Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AB8Z76_RS16940 Genome accession   NZ_CP166098
Coordinates   3872505..3872945 (-) Length   146 a.a.
NCBI ID   WP_039568292.1    Uniprot ID   A0AB34QK76
Organism   Xanthomonas phaseoli pv. phaseoli strain NCPPB 3035     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3867505..3877945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8Z76_RS16915 (AB8Z76_16890) pilR 3867916..3869370 (+) 1455 WP_039568287.1 sigma-54 dependent transcriptional regulator Regulator
  AB8Z76_RS16920 (AB8Z76_16895) - 3869567..3869713 (-) 147 WP_080948799.1 serine/threonine protein kinase -
  AB8Z76_RS16925 (AB8Z76_16900) - 3869775..3869894 (-) 120 WP_039568289.1 hypothetical protein -
  AB8Z76_RS16930 (AB8Z76_16905) pilB 3870139..3871848 (-) 1710 WP_180959844.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AB8Z76_RS16935 (AB8Z76_16910) - 3871940..3872395 (-) 456 WP_039568290.1 pilin -
  AB8Z76_RS16940 (AB8Z76_16915) pilA 3872505..3872945 (-) 441 WP_039568292.1 pilin Machinery gene
  AB8Z76_RS16945 (AB8Z76_16920) pilC 3873300..3874556 (+) 1257 WP_039573972.1 type II secretion system F family protein Machinery gene
  AB8Z76_RS16950 (AB8Z76_16925) - 3874563..3875426 (+) 864 WP_039573976.1 A24 family peptidase -
  AB8Z76_RS16955 (AB8Z76_16930) coaE 3875437..3876048 (+) 612 WP_007962470.1 dephospho-CoA kinase -
  AB8Z76_RS16960 (AB8Z76_16935) - 3876230..3877300 (+) 1071 WP_157926032.1 RHS repeat domain-containing protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15218.48 Da        Isoelectric Point: 8.4936

>NTDB_id=1033403 AB8Z76_RS16940 WP_039568292.1 3872505..3872945(-) (pilA) [Xanthomonas phaseoli pv. phaseoli strain NCPPB 3035]
MKKQHGFTLIELMIVIAIIAILAAIALPQYQNYVAKSQVTAGLAELSPGKTQYETLLNEGKGSTLTTADAVGLQASSTTN
RCSTITVLAPDATTGAQTNALSCTLAGSPRIVGKKITWARDTNGVWTCSTDIATGDKAKFAPATCQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1033403 AB8Z76_RS16940 WP_039568292.1 3872505..3872945(-) (pilA) [Xanthomonas phaseoli pv. phaseoli strain NCPPB 3035]
ATGAAGAAGCAACACGGTTTCACTCTGATCGAACTCATGATCGTCATCGCGATCATTGCAATCTTGGCCGCCATTGCACT
GCCGCAGTACCAGAACTATGTCGCAAAGTCGCAGGTCACTGCTGGTCTGGCCGAGCTGAGCCCGGGCAAGACGCAGTACG
AAACTCTTTTGAACGAAGGCAAGGGCTCGACGCTGACGACTGCTGATGCAGTTGGTCTGCAGGCCTCGTCGACCACCAAT
CGCTGTTCGACGATTACGGTGTTGGCTCCGGATGCTACAACCGGTGCGCAGACTAACGCTTTGAGCTGCACGCTGGCCGG
TAGCCCGCGTATTGTTGGTAAGAAGATCACTTGGGCTCGTGATACCAACGGTGTTTGGACCTGCTCGACTGATATCGCGA
CTGGTGATAAGGCAAAGTTCGCTCCAGCTACCTGTCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.966

99.315

0.486
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.452
  pilA Vibrio cholerae strain A1552

41.781

100

0.418
  pilA Vibrio cholerae C6706

41.781

100

0.418
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.781

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

98.63

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.259

97.945

0.404
  comP Acinetobacter baylyi ADP1

36.709

100

0.397
  pilE Neisseria gonorrhoeae strain FA1090

34.783

100

0.384
  pilA Ralstonia pseudosolanacearum GMI1000

33.133

100

0.377
  pilE Neisseria gonorrhoeae MS11

34.161

100

0.377

Multiple sequence alignment