROX_RS19485 (ACJROX_19485)	aroE	3762712..3763548 (-)	837	WP_408006863.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 138 a.a.
• Molecular weight: 14870.75 Da
• Isoelectric Point: 4.2632

>NTDB_id=1077547 ACJROX_RS19450 WP_408006856.1 3758190..3758606(-) (comEA) [Pseudalkalibacillus sp. A8]
MVVDVKGAVRTPGVFEMKSGQRVNDVVQLAGGFSDDADLNSVNLAQVLVDEMVIYVARVGEEAPPVVTNEKEEGKIDINH
AERTELETIPGIGLSKAGAINAYREENGRFSSIDDLVNVPGIGEKTLEQLKEYITAAK

Nucleotide

• Length: 417 bp

>NTDB_id=1077547 ACJROX_RS19450 WP_408006856.1 3758190..3758606(-) (comEA) [Pseudalkalibacillus sp. A8]
TTGGTTGTTGATGTAAAAGGAGCTGTACGTACACCAGGCGTTTTTGAAATGAAATCAGGTCAGCGGGTGAATGATGTTGT
GCAGCTTGCTGGAGGTTTCAGTGACGATGCAGATCTTAATAGTGTAAACTTAGCGCAAGTCCTGGTCGATGAGATGGTCA
TATACGTCGCAAGAGTAGGAGAGGAAGCACCTCCTGTAGTTACCAATGAAAAAGAAGAGGGGAAGATTGACATCAACCAC
GCAGAGCGTACAGAACTGGAGACGATTCCTGGAATCGGGCTATCGAAAGCAGGAGCCATCAACGCTTATCGTGAGGAAAA
TGGACGGTTTTCTTCTATTGATGATCTAGTCAATGTTCCGGGTATAGGGGAAAAAACACTTGAACAATTAAAAGAATATA
TCACAGCAGCTAAATAG

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (2-56)

• Helix-hairpin-helix motif (HHH_3) (73-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	46.98	100	0.507
comEA	Bacillus subtilis subsp. subtilis str. 168	46.479	100	0.478
comEA	Lactococcus lactis subsp. cremoris KW2	44.218	100	0.471
comEA/celA/cilE	Streptococcus mitis SK321	45.139	100	0.471
comEA	Latilactobacillus sakei subsp. sakei 23K	39.752	100	0.464
comEA/celA/cilE	Streptococcus mitis NCTC 12261	45.39	100	0.464
comEA/celA/cilE	Streptococcus pneumoniae D39	44.681	100	0.457
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	44.681	100	0.457
comEA/celA/cilE	Streptococcus pneumoniae Rx1	44.681	100	0.457
comEA/celA/cilE	Streptococcus pneumoniae R6	44.681	100	0.457
comEA	Staphylococcus aureus N315	39.437	100	0.406
comEA	Staphylococcus aureus MW2	38.732	100	0.399