Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   QUF32_RS13300 Genome accession   NZ_AP028128
Coordinates   2832381..2833607 (+) Length   408 a.a.
NCBI ID   WP_143683928.1    Uniprot ID   -
Organism   Vibrio fluvialis strain IDH05335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2827381..2838607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUF32_RS13275 (KKIDH5335_25470) fldB 2827886..2828407 (+) 522 WP_143683924.1 flavodoxin FldB -
  QUF32_RS13280 (KKIDH5335_25480) ampD 2828412..2828963 (-) 552 WP_143683925.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QUF32_RS13285 (KKIDH5335_25490) nadC 2829116..2830003 (+) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QUF32_RS13290 (KKIDH5335_25500) pilA 2830234..2830650 (+) 417 WP_143683926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QUF32_RS13295 (KKIDH5335_25510) pilB 2830657..2832345 (+) 1689 WP_143683927.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QUF32_RS13300 (KKIDH5335_25520) pilC 2832381..2833607 (+) 1227 WP_143683928.1 type II secretion system F family protein Machinery gene
  QUF32_RS13305 (KKIDH5335_25530) pilD 2833659..2834528 (+) 870 WP_143683929.1 A24 family peptidase Machinery gene
  QUF32_RS13310 (KKIDH5335_25540) coaE 2834531..2835139 (+) 609 WP_143683930.1 dephospho-CoA kinase -
  QUF32_RS13315 (KKIDH5335_25550) zapD 2835172..2835912 (+) 741 WP_020332196.1 cell division protein ZapD -
  QUF32_RS13320 (KKIDH5335_25560) yacG 2835967..2836164 (+) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  QUF32_RS13325 - 2836262..2837466 (+) 1205 WP_286236231.1 IS3 family transposase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44700.94 Da        Isoelectric Point: 10.5318

>NTDB_id=103951 QUF32_RS13300 WP_143683928.1 2832381..2833607(+) (pilC) [Vibrio fluvialis strain IDH05335]
MKPTKGPELKNYRWKGVNSTGKKVAGQTLAISEIEVRDKLKEQHIQVKKIKKGSVSVLTRLTNRVKTKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKGVEAGTPISKAMRTASTHFDALYVDLIQTGEQSGNLAEVFERLATYRE
KSEQLRAKVIKALIYPSMVILVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQILKLSHGVQAYSAGAFIASLSLVFG
IKAARKKSFVVRIKTSQLSLRFPIIGGVLAKAAIAKFSRTLATSFSAGIPILASLKTTAKTAGNVHFETAIQDVHRHTAA
GMPMYIAMRNTQAFPEMVLQMVMIGEESGKLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGIVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=103951 QUF32_RS13300 WP_143683928.1 2832381..2833607(+) (pilC) [Vibrio fluvialis strain IDH05335]
ATGAAACCGACCAAAGGCCCTGAGTTAAAAAACTATCGCTGGAAAGGCGTCAACAGCACTGGCAAGAAAGTCGCGGGACA
GACACTGGCTATCAGTGAAATAGAAGTCCGCGACAAACTGAAAGAACAGCACATTCAGGTTAAGAAGATCAAAAAAGGCA
GCGTGTCAGTTCTGACTCGTCTCACCAATCGGGTCAAAACTAAAGACATCACCATCCTAACCCGCCAGCTTGCCACCATG
CTGACCACCGGAGTGCCGATTGTGCAGGCACTTAAATTGGTCGGCGATAATCATCGTAAAGCCGAGATGAAATCGATTCT
GGCGCAAATTACCAAAGGCGTCGAAGCCGGTACGCCGATTTCCAAAGCGATGCGTACTGCCAGCACTCACTTTGACGCGT
TGTACGTCGACTTAATTCAGACTGGCGAGCAGTCCGGCAATCTTGCCGAAGTCTTTGAACGCCTTGCCACCTATCGGGAA
AAAAGCGAGCAACTGCGCGCGAAAGTAATCAAAGCGCTGATCTATCCCAGCATGGTCATTCTGGTGGCACTCGGCGTCTC
CTATTTGATGCTGACGATGGTCATTCCAGAGTTCGAAAGTATGTTCAAGGGTTTTGGGGCTGAACTGCCTTGGTTTACTC
AGCAGATCCTCAAGTTATCGCATGGGGTACAAGCCTACAGTGCGGGGGCGTTCATCGCGTCATTGAGCTTGGTGTTTGGC
ATCAAAGCGGCGCGGAAAAAATCCTTCGTGGTCCGTATTAAAACCAGCCAGCTCAGCCTGCGTTTTCCCATCATTGGCGG
TGTGCTTGCCAAAGCCGCAATTGCCAAATTCAGCCGCACTCTGGCGACCAGCTTCAGTGCGGGAATTCCGATACTCGCCA
GCCTCAAAACCACCGCTAAAACGGCAGGTAACGTGCATTTTGAAACCGCCATTCAGGATGTTCACCGCCATACGGCAGCA
GGGATGCCGATGTATATTGCAATGCGCAACACACAAGCCTTTCCGGAAATGGTGTTGCAAATGGTGATGATCGGCGAGGA
GTCCGGCAAGCTCGACGACATGCTCAATAAAGTCGCCACCATCTATGAGTTTGAAGTCGACAACACCGTCGACAATCTGG
GCAAAATTCTTGAACCGCTGATTATCGTATTTTTGGGCATTGTGGTCGGTGGATTGGTGGTGGCGATGTACCTGCCAATC
TTTAACTTAATGAGTGTATTAGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

86.765

100

0.868
  pilC Vibrio campbellii strain DS40M4

72.346

99.265

0.718
  pilC Acinetobacter baumannii D1279779

41.667

100

0.417
  pilC Legionella pneumophila strain ERS1305867

41.103

97.794

0.402
  pilC Acinetobacter baylyi ADP1

41.31

97.304

0.402
  pilC Pseudomonas stutzeri DSM 10701

39.899

97.059

0.387
  pilG Neisseria gonorrhoeae MS11

39.599

97.794

0.387
  pilG Neisseria meningitidis 44/76-A

39.098

97.794

0.382

Multiple sequence alignment