Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACG2XO_RS00775 Genome accession   NZ_CP172558
Coordinates   172860..173996 (-) Length   378 a.a.
NCBI ID   WP_410498944.1    Uniprot ID   -
Organism   Chitinibacter sp. S2-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 167860..178996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACG2XO_RS00755 (ACG2XO_00755) - 168032..169201 (-) 1170 WP_410498940.1 NAD(P)/FAD-dependent oxidoreductase -
  ACG2XO_RS00760 (ACG2XO_00760) - 169381..170178 (+) 798 WP_410498941.1 Cof-type HAD-IIB family hydrolase -
  ACG2XO_RS00765 (ACG2XO_00765) - 170197..170730 (+) 534 WP_410498942.1 glutathione peroxidase -
  ACG2XO_RS00770 (ACG2XO_00770) - 170740..172854 (-) 2115 WP_410498943.1 FimV family protein -
  ACG2XO_RS00775 (ACG2XO_00775) pilU 172860..173996 (-) 1137 WP_410498944.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACG2XO_RS00780 (ACG2XO_00780) pilT 174014..175057 (-) 1044 WP_410498945.1 type IV pilus twitching motility protein PilT Machinery gene
  ACG2XO_RS00785 (ACG2XO_00785) - 175134..175850 (+) 717 WP_410498946.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACG2XO_RS00790 (ACG2XO_00790) proC 175850..176668 (+) 819 WP_410498947.1 pyrroline-5-carboxylate reductase -
  ACG2XO_RS00795 (ACG2XO_00795) - 176680..177264 (+) 585 WP_410498948.1 YggT family protein -
  ACG2XO_RS00800 (ACG2XO_00800) - 177268..177552 (+) 285 WP_410498949.1 DUF167 domain-containing protein -
  ACG2XO_RS00805 (ACG2XO_00805) - 177554..177991 (-) 438 WP_410498950.1 FxsA family protein -
  ACG2XO_RS00810 (ACG2XO_00810) cutA 177990..178328 (+) 339 WP_410498951.1 divalent-cation tolerance protein CutA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42447.57 Da        Isoelectric Point: 6.0550

>NTDB_id=1068019 ACG2XO_RS00775 WP_410498944.1 172860..173996(-) (pilU) [Chitinibacter sp. S2-10]
MEKEQAAKFMHDLLRHMRGKNASDLFITVDFPPAMKIDGRVTPVSNQVLSAQHTKELARAIMNDRQAEDFEAHKECNFAI
SPGNMGRFRVNAFMQQGRVGMVLRTINSDIPKLAELNLPPVLEDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNENAYD
HIITIEDPIEYVHEHKNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKAFVSQRLVPHASGKGRIAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEDGQITYEDALRNADSVNDLRLQIKLNSSDAKQRDVLSGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1068019 ACG2XO_RS00775 WP_410498944.1 172860..173996(-) (pilU) [Chitinibacter sp. S2-10]
ATGGAAAAAGAACAGGCTGCCAAATTTATGCATGACTTGCTACGCCATATGCGCGGCAAGAATGCATCCGATTTATTTAT
TACCGTTGATTTTCCTCCGGCAATGAAAATTGATGGGCGGGTGACGCCTGTTTCGAATCAAGTATTAAGTGCACAACACA
CCAAAGAGTTGGCGCGAGCCATCATGAATGATCGCCAGGCCGAAGACTTTGAAGCACACAAGGAATGTAACTTCGCGATC
AGTCCCGGCAATATGGGGCGTTTCCGGGTGAATGCCTTTATGCAGCAAGGTCGGGTTGGGATGGTGCTGCGGACAATTAA
TTCAGATATTCCCAAGCTGGCTGAATTGAATTTGCCTCCCGTTTTGGAAGATATTGCGATGACCAAACGTGGTTTGGTTA
TCTTTGTTGGCGGTACAGGGTCAGGTAAATCGACCTCGCTGGCTGCAATGATCGGTCATCGCAATGAAAATGCGTATGAC
CATATCATTACGATTGAAGACCCGATCGAATACGTGCATGAGCATAAAAACTCGATCATTACCCAGCGGGAAGTCGGGGT
CGATACCGATTCATGGATGGCTGCGCTGAAAAATACATTGCGCCAAGCGCCGGATGTGATTTTGATCGGTGAAATCCGGG
ATCGCGAGACGATGGATTACGCGATTGCTTTTGCCGAAACCGGCCACTTGTGTATGGCAACCTTGCACGCGAACTCATCG
AATCAGGCTCTGGACCGGATTATCAACTTTTTTCCCGAAGAGCGTCGTCAACAATTGTTGATGGATTTGTCTCTCAATTT
GAAAGCTTTTGTCTCGCAACGGCTGGTACCGCATGCCTCGGGCAAGGGACGGATTGCTGCGGTTGAAGTGATGCTCAATT
CCCCGCTGATTTCAGAACTGATTTTCAAGGGCGAAGTGCATGAAATCAAAGAGATTATGAAAAAATCTCGAGAGTTGGGG
ATGCAAACATTCGATCAATCCTTGTTTGATCTGTTTGAAGACGGTCAGATTACTTATGAAGATGCTTTGCGCAATGCTGA
TTCGGTCAATGATTTGCGTTTGCAGATCAAGCTAAATAGTTCTGATGCCAAGCAACGCGACGTGCTATCCGGGCTGGATC
ATCTGGATATTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.506

92.063

0.585
  pilU Acinetobacter baylyi ADP1

58.217

94.974

0.553
  pilU Vibrio cholerae strain A1552

53.521

93.915

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.866

88.624

0.415
  pilT Acinetobacter baylyi ADP1

43.425

86.508

0.376
  pilT Acinetobacter baumannii D1279779

42.991

84.921

0.365
  pilT Acinetobacter nosocomialis M2

42.991

84.921

0.365
  pilT Acinetobacter baumannii strain A118

42.991

84.921

0.365
  pilT Pseudomonas aeruginosa PAK

41.194

88.624

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.896

88.624

0.362

Multiple sequence alignment