Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEJ41_RS01585 Genome accession   NZ_CP169056
Coordinates   339487..340620 (-) Length   377 a.a.
NCBI ID   WP_374013054.1    Uniprot ID   -
Organism   Pseudoxanthomonas koreensis strain TDY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334487..345620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEJ41_RS01560 (ACEJ41_01560) - 334575..334964 (-) 390 WP_374013049.1 phasin family protein -
  ACEJ41_RS01565 (ACEJ41_01565) queD 335171..335524 (-) 354 WP_374013050.1 6-carboxytetrahydropterin synthase QueD -
  ACEJ41_RS01570 (ACEJ41_01570) - 335733..337115 (-) 1383 WP_374013051.1 DEAD/DEAH box helicase -
  ACEJ41_RS01575 (ACEJ41_01575) - 337607..338596 (+) 990 WP_374013052.1 fumarylacetoacetate hydrolase family protein -
  ACEJ41_RS01580 (ACEJ41_01580) maiA 338695..339375 (+) 681 WP_374013053.1 maleylacetoacetate isomerase -
  ACEJ41_RS01585 (ACEJ41_01585) pilU 339487..340620 (-) 1134 WP_374013054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEJ41_RS01590 (ACEJ41_01590) - 340721..341116 (+) 396 WP_374014035.1 DUF4398 domain-containing protein -
  ACEJ41_RS01595 (ACEJ41_01595) - 341113..342012 (+) 900 WP_374013055.1 hypothetical protein -
  ACEJ41_RS01600 (ACEJ41_01600) - 342145..342360 (-) 216 WP_374013056.1 YdcH family protein -
  ACEJ41_RS01605 (ACEJ41_01605) - 342877..344253 (+) 1377 WP_374013057.1 pyridoxal-phosphate dependent enzyme -
  ACEJ41_RS01610 (ACEJ41_01610) - 344648..345187 (-) 540 WP_374013058.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41677.13 Da        Isoelectric Point: 6.3728

>NTDB_id=1049589 ACEJ41_RS01585 WP_374013054.1 339487..340620(-) (pilU) [Pseudoxanthomonas koreensis strain TDY-1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLEMNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSAIPSIEELNLPQVLKDIIMTPRGLVLLVGSTGSGKSTALASMIDHRNSNITGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNILMNLALNLRAVVSLRLVKGMDERRLPASEVLINTPMIRDLLRRGQVHEIKQAMEGSLEEGMQTFDQCL
FRMVKQGQIQQEEALRAADSRDGLALKFRLSEGAEGEHDPYADINGPSSPTITHGFG

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1049589 ACEJ41_RS01585 WP_374013054.1 339487..340620(-) (pilU) [Pseudoxanthomonas koreensis strain TDY-1]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAGAAAAATGCCTCGGACATGTTCCTGACCACCGGGGCTCCGGTCTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTGGGCAATACCGGCCTGCCTCCGGGCATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGATGAACATGGCCATCGCCCTGGCCGATGCCGGGCGC
TTCCGGGTCAACGTGTTCAAGCAGCGCGGCGAGGTAGGCATGGTGATCCGCGCCATCCGCAGCGCGATCCCCTCCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGCTGGTCGGCTCCACCGGCT
CGGGCAAGTCCACCGCGCTGGCCTCGATGATCGACCACCGCAACAGCAACATCACCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAATCGATCGTCAACCAGCGCGAGGTGGGCCTGGATACGCACGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACCACGATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGTGCGCACAAGAACATCCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTGTCGCTGCG
CCTGGTCAAGGGCATGGACGAGCGCCGCCTGCCGGCCAGCGAGGTGCTGATCAACACCCCGATGATCCGCGACCTGCTGC
GCCGCGGGCAGGTGCACGAGATCAAGCAGGCGATGGAGGGGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGCAGGGCCAGATCCAGCAGGAGGAGGCGCTGCGCGCAGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGCCGAAGGCGAGCACGACCCCTACGCCGACATCAACGGCCCGTCCTCGCCCACCATCACCC
ACGGTTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-249)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.225

0.488
  pilU Acinetobacter baylyi ADP1

48.876

94.43

0.462
  pilU Vibrio cholerae strain A1552

47.458

93.899

0.446
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.455

100

0.44
  pilT Pseudomonas aeruginosa PAK

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Legionella pneumophila strain Lp02

40.533

89.655

0.363
  pilT Legionella pneumophila strain ERS1305867

40.533

89.655

0.363
  pilT Acinetobacter baylyi ADP1

40

90.186

0.361

Multiple sequence alignment