Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACEY2B_RS00865 Genome accession   NZ_CP169315
Coordinates   177372..178310 (+) Length   312 a.a.
NCBI ID   WP_015427168.1    Uniprot ID   -
Organism   Lactococcus lactis strain IFLLLBEK1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 172372..183310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY2B_RS00860 (ACEY2B_00860) - 176146..177102 (+) 957 WP_052312061.1 hypothetical protein -
  ACEY2B_RS00865 (ACEY2B_00865) comGA 177372..178310 (+) 939 WP_015427168.1 competence type IV pilus ATPase ComGA Machinery gene
  ACEY2B_RS00870 (ACEY2B_00870) comGB 178204..179277 (+) 1074 WP_080619357.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACEY2B_RS00875 (ACEY2B_00875) comGC 179291..179674 (+) 384 WP_015427166.1 competence type IV pilus major pilin ComGC Machinery gene
  ACEY2B_RS00880 (ACEY2B_00880) comGD 179634..180065 (+) 432 WP_015427165.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACEY2B_RS00885 (ACEY2B_00885) comGE 180037..180333 (+) 297 WP_015427164.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACEY2B_RS00890 (ACEY2B_00890) comGF 180296..180742 (+) 447 WP_042230641.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACEY2B_RS00895 (ACEY2B_00895) comGG 180790..181065 (+) 276 WP_374788292.1 hypothetical protein Machinery gene
  ACEY2B_RS00900 (ACEY2B_00900) - 181147..181584 (+) 438 WP_136122555.1 zinc-dependent MarR family transcriptional regulator -
  ACEY2B_RS00905 (ACEY2B_00905) - 181581..182423 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35631.91 Da        Isoelectric Point: 6.4009

>NTDB_id=1050456 ACEY2B_RS00865 WP_015427168.1 177372..178310(+) (comGA) [Lactococcus lactis strain IFLLLBEK1]
MIPKMAKELIQRAIDRGASDIYLIASKEKYKLYFRQMTARVLIEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHAASVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1050456 ACEY2B_RS00865 WP_015427168.1 177372..178310(+) (comGA) [Lactococcus lactis strain IFLLLBEK1]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGATAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGCAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCGCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAGAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.564

100

0.776
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

55.305

99.679

0.551
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.019

99.679

0.538
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.055

99.679

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.055

99.679

0.529
  comYA Streptococcus mutans UA140

52.564

100

0.526
  comYA Streptococcus mutans UA159

52.564

100

0.526
  comYA Streptococcus gordonii str. Challis substr. CH1

52.077

100

0.522

Multiple sequence alignment