Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QUF32_RS13295 Genome accession   NZ_AP028128
Coordinates   2830657..2832345 (+) Length   562 a.a.
NCBI ID   WP_143683927.1    Uniprot ID   -
Organism   Vibrio fluvialis strain IDH05335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825657..2837345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF32_RS13265 (KKIDH5335_25450) dsbC 2826056..2826820 (-) 765 WP_143683923.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  QUF32_RS13270 (KKIDH5335_25460) xerD 2826837..2827745 (-) 909 WP_047459088.1 site-specific tyrosine recombinase XerD -
  QUF32_RS13275 (KKIDH5335_25470) fldB 2827886..2828407 (+) 522 WP_143683924.1 flavodoxin FldB -
  QUF32_RS13280 (KKIDH5335_25480) ampD 2828412..2828963 (-) 552 WP_143683925.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUF32_RS13285 (KKIDH5335_25490) nadC 2829116..2830003 (+) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUF32_RS13290 (KKIDH5335_25500) pilA 2830234..2830650 (+) 417 WP_143683926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QUF32_RS13295 (KKIDH5335_25510) pilB 2830657..2832345 (+) 1689 WP_143683927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUF32_RS13300 (KKIDH5335_25520) pilC 2832381..2833607 (+) 1227 WP_143683928.1 type II secretion system F family protein Machinery gene
  QUF32_RS13305 (KKIDH5335_25530) pilD 2833659..2834528 (+) 870 WP_143683929.1 A24 family peptidase Machinery gene
  QUF32_RS13310 (KKIDH5335_25540) coaE 2834531..2835139 (+) 609 WP_143683930.1 dephospho-CoA kinase -
  QUF32_RS13315 (KKIDH5335_25550) zapD 2835172..2835912 (+) 741 WP_020332196.1 cell division protein ZapD -
  QUF32_RS13320 (KKIDH5335_25560) yacG 2835967..2836164 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62041.95 Da        Isoelectric Point: 6.2647

>NTDB_id=103950 QUF32_RS13295 WP_143683927.1 2830657..2832345(+) (pilB) [Vibrio fluvialis strain IDH05335]
MLTNLIAILRQAELISPTQEQAVMDHIHASGTNVPEALIDLAIFHHHDLTEHLSQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQVLTLAVSDPTHLQVEDEFRFATGLQIELVLADHKALQAAIRRLYGRAIASHGTQGKEISQDELAS
LVQVSDEEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHFSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=103950 QUF32_RS13295 WP_143683927.1 2830657..2832345(+) (pilB) [Vibrio fluvialis strain IDH05335]
ATGCTTACCAACCTTATCGCCATTCTACGTCAGGCTGAGTTGATCAGCCCAACGCAGGAACAGGCCGTGATGGATCATAT
TCACGCTTCCGGAACCAATGTGCCGGAAGCGTTGATCGATTTGGCGATATTCCATCACCACGATCTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACTCGTTTTGACTTCGCGCCGCTTTGCCAGCAGTTGGGGCTGCGAGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAACAGCAACGGTCAGGTACTGACTCTTGCGGTATCCGATCCTACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAATAGAACTGGTGTTGGCTGATCATAAGGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCGCGTCACATGGCACACAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGAAGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCATTTTGAGCCCTATGAATCCTTTTATCGCATTCGCT
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCCAGCCATTTAAGCCGCCGACTGGCTGCGCGGATTAAGATT
TTGTCGAAACTCGATATCGCTGAGCGACGTTTACCGCAAGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTTAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GACGCCTTTGCCCTCACTGCAAAGTCGCCACATCACCCTCTCCGACACTGCAGCAACAATTTCACTTTAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGCTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.918

100

0.829
  pilB Vibrio campbellii strain DS40M4

72.064

100

0.721
  pilB Vibrio parahaemolyticus RIMD 2210633

71.174

100

0.712
  pilB Legionella pneumophila strain ERS1305867

52.14

91.459

0.477
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilB Acinetobacter baylyi ADP1

49.615

92.527

0.459
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.445
  pilF Thermus thermophilus HB27

37.544

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.818

97.865

0.37

Multiple sequence alignment