Detailed information NTG1050461

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ACEY2B_RS00890
• Genome accession: NZ_CP169315
• Coordinates: 180296..180742 (+)
• Length: 148 a.a.
• NCBI ID: WP_042230641.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain IFLLLBEK1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 175296..185742

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACEY2B_RS00860 (ACEY2B_00860)	-	176146..177102 (+)	957	WP_052312061.1	hypothetical protein	-
ACEY2B_RS00865 (ACEY2B_00865)	comGA	177372..178310 (+)	939	WP_015427168.1	competence type IV pilus ATPase ComGA	Machinery gene
ACEY2B_RS00870 (ACEY2B_00870)	comGB	178204..179277 (+)	1074	WP_080619357.1	competence type IV pilus assembly protein ComGB	Machinery gene
ACEY2B_RS00875 (ACEY2B_00875)	comGC	179291..179674 (+)	384	WP_015427166.1	competence type IV pilus major pilin ComGC	Machinery gene
ACEY2B_RS00880 (ACEY2B_00880)	comGD	179634..180065 (+)	432	WP_015427165.1	competence type IV pilus minor pilin ComGD	Machinery gene
ACEY2B_RS00885 (ACEY2B_00885)	comGE	180037..180333 (+)	297	WP_015427164.1	competence type IV pilus minor pilin ComGE	Machinery gene
ACEY2B_RS00890 (ACEY2B_00890)	comGF	180296..180742 (+)	447	WP_042230641.1	competence type IV pilus minor pilin ComGF	Machinery gene
ACEY2B_RS00895 (ACEY2B_00895)	comGG	180790..181065 (+)	276	WP_374788292.1	hypothetical protein	Machinery gene
ACEY2B_RS00900 (ACEY2B_00900)	-	181147..181584 (+)	438	WP_136122555.1	zinc-dependent MarR family transcriptional regulator	-
ACEY2B_RS00905 (ACEY2B_00905)	-	181581..182423 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
ACEY2B_RS00910 (ACEY2B_00910)	-	182600..183337 (+)	738	WP_015427159.1	metal ABC transporter ATP-binding protein	-
ACEY2B_RS00915 (ACEY2B_00915)	-	183330..184139 (+)	810	WP_015427158.1	metal ABC transporter permease	-
ACEY2B_RS00920 (ACEY2B_00920)	-	184177..185043 (-)	867	WP_042230639.1	RluA family pseudouridine synthase	-
ACEY2B_RS00925 (ACEY2B_00925)	-	185248..185625 (+)	378	WP_374788133.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17169.94 Da
• Isoelectric Point: 8.2376

>NTDB_id=1050461 ACEY2B_RS00890 WP_042230641.1 180296..180742(+) (comGF) [Lactococcus lactis strain IFLLLBEK1]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVEISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=1050461 ACEY2B_RS00890 WP_042230641.1 180296..180742(+) (comGF) [Lactococcus lactis strain IFLLLBEK1]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTTATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGAGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATCTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (46-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385