Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB4Y39_RS20590 Genome accession   NZ_CP162607
Coordinates   4459623..4460879 (-) Length   418 a.a.
NCBI ID   WP_052675472.1    Uniprot ID   A0AB39BAW6
Organism   Pseudomonas sp. Hg7Tf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4454623..4465879
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4Y39_RS20575 (AB4Y39_20575) - 4456285..4457886 (-) 1602 WP_101293751.1 SPOR domain-containing protein -
  AB4Y39_RS20580 (AB4Y39_20580) aroB 4457903..4459000 (-) 1098 WP_045181738.1 3-dehydroquinate synthase -
  AB4Y39_RS20585 (AB4Y39_20585) aroK 4459100..4459618 (-) 519 WP_028943928.1 shikimate kinase AroK -
  AB4Y39_RS20590 (AB4Y39_20590) pilQ 4459623..4460879 (-) 1257 WP_052675472.1 type IV pilus secretin PilQ Machinery gene
  AB4Y39_RS20595 (AB4Y39_20595) - 4460940..4461800 (-) 861 WP_368655848.1 pilus assembly protein PilP -
  AB4Y39_RS20600 (AB4Y39_20600) - 4461872..4462399 (-) 528 WP_274071345.1 PilN domain-containing protein -
  AB4Y39_RS20605 (AB4Y39_20605) pilM 4462387..4463325 (-) 939 WP_274071346.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45479.95 Da        Isoelectric Point: 4.7968

>NTDB_id=1027452 AB4Y39_RS20590 WP_052675472.1 4459623..4460879(-) (pilQ) [Pseudomonas sp. Hg7Tf]
MVFSLQAVSEVSVSSVSPGEPLSLNFQDVEVRSVLQVLADFSGVNLVASDAVQGRVTLRLQDVPWEQALDLVLRSKGLEK
RTEGNVLLVAPIGELSGHERQLLEGRQQVAGLAPTRRELLAIHYAQAKDIAAVFQSLMDNDPHDFAQGALSVDERTNTLI
ARQTPERLEVLRQLIARLDVPVRQVMIEARIVEANVDYERSLGVRWGAEVNLGGGLVLGGGDTFVDLGAERATSAIGLGL
VRDNLLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSGSGATSVTFREASLSLEVTPQITPDNRVIM
AVKVTKDEPDYLNALAQVPPIRKNEVNAKVRVSDGETIVIGGVYSTVQSKVVDKVPFFGDVPYVGRLFRRDVLQEKKSEL
LVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=1027452 AB4Y39_RS20590 WP_052675472.1 4459623..4460879(-) (pilQ) [Pseudomonas sp. Hg7Tf]
ATGGTGTTTTCGCTGCAGGCCGTCAGTGAAGTTTCAGTCAGTAGCGTTTCACCGGGCGAGCCGTTGTCATTGAACTTCCA
GGATGTCGAGGTTCGCTCGGTGTTACAGGTGCTGGCCGATTTTTCCGGGGTTAATCTGGTCGCCAGCGATGCGGTGCAGG
GCAGGGTGACCCTACGCCTGCAGGATGTGCCGTGGGAGCAGGCTCTGGACTTGGTGCTGCGCAGCAAGGGCTTGGAGAAA
CGCACCGAAGGCAATGTGTTGCTGGTGGCACCTATCGGCGAGCTCTCTGGGCATGAGCGGCAACTGCTGGAAGGGCGCCA
ACAGGTAGCGGGCTTGGCGCCCACACGACGTGAGTTGCTGGCGATCCACTATGCCCAGGCTAAGGATATAGCCGCCGTGT
TCCAGTCACTGATGGATAACGATCCCCACGACTTTGCTCAGGGTGCATTGAGTGTCGATGAGCGAACCAACACCCTGATC
GCCCGCCAGACACCGGAACGACTGGAGGTGCTGCGCCAGTTGATCGCGCGACTGGATGTGCCCGTACGGCAGGTGATGAT
CGAGGCGCGGATTGTCGAGGCTAACGTCGACTACGAGCGAAGCCTGGGCGTGCGCTGGGGGGCAGAGGTGAATCTAGGAG
GTGGTCTGGTGTTGGGCGGTGGCGACACCTTTGTCGACCTTGGTGCAGAACGGGCCACCTCGGCGATTGGGCTGGGCCTG
GTGCGCGATAACCTGTTGTTGGACCTGGAGCTCAGTGCCATGGAGAAATCCGGCAACGGTGAAATCATTTCCCAGCCCAA
GGTAGTGACTGCCGACAAGGAAACCGCGCGGATCCTCAAGGGCACCGAAGTGCCCTATCAGGAAACCAGTGGCAGCGGCG
CTACGTCGGTGACCTTCCGTGAAGCGTCGCTGTCGTTGGAGGTGACGCCGCAGATAACCCCGGACAACCGGGTGATCATG
GCCGTCAAGGTCACCAAGGATGAACCGGACTACCTCAACGCCCTGGCCCAGGTGCCGCCGATCAGGAAGAACGAGGTAAA
CGCCAAGGTTCGCGTCAGCGATGGCGAGACCATCGTGATTGGCGGCGTGTACTCAACGGTGCAAAGTAAAGTTGTCGACA
AGGTGCCATTTTTCGGCGATGTGCCGTATGTTGGGCGGCTGTTTCGTCGCGATGTTCTACAAGAGAAAAAATCCGAGCTG
CTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(253-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-184)

Secretin and TonB N terminus short domain (STN)

(45-90)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

61.521

100

0.639
  comQ Acinetobacter baylyi ADP1

42.022

100

0.447
  pilQ Acinetobacter baumannii strain A118

40.347

100

0.445
  pilQ Acinetobacter baumannii D1279779

40.347

100

0.445
  pilQ Legionella pneumophila strain ERS1305867

42.755

100

0.431
  pilQ Legionella pneumophila strain Lp02

42.755

100

0.431
  pilQ Vibrio campbellii strain DS40M4

41.492

100

0.426
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.827

99.522

0.416
  pilQ Vibrio cholerae strain A1552

41.827

99.522

0.416
  pilQ Neisseria meningitidis MC58

37.857

100

0.38
  pilQ Neisseria meningitidis M1080

37.619

100

0.378
  pilQ Neisseria gonorrhoeae strain FA1090

37.381

100

0.376

Multiple sequence alignment