Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACA097_RS17675 Genome accession   NZ_CP166971
Coordinates   3765680..3767383 (-) Length   567 a.a.
NCBI ID   WP_371364862.1    Uniprot ID   -
Organism   Pseudomonas sp. QL9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3764456..3793076 3765680..3767383 within 0


Gene organization within MGE regions


Location: 3764456..3793076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACA097_RS17670 (ACA097_17665) pilC 3764456..3765676 (-) 1221 WP_371364859.1 type II secretion system F family protein Machinery gene
  ACA097_RS17675 (ACA097_17670) pilB 3765680..3767383 (-) 1704 WP_371364862.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACA097_RS17680 (ACA097_17675) comP 3767609..3768031 (+) 423 WP_371364865.1 pilin Machinery gene
  ACA097_RS17685 (ACA097_17680) - 3768079..3769167 (+) 1089 WP_371364867.1 hypothetical protein -
  ACA097_RS17695 (ACA097_17690) - 3769469..3770788 (+) 1320 WP_371364870.1 tyrosine-type recombinase/integrase -
  ACA097_RS17700 (ACA097_17695) - 3770785..3771603 (+) 819 WP_371364872.1 hypothetical protein -
  ACA097_RS17705 (ACA097_17700) - 3771695..3771928 (+) 234 WP_371364874.1 helix-turn-helix transcriptional regulator -
  ACA097_RS17710 (ACA097_17705) - 3771925..3772338 (+) 414 WP_371364877.1 helix-turn-helix domain-containing protein -
  ACA097_RS17715 (ACA097_17710) - 3772774..3773088 (+) 315 WP_371364879.1 hypothetical protein -
  ACA097_RS17720 (ACA097_17715) - 3773085..3773396 (+) 312 WP_371364881.1 hypothetical protein -
  ACA097_RS17725 (ACA097_17720) - 3773401..3774324 (+) 924 WP_371364884.1 toprim domain-containing protein -
  ACA097_RS17730 (ACA097_17725) - 3774321..3775865 (+) 1545 WP_371364888.1 DUF3631 domain-containing protein -
  ACA097_RS17735 (ACA097_17730) - 3775865..3776158 (+) 294 WP_371364890.1 hypothetical protein -
  ACA097_RS17740 (ACA097_17735) - 3776214..3777110 (+) 897 WP_371364893.1 hypothetical protein -
  ACA097_RS17745 (ACA097_17740) - 3777405..3778136 (-) 732 WP_371364896.1 HNH endonuclease -
  ACA097_RS17750 (ACA097_17745) - 3778136..3779470 (-) 1335 WP_371364899.1 AAA family ATPase -
  ACA097_RS17755 (ACA097_17750) - 3779633..3779866 (+) 234 WP_371364902.1 hypothetical protein -
  ACA097_RS17760 (ACA097_17755) - 3780169..3780621 (+) 453 WP_371364904.1 DUF1441 family protein -
  ACA097_RS17765 (ACA097_17760) - 3780766..3782178 (+) 1413 WP_371364906.1 HEPN domain-containing protein -
  ACA097_RS17770 (ACA097_17765) - 3783412..3787746 (+) 4335 WP_371364908.1 AAA domain-containing protein -
  ACA097_RS17775 (ACA097_17770) - 3787846..3788589 (+) 744 WP_371364910.1 hypothetical protein -
  ACA097_RS17780 (ACA097_17775) - 3789260..3790117 (-) 858 WP_371364913.1 HNH endonuclease -
  ACA097_RS17785 (ACA097_17780) - 3790557..3791795 (+) 1239 Protein_3492 transposase -
  ACA097_RS17790 (ACA097_17785) - 3792006..3793076 (+) 1071 WP_371364915.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62708.84 Da        Isoelectric Point: 5.4691

>NTDB_id=1036486 ACA097_RS17675 WP_371364862.1 3765680..3767383(-) (pilB) [Pseudomonas sp. QL9]
MNDQAVLTGLARQLVIAELLDEKSAQQAQQQAQRNKLPLVTYLVQNKLVKSRPLIELAAEQFGIAYFDLGSLDRESIPRD
LISERLVRQHRVLPLWRRGNKLFVGLSDPTNHQAITDVQFSTGLTTEAILVEDDKLGDAIEKLFESPTGGLDDLGEVDLE
GLDIESGNVERKEEAIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRLSAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDNQKQLYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGMDFSTALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSVNLIIAQRLARKLCSACKKEHEVPREALLHEGFP
EDRIGTFKLYSPVGCENCNGGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1036486 ACA097_RS17675 WP_371364862.1 3765680..3767383(-) (pilB) [Pseudomonas sp. QL9]
ATGAACGACCAAGCCGTACTTACCGGCCTCGCACGCCAACTGGTGATAGCTGAGCTGCTCGACGAAAAGAGCGCACAGCA
GGCTCAGCAACAAGCGCAACGTAACAAGCTTCCGCTGGTAACCTACCTGGTGCAAAACAAGCTGGTGAAAAGCCGACCTC
TGATTGAGCTTGCAGCAGAGCAATTCGGCATCGCCTACTTCGACCTTGGCAGCCTTGATCGAGAAAGCATTCCAAGGGAC
TTGATAAGCGAGAGACTGGTCCGCCAGCATCGAGTCCTCCCTTTGTGGCGGCGAGGCAACAAGCTGTTTGTAGGGCTTTC
CGATCCTACCAATCACCAAGCCATCACCGACGTACAATTCAGTACGGGCCTTACTACTGAGGCAATCCTGGTCGAGGATG
ACAAACTCGGGGACGCCATCGAGAAGCTTTTCGAGAGCCCGACTGGGGGACTGGATGACCTTGGCGAGGTCGACCTCGAA
GGACTTGATATCGAAAGTGGGAATGTTGAACGCAAAGAAGAGGCAATCGGTGGCGATGCCGACGATGCGCCGGTGGTGCG
ATTCGTCAACAAGATGCTGCTGGATGCTATTAAGGGCGGTTCATCAGATCTGCACTTCGAACCCTACGAGAAAATCTACC
GCGTGCGTTTTCGTACTGACGGCATGCTGCACGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTCTCTCCGCACGC
TTGAAGGTCATGGCCGGGCTGGACATTTCCGAGCGCCGCAAGCCGCAGGACGGCCGCATCAAGATGCGCGTCTCGAAAAC
CAAATCCATTGACTTCCGCGTCAACACTCTCCCGACCCTCTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCGGCCA
GCGCACAGATGGGTATCGATGCTCTAGGTTACGAAGACAACCAGAAGCAACTCTATCTGGAGGCGCTGAAGCAACCGCAA
GGCATGATCCTGGTAACCGGCCCCACCGGTTCGGGCAAGACAGTATCCCTCTATACCGGCCTGAATATCCTCAATACCCC
GGACATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAATGTGAATCCCAAGC
AGGGCATGGATTTCTCCACAGCCTTGCGCGCGTTTCTGCGTCAGGACCCAGATGTGATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATTGCGATCAAGGCCGCGCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCTGAACATGGGCGTGGCCTCGTTCAACCTGGCTACCTCGGTCAACCTGATCATTGCCCAGC
GTCTGGCACGCAAGTTGTGTTCGGCTTGCAAGAAGGAACACGAGGTTCCGCGCGAAGCCCTGCTCCACGAAGGCTTCCCG
GAAGACAGGATCGGTACCTTCAAGCTCTACTCGCCGGTGGGGTGTGAAAATTGCAACGGTGGGTACAAGGGCCGTGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAGGAAGGCTTCAATGATTTGCGTACCTCGGGCCTCCTGAAAGCTATGCAAGGCATTACGAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.938

99.647

0.587
  pilB Acinetobacter baylyi ADP1

58.511

99.471

0.582
  pilB Legionella pneumophila strain ERS1305867

54.336

99.647

0.541
  pilF Neisseria gonorrhoeae MS11

51.413

99.824

0.513
  pilB Vibrio cholerae strain A1552

50.617

100

0.506
  pilB Vibrio campbellii strain DS40M4

50.177

99.824

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.75

95.767

0.496
  pilF Thermus thermophilus HB27

38.957

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.925

93.651

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

39.347

91.887

0.362

Multiple sequence alignment