Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   ACEY2B_RS00875 Genome accession   NZ_CP169315
Coordinates   179291..179674 (+) Length   127 a.a.
NCBI ID   WP_015427166.1    Uniprot ID   -
Organism   Lactococcus lactis strain IFLLLBEK1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 174291..184674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY2B_RS00860 (ACEY2B_00860) - 176146..177102 (+) 957 WP_052312061.1 hypothetical protein -
  ACEY2B_RS00865 (ACEY2B_00865) comGA 177372..178310 (+) 939 WP_015427168.1 competence type IV pilus ATPase ComGA Machinery gene
  ACEY2B_RS00870 (ACEY2B_00870) comGB 178204..179277 (+) 1074 WP_080619357.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACEY2B_RS00875 (ACEY2B_00875) comGC 179291..179674 (+) 384 WP_015427166.1 competence type IV pilus major pilin ComGC Machinery gene
  ACEY2B_RS00880 (ACEY2B_00880) comGD 179634..180065 (+) 432 WP_015427165.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACEY2B_RS00885 (ACEY2B_00885) comGE 180037..180333 (+) 297 WP_015427164.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACEY2B_RS00890 (ACEY2B_00890) comGF 180296..180742 (+) 447 WP_042230641.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACEY2B_RS00895 (ACEY2B_00895) comGG 180790..181065 (+) 276 WP_374788292.1 hypothetical protein Machinery gene
  ACEY2B_RS00900 (ACEY2B_00900) - 181147..181584 (+) 438 WP_136122555.1 zinc-dependent MarR family transcriptional regulator -
  ACEY2B_RS00905 (ACEY2B_00905) - 181581..182423 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  ACEY2B_RS00910 (ACEY2B_00910) - 182600..183337 (+) 738 WP_015427159.1 metal ABC transporter ATP-binding protein -
  ACEY2B_RS00915 (ACEY2B_00915) - 183330..184139 (+) 810 WP_015427158.1 metal ABC transporter permease -

Sequence


Protein


Download         Length: 127 a.a.        Molecular weight: 14898.48 Da        Isoelectric Point: 8.7864

>NTDB_id=1050458 ACEY2B_RS00875 WP_015427166.1 179291..179674(+) (comGC) [Lactococcus lactis strain IFLLLBEK1]
MERTKKFILKKIQKIFLFVKICEKKYRQKELKAFTLIEMLIVLAIISILILLFVPNLIKEKSQVQKTGEAAVVKVVENQA
QLYELDHDDEKPTLSELLSAGMITQKQISAYDNYYDQNKNEERNFND

Nucleotide


Download         Length: 384 bp        

>NTDB_id=1050458 ACEY2B_RS00875 WP_015427166.1 179291..179674(+) (comGC) [Lactococcus lactis strain IFLLLBEK1]
ATGGAAAGAACAAAGAAGTTTATTTTAAAGAAAATACAAAAAATATTTTTATTCGTTAAAATTTGTGAAAAAAAATACCG
TCAGAAAGAGCTAAAAGCTTTTACTTTGATAGAGATGTTAATTGTACTAGCTATTATTAGTATTTTGATATTACTATTTG
TTCCAAATTTAATTAAAGAAAAATCACAAGTTCAAAAAACTGGAGAAGCGGCAGTTGTGAAAGTAGTAGAAAATCAAGCC
CAACTTTATGAATTAGATCATGATGATGAGAAGCCGACTCTGTCAGAATTGCTCAGCGCTGGGATGATTACTCAAAAACA
AATTTCTGCTTACGATAATTACTATGATCAGAATAAAAATGAAGAACGAAATTTTAATGACTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Lactococcus lactis subsp. cremoris KW2

80.172

91.339

0.732

  comYC Streptococcus mutans UA159

55.446

79.528

0.441

  comYC Streptococcus mutans UA140

55.446

79.528

0.441

  comYC Streptococcus gordonii str. Challis substr. CH1

56.566

77.953

0.441

  comGC/cglC Streptococcus mitis SK321

54.455

79.528

0.433

  comGC/cglC Streptococcus pneumoniae TIGR4

52.475

79.528

0.417

  comGC/cglC Streptococcus pneumoniae Rx1

52.475

79.528

0.417

  comGC/cglC Streptococcus pneumoniae D39

52.475

79.528

0.417

  comGC/cglC Streptococcus pneumoniae R6

52.475

79.528

0.417

  comGC/cglC Streptococcus mitis NCTC 12261

56.18

70.079

0.394

  comYC Streptococcus suis isolate S10

56.818

69.291

0.394


Multiple sequence alignment