Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACA097_RS17670 Genome accession   NZ_CP166971
Coordinates   3764456..3765676 (-) Length   406 a.a.
NCBI ID   WP_371364859.1    Uniprot ID   -
Organism   Pseudomonas sp. QL9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3764456..3793076 3764456..3765676 within 0


Gene organization within MGE regions


Location: 3764456..3793076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA097_RS17670 (ACA097_17665) pilC 3764456..3765676 (-) 1221 WP_371364859.1 type II secretion system F family protein Machinery gene
  ACA097_RS17675 (ACA097_17670) pilB 3765680..3767383 (-) 1704 WP_371364862.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACA097_RS17680 (ACA097_17675) comP 3767609..3768031 (+) 423 WP_371364865.1 pilin Machinery gene
  ACA097_RS17685 (ACA097_17680) - 3768079..3769167 (+) 1089 WP_371364867.1 hypothetical protein -
  ACA097_RS17695 (ACA097_17690) - 3769469..3770788 (+) 1320 WP_371364870.1 tyrosine-type recombinase/integrase -
  ACA097_RS17700 (ACA097_17695) - 3770785..3771603 (+) 819 WP_371364872.1 hypothetical protein -
  ACA097_RS17705 (ACA097_17700) - 3771695..3771928 (+) 234 WP_371364874.1 helix-turn-helix transcriptional regulator -
  ACA097_RS17710 (ACA097_17705) - 3771925..3772338 (+) 414 WP_371364877.1 helix-turn-helix domain-containing protein -
  ACA097_RS17715 (ACA097_17710) - 3772774..3773088 (+) 315 WP_371364879.1 hypothetical protein -
  ACA097_RS17720 (ACA097_17715) - 3773085..3773396 (+) 312 WP_371364881.1 hypothetical protein -
  ACA097_RS17725 (ACA097_17720) - 3773401..3774324 (+) 924 WP_371364884.1 toprim domain-containing protein -
  ACA097_RS17730 (ACA097_17725) - 3774321..3775865 (+) 1545 WP_371364888.1 DUF3631 domain-containing protein -
  ACA097_RS17735 (ACA097_17730) - 3775865..3776158 (+) 294 WP_371364890.1 hypothetical protein -
  ACA097_RS17740 (ACA097_17735) - 3776214..3777110 (+) 897 WP_371364893.1 hypothetical protein -
  ACA097_RS17745 (ACA097_17740) - 3777405..3778136 (-) 732 WP_371364896.1 HNH endonuclease -
  ACA097_RS17750 (ACA097_17745) - 3778136..3779470 (-) 1335 WP_371364899.1 AAA family ATPase -
  ACA097_RS17755 (ACA097_17750) - 3779633..3779866 (+) 234 WP_371364902.1 hypothetical protein -
  ACA097_RS17760 (ACA097_17755) - 3780169..3780621 (+) 453 WP_371364904.1 DUF1441 family protein -
  ACA097_RS17765 (ACA097_17760) - 3780766..3782178 (+) 1413 WP_371364906.1 HEPN domain-containing protein -
  ACA097_RS17770 (ACA097_17765) - 3783412..3787746 (+) 4335 WP_371364908.1 AAA domain-containing protein -
  ACA097_RS17775 (ACA097_17770) - 3787846..3788589 (+) 744 WP_371364910.1 hypothetical protein -
  ACA097_RS17780 (ACA097_17775) - 3789260..3790117 (-) 858 WP_371364913.1 HNH endonuclease -
  ACA097_RS17785 (ACA097_17780) - 3790557..3791795 (+) 1239 Protein_3492 transposase -
  ACA097_RS17790 (ACA097_17785) - 3792006..3793076 (+) 1071 WP_371364915.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44107.80 Da        Isoelectric Point: 9.8328

>NTDB_id=1036485 ACA097_RS17670 WP_371364859.1 3764456..3765676(-) (pilC) [Pseudomonas sp. QL9]
MAEKALKTSVFIFEGTDRKGGKIKGELSGTNAALVKAQLRKQGINPTKVRKKGISLLGKGKKIQPMDIALFTRQMATMMG
AGVPLLQAFDIIADGFDNPNMRKLVDDIKQEVASGNSLANSLRKKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVVVVAVIVSAILLIKVVPQFQEVFANFGAELPAFTLMVIHLSEILQKWWLIVLVALFAAAFSLK
QFHTRSEKFRDALDKASLKIPVIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVEKVKQDVSSGT
QLNFSMRTTGVFPNMAIQMTAIGEESGSLDAMLDKVASFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNAVR

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1036485 ACA097_RS17670 WP_371364859.1 3764456..3765676(-) (pilC) [Pseudomonas sp. QL9]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTTTTCATATTCGAAGGCACGGATCGCAAGGGCGGCAAAATAAAAGGGGAGCT
TTCGGGAACAAATGCTGCGCTAGTCAAAGCGCAACTTAGAAAGCAAGGCATAAATCCAACCAAAGTGCGCAAGAAGGGCA
TATCTTTGCTCGGTAAGGGCAAAAAAATCCAACCTATGGACATTGCGCTCTTCACCAGGCAAATGGCCACCATGATGGGT
GCAGGTGTCCCTTTGCTTCAAGCCTTCGATATAATTGCAGATGGCTTTGACAACCCTAACATGCGCAAACTGGTGGACGA
CATAAAGCAAGAGGTTGCTTCGGGCAATAGTCTTGCCAACTCTCTCCGAAAGAAACCGCAATATTTCGATGATCTTTATT
GCAATCTCGTAGACGCCGGAGAGCAGTCTGGCGCACTAGAAACACTACTTGACCGAGTTGCCACCTATAAAGAAAAAACA
GAGTCCCTTAAAGCAAAAATCAAAAAAGCAATGACCTATCCCATCGCAGTGGTAGTAGTTGCAGTCATCGTTTCTGCCAT
TTTGCTAATCAAGGTAGTACCTCAGTTCCAAGAAGTATTTGCCAATTTTGGCGCTGAATTACCCGCATTTACCTTGATGG
TGATCCATTTATCAGAGATCCTTCAAAAATGGTGGCTGATAGTCTTGGTTGCACTATTCGCAGCCGCATTTTCTCTAAAA
CAATTTCACACACGATCTGAGAAATTTAGAGACGCTCTCGACAAGGCCTCCTTAAAAATTCCAGTCATCGGAGGAATACT
TTACAAATCGGCCATTGCGAGATATGCCCGCACGCTTTCTACGACGTTTGCAGCAGGCGTCCCGCTAGTAGAAGCTCTCG
ACTCTGTTTCCGGAGCGACTGGAAACGTAGTATTCAGAAATGCAGTTGAAAAGGTAAAACAAGACGTTTCCAGCGGCACG
CAGTTGAACTTCTCAATGCGTACAACTGGAGTGTTTCCTAACATGGCTATTCAAATGACAGCCATCGGTGAGGAGTCCGG
CTCTCTCGATGCCATGCTAGACAAAGTTGCATCTTTTTACGAGGAAGAAGTCGACAATGCCGTTGACAATTTAACTACAC
TGATGGAACCAATGATTATGGCAGTATTAGGGGTGCTTGTAGGCGGACTTATTATTGCGATGTATCTCCCCATATTCCAA
TTGGGCAATGCAGTTCGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80.247

99.754

0.8
  pilC Acinetobacter baumannii D1279779

59.012

99.754

0.589
  pilC Acinetobacter baylyi ADP1

57.635

100

0.576
  pilC Legionella pneumophila strain ERS1305867

55.303

97.537

0.539
  pilG Neisseria meningitidis 44/76-A

45.522

99.015

0.451
  pilG Neisseria gonorrhoeae MS11

45.274

99.015

0.448
  pilC Vibrio cholerae strain A1552

41.646

98.768

0.411
  pilC Vibrio campbellii strain DS40M4

41.071

96.552

0.397
  pilC Thermus thermophilus HB27

37.656

98.768

0.372

Multiple sequence alignment