Detailed information NTG1070517

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACIQTD_RS11390
• Genome accession: NZ_CP173185
• Coordinates: 2330039..2330485 (-)
• Length: 148 a.a.
• NCBI ID: WP_032948129.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain MGEL24009
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2325039..2335485

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACIQTD_RS11355	-	2325156..2325533 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
ACIQTD_RS11360	-	2325738..2326604 (+)	867	WP_058218508.1	RluA family pseudouridine synthase	-
ACIQTD_RS11365	-	2326643..2327452 (-)	810	WP_014570791.1	metal ABC transporter permease	-
ACIQTD_RS11370	-	2327445..2328182 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
ACIQTD_RS11375	-	2328359..2329201 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
ACIQTD_RS11380	-	2329198..2329635 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
ACIQTD_RS11385	comGG	2329716..2330000 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
ACIQTD_RS11390	comGF	2330039..2330485 (-)	447	WP_032948129.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACIQTD_RS11395	comGE	2330448..2330744 (-)	297	WP_017865157.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACIQTD_RS11400	comGD	2330716..2331147 (-)	432	WP_026138965.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACIQTD_RS11405	comGC	2331107..2331376 (-)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
ACIQTD_RS11410	comGB	2331503..2332576 (-)	1074	WP_270250711.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACIQTD_RS11415	comGA	2332470..2333408 (-)	939	WP_270250640.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=1070517 ACIQTD_RS11390 WP_032948129.1 2330039..2330485(-) (comGF) [Lactococcus lactis subsp. lactis strain MGEL24009]
MERKLCNLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=1070517 ACIQTD_RS11390 WP_032948129.1 2330039..2330485(-) (comGF) [Lactococcus lactis subsp. lactis strain MGEL24009]
ATGGAAAGGAAATTATGCAACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTG
TATGTAACTAAAGACAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAGAGAACATTTATTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comYF	Streptococcus mutans UA140	47.518	95.27	0.453
comYF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	42.336	92.568	0.392
comGF/cglF	Streptococcus mitis NCTC 12261	42.336	92.568	0.392
comGF/cglF	Streptococcus pneumoniae Rx1	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae D39	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae R6	39.716	95.27	0.378
comGF/cglF	Streptococcus pneumoniae TIGR4	39.716	95.27	0.378