Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACB088_RS17870 Genome accession   NZ_CP167121
Coordinates   3780091..3780522 (+) Length   143 a.a.
NCBI ID   WP_375037610.1    Uniprot ID   -
Organism   Acinetobacter sp. RW6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3775091..3785522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACB088_RS17850 (ACB088_17850) - 3776485..3777372 (+) 888 WP_035358234.1 metal-dependent hydrolase -
  ACB088_RS17855 (ACB088_17855) - 3777501..3778091 (+) 591 WP_003653797.1 LemA family protein -
  ACB088_RS17860 (ACB088_17860) - 3778116..3779195 (+) 1080 WP_375037608.1 TPM domain-containing protein -
  ACB088_RS17865 (ACB088_17865) - 3779189..3779749 (+) 561 WP_375037609.1 TPM domain-containing protein -
  ACB088_RS17870 (ACB088_17870) pilA 3780091..3780522 (+) 432 WP_375037610.1 pilin Machinery gene
  ACB088_RS17875 (ACB088_17875) - 3780579..3781889 (+) 1311 WP_375037611.1 O-antigen ligase family protein -
  ACB088_RS17880 (ACB088_17880) - 3781995..3783623 (+) 1629 WP_375037612.1 pilin glycosylation ligase domain-containing protein -
  ACB088_RS17885 (ACB088_17885) bfr 3783667..3784131 (-) 465 WP_375037613.1 bacterioferritin -
  ACB088_RS17890 (ACB088_17890) - 3784376..3784570 (-) 195 WP_004639703.1 bacterioferritin-associated ferredoxin -
  ACB088_RS17895 (ACB088_17895) - 3784792..3785175 (-) 384 WP_002116996.1 RidA family protein -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14643.68 Da        Isoelectric Point: 4.7062

>NTDB_id=1038311 ACB088_RS17870 WP_375037610.1 3780091..3780522(+) (pilA) [Acinetobacter sp. RW6]
MNAQKGFTLIELMIVVAIIGILAAIAIPQYSKYQAKSKLTASLSEISAGKTVAEERINNGEAVTLADLGLQATTSNCNIA
VTGFAAGSGDGTIECTVQNTPSQVTGAVMTWTRVGATGIWSCETTGITDTDNKTLAPKGCAAS

Nucleotide


Download         Length: 432 bp        

>NTDB_id=1038311 ACB088_RS17870 WP_375037610.1 3780091..3780522(+) (pilA) [Acinetobacter sp. RW6]
ATGAATGCACAAAAAGGTTTTACATTAATTGAACTCATGATCGTGGTTGCCATTATTGGTATTTTGGCGGCAATTGCGAT
TCCACAGTATAGTAAGTATCAAGCAAAATCTAAATTAACAGCAAGTTTGTCAGAGATTTCAGCAGGTAAAACTGTAGCTG
AAGAGCGAATTAATAATGGGGAGGCAGTTACCCTAGCTGATCTTGGCCTTCAGGCAACTACATCAAATTGTAATATTGCT
GTAACTGGTTTTGCAGCAGGTAGTGGTGATGGAACTATTGAATGTACAGTTCAAAATACCCCTTCTCAAGTTACAGGTGC
AGTAATGACTTGGACAAGAGTTGGTGCAACTGGCATTTGGAGCTGTGAAACTACAGGTATTACAGATACAGATAATAAAA
CACTTGCTCCTAAAGGATGTGCAGCATCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

47.586

100

0.483
  comP Acinetobacter baylyi ADP1

39.869

100

0.427
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.541

100

0.42
  pilA Vibrio cholerae strain A1552

40.541

100

0.42
  pilA Vibrio cholerae C6706

40.541

100

0.42
  pilA Pseudomonas aeruginosa PAK

38.71

100

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.312

89.51

0.406
  pilA Vibrio parahaemolyticus RIMD 2210633

44.8

87.413

0.392
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.732

99.301

0.385
  pilA2 Legionella pneumophila strain ERS1305867

42.857

88.112

0.378
  pilA2 Legionella pneumophila str. Paris

41.27

88.112

0.364

Multiple sequence alignment