Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QUF32_RS13290 Genome accession   NZ_AP028128
Coordinates   2830234..2830650 (+) Length   138 a.a.
NCBI ID   WP_143683926.1    Uniprot ID   -
Organism   Vibrio fluvialis strain IDH05335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825234..2835650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF32_RS13265 (KKIDH5335_25450) dsbC 2826056..2826820 (-) 765 WP_143683923.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  QUF32_RS13270 (KKIDH5335_25460) xerD 2826837..2827745 (-) 909 WP_047459088.1 site-specific tyrosine recombinase XerD -
  QUF32_RS13275 (KKIDH5335_25470) fldB 2827886..2828407 (+) 522 WP_143683924.1 flavodoxin FldB -
  QUF32_RS13280 (KKIDH5335_25480) ampD 2828412..2828963 (-) 552 WP_143683925.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUF32_RS13285 (KKIDH5335_25490) nadC 2829116..2830003 (+) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUF32_RS13290 (KKIDH5335_25500) pilA 2830234..2830650 (+) 417 WP_143683926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QUF32_RS13295 (KKIDH5335_25510) pilB 2830657..2832345 (+) 1689 WP_143683927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUF32_RS13300 (KKIDH5335_25520) pilC 2832381..2833607 (+) 1227 WP_143683928.1 type II secretion system F family protein Machinery gene
  QUF32_RS13305 (KKIDH5335_25530) pilD 2833659..2834528 (+) 870 WP_143683929.1 A24 family peptidase Machinery gene
  QUF32_RS13310 (KKIDH5335_25540) coaE 2834531..2835139 (+) 609 WP_143683930.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14293.38 Da        Isoelectric Point: 6.2128

>NTDB_id=103949 QUF32_RS13290 WP_143683926.1 2830234..2830650(+) (pilA) [Vibrio fluvialis strain IDH05335]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKNYVSKSELASGLATLKSLITPAELYIQETGSLSGASASAFGVVS
GGVALGEITVPTASKLTFTFNDSSINGSTIDYDRSSGGWVCKVTLASGITLDIPKSCQ

Nucleotide


Download         Length: 417 bp        

>NTDB_id=103949 QUF32_RS13290 WP_143683926.1 2830234..2830650(+) (pilA) [Vibrio fluvialis strain IDH05335]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGGGTCCT
GTCGGCGATTGCCGTCCCAGCCTATAAAAATTATGTCTCTAAAAGTGAACTGGCGAGTGGACTAGCCACTCTCAAATCAT
TGATAACGCCTGCCGAATTATACATCCAAGAAACAGGTTCATTGAGCGGCGCATCAGCTTCTGCTTTTGGTGTTGTTAGT
GGCGGAGTTGCACTCGGGGAAATTACAGTTCCAACAGCAAGTAAACTTACATTTACGTTCAATGACAGCTCAATTAATGG
CAGTACGATTGACTATGACCGCTCTAGTGGTGGATGGGTTTGTAAAGTAACCTTAGCTTCGGGAATTACTTTAGATATAC
CAAAGAGTTGTCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

52.555

99.275

0.522
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.105

100

0.464
  pilA Vibrio cholerae strain A1552

42.105

100

0.464
  pilA Vibrio cholerae C6706

42.105

100

0.464
  pilA Pseudomonas aeruginosa PAK

37.333

100

0.406
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.045

97.101

0.399
  pilA Acinetobacter baumannii strain A118

38.194

100

0.399
  pilA/pilA1 Eikenella corrodens VA1

35.762

100

0.391

Multiple sequence alignment