Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB3X07_RS07335 Genome accession   NZ_CP162488
Coordinates   1738247..1739377 (+) Length   376 a.a.
NCBI ID   WP_184410693.1    Uniprot ID   -
Organism   Xanthomonas medicagonis strain DAR 35659     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1733247..1744377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3X07_RS07310 (AB3X07_07310) - 1733509..1733964 (-) 456 WP_369943770.1 DUF4426 domain-containing protein -
  AB3X07_RS07315 (AB3X07_07315) proC 1733979..1734821 (-) 843 WP_369943771.1 pyrroline-5-carboxylate reductase -
  AB3X07_RS07320 (AB3X07_07320) - 1734952..1735980 (-) 1029 WP_369944683.1 hypothetical protein -
  AB3X07_RS07325 (AB3X07_07325) - 1736299..1736949 (-) 651 WP_369944685.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3X07_RS07330 (AB3X07_07330) pilT 1737072..1738109 (+) 1038 WP_043095750.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3X07_RS07335 (AB3X07_07335) pilU 1738247..1739377 (+) 1131 WP_184410693.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB3X07_RS07340 (AB3X07_07340) - 1739537..1740799 (+) 1263 WP_369943772.1 WD40 repeat domain-containing protein -
  AB3X07_RS07345 (AB3X07_07345) - 1740796..1742295 (+) 1500 WP_369943773.1 sensor domain-containing diguanylate cyclase -
  AB3X07_RS07350 (AB3X07_07350) - 1742531..1742995 (+) 465 WP_369943774.1 MFS transporter permease -
  AB3X07_RS07355 (AB3X07_07355) - 1743306..1743980 (+) 675 WP_369944686.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41896.19 Da        Isoelectric Point: 6.8811

>NTDB_id=1026435 AB3X07_RS07335 WP_184410693.1 1738247..1739377(+) (pilU) [Xanthomonas medicagonis strain DAR 35659]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTSQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1026435 AB3X07_RS07335 WP_184410693.1 1738247..1739377(+) (pilU) [Xanthomonas medicagonis strain DAR 35659]
ATGAGCACCATCGACTTCACTTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACGCCGATCACGCAGACGCCGCTGACCTCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAATTTCGCCATCGGCGTGGCCGGG
GTCGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTCGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCATGCCATCTCCTTCGCCGAAACCGGCCACCTCGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGTTGCTGATGGACCTGTCGCTTAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGGCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGGCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCAAGACCCTGGCGCAGGGGCTGGACGGGGTGGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.571

93.085

0.396
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362

Multiple sequence alignment