Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACIQTD_RS11415 Genome accession   NZ_CP173185
Coordinates   2332470..2333408 (-) Length   312 a.a.
NCBI ID   WP_270250640.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain MGEL24009     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2327470..2338408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIQTD_RS11375 - 2328359..2329201 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  ACIQTD_RS11380 - 2329198..2329635 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  ACIQTD_RS11385 comGG 2329716..2330000 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACIQTD_RS11390 comGF 2330039..2330485 (-) 447 WP_032948129.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACIQTD_RS11395 comGE 2330448..2330744 (-) 297 WP_017865157.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACIQTD_RS11400 comGD 2330716..2331147 (-) 432 WP_026138965.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACIQTD_RS11405 comGC 2331107..2331376 (-) 270 WP_023349160.1 competence type IV pilus major pilin ComGC Machinery gene
  ACIQTD_RS11410 comGB 2331503..2332576 (-) 1074 WP_270250711.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACIQTD_RS11415 comGA 2332470..2333408 (-) 939 WP_270250640.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35707.88 Da        Isoelectric Point: 5.9473

>NTDB_id=1070522 ACIQTD_RS11415 WP_270250640.1 2332470..2333408(-) (comGA) [Lactococcus lactis subsp. lactis strain MGEL24009]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1070522 ACIQTD_RS11415 WP_270250640.1 2332470..2333408(-) (comGA) [Lactococcus lactis subsp. lactis strain MGEL24009]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.244

100

0.772
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.923

100

0.519

Multiple sequence alignment