Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC/cglC   Type   Machinery gene
Locus tag   ABXJ36_RS04520 Genome accession   NZ_CP159631
Coordinates   899353..899628 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain Z217-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 856355..899329 899353..899628 flank 24


Gene organization within MGE regions


Location: 856355..899628
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXJ36_RS04220 (ABXJ36_04220) - 856355..857362 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  ABXJ36_RS04225 (ABXJ36_04225) comGG 857491..857844 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  ABXJ36_RS04230 (ABXJ36_04230) comGF 857844..858278 (-) 435 WP_033626933.1 competence type IV pilus minor pilin ComGF -
  ABXJ36_RS04235 (ABXJ36_04235) - 858268..858669 (-) 402 WP_002410542.1 type II secretion system protein -
  ABXJ36_RS04240 (ABXJ36_04240) hemH 859396..860337 (-) 942 WP_002364185.1 ferrochelatase -
  ABXJ36_RS04250 (ABXJ36_04250) - 861077..861325 (-) 249 WP_002383136.1 hypothetical protein -
  ABXJ36_RS04255 (ABXJ36_04255) - 861397..861597 (-) 201 WP_002357053.1 cold-shock protein -
  ABXJ36_RS04260 (ABXJ36_04260) - 862434..863675 (-) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  ABXJ36_RS04265 (ABXJ36_04265) - 863676..863909 (-) 234 WP_002384371.1 phage holin -
  ABXJ36_RS04270 (ABXJ36_04270) - 863902..864123 (-) 222 WP_002364191.1 hypothetical protein -
  ABXJ36_RS04275 (ABXJ36_04275) - 864158..864313 (-) 156 WP_002364192.1 XkdX family protein -
  ABXJ36_RS04280 (ABXJ36_04280) - 864315..864635 (-) 321 WP_002389262.1 hypothetical protein -
  ABXJ36_RS04285 (ABXJ36_04285) - 864649..865140 (-) 492 WP_002364194.1 hypothetical protein -
  ABXJ36_RS04290 (ABXJ36_04290) - 865140..865427 (-) 288 WP_002357046.1 collagen-like protein -
  ABXJ36_RS04295 (ABXJ36_04295) - 865424..866020 (-) 597 WP_002357045.1 hypothetical protein -
  ABXJ36_RS04300 (ABXJ36_04300) - 866013..866894 (-) 882 WP_002387290.1 phage baseplate upper protein -
  ABXJ36_RS04305 (ABXJ36_04305) - 866913..869720 (-) 2808 WP_002384367.1 phage tail spike protein -
  ABXJ36_RS04310 (ABXJ36_04310) - 869702..870436 (-) 735 WP_002403868.1 tail protein -
  ABXJ36_RS04315 (ABXJ36_04315) - 870426..873323 (-) 2898 WP_354017711.1 tape measure protein -
  ABXJ36_RS04320 (ABXJ36_04320) - 873571..873921 (-) 351 WP_002357039.1 hypothetical protein -
  ABXJ36_RS04325 (ABXJ36_04325) - 873974..874822 (-) 849 WP_002387287.1 major tail protein -
  ABXJ36_RS04330 (ABXJ36_04330) - 874823..875197 (-) 375 WP_002387286.1 DUF6838 family protein -
  ABXJ36_RS04335 (ABXJ36_04335) - 875200..875598 (-) 399 WP_002357036.1 HK97 gp10 family phage protein -
  ABXJ36_RS04340 (ABXJ36_04340) - 875591..875965 (-) 375 WP_002387285.1 hypothetical protein -
  ABXJ36_RS04345 (ABXJ36_04345) - 875962..876306 (-) 345 WP_002387282.1 hypothetical protein -
  ABXJ36_RS04350 (ABXJ36_04350) - 876320..876502 (-) 183 WP_002387281.1 hypothetical protein -
  ABXJ36_RS04355 (ABXJ36_04355) - 876531..877418 (-) 888 WP_354017701.1 DUF5309 domain-containing protein -
  ABXJ36_RS04360 (ABXJ36_04360) - 877432..878055 (-) 624 WP_010706493.1 DUF4355 domain-containing protein -
  ABXJ36_RS04365 (ABXJ36_04365) - 878274..878594 (-) 321 WP_002380436.1 hypothetical protein -
  ABXJ36_RS04370 (ABXJ36_04370) - 878597..879643 (-) 1047 WP_311812566.1 phage head morphogenesis protein -
  ABXJ36_RS04375 (ABXJ36_04375) - 879618..881090 (-) 1473 WP_010717875.1 phage portal protein -
  ABXJ36_RS04380 (ABXJ36_04380) terL 881102..882490 (-) 1389 WP_029657252.1 phage terminase large subunit -
  ABXJ36_RS04385 (ABXJ36_04385) - 882483..883202 (-) 720 WP_010716856.1 terminase small subunit -
  ABXJ36_RS04390 (ABXJ36_04390) - 883217..883447 (-) 231 WP_010711272.1 hypothetical protein -
  ABXJ36_RS04395 (ABXJ36_04395) - 883569..883760 (-) 192 WP_354017700.1 hypothetical protein -
  ABXJ36_RS04400 (ABXJ36_04400) - 883798..885132 (-) 1335 WP_033599682.1 DNA methyltransferase -
  ABXJ36_RS04405 (ABXJ36_04405) - 885135..885368 (-) 234 WP_033599680.1 hypothetical protein -
  ABXJ36_RS04410 (ABXJ36_04410) - 885989..886669 (-) 681 WP_010820923.1 hypothetical protein -
  ABXJ36_RS04415 (ABXJ36_04415) - 886683..887621 (-) 939 WP_010820924.1 hypothetical protein -
  ABXJ36_RS04425 (ABXJ36_04425) - 888331..888747 (-) 417 WP_002357018.1 ArpU family phage packaging/lysis transcriptional regulator -
  ABXJ36_RS04430 (ABXJ36_04430) - 889655..890063 (-) 409 Protein_860 RusA family crossover junction endodeoxyribonuclease -
  ABXJ36_RS04435 (ABXJ36_04435) - 890060..890929 (-) 870 WP_354017699.1 helix-turn-helix domain-containing protein -
  ABXJ36_RS04440 (ABXJ36_04440) - 890929..891129 (-) 201 WP_354017698.1 hypothetical protein -
  ABXJ36_RS04445 (ABXJ36_04445) - 891134..891775 (-) 642 WP_002364344.1 putative HNHc nuclease -
  ABXJ36_RS04450 (ABXJ36_04450) - 891780..892514 (-) 735 WP_002383799.1 ERF family protein -
  ABXJ36_RS04455 (ABXJ36_04455) - 892507..892824 (-) 318 WP_002383798.1 hypothetical protein -
  ABXJ36_RS04460 (ABXJ36_04460) - 893020..893358 (-) 339 WP_354017697.1 hypothetical protein -
  ABXJ36_RS04465 (ABXJ36_04465) - 893395..893604 (-) 210 WP_010828872.1 hypothetical protein -
  ABXJ36_RS04470 (ABXJ36_04470) - 893659..893847 (+) 189 WP_002357001.1 YegP family protein -
  ABXJ36_RS04475 (ABXJ36_04475) - 893873..894598 (-) 726 WP_338349015.1 ORF6C domain-containing protein -
  ABXJ36_RS04480 (ABXJ36_04480) - 894637..894954 (-) 318 WP_002356999.1 hypothetical protein -
  ABXJ36_RS04485 (ABXJ36_04485) - 894960..895151 (-) 192 WP_002356998.1 hypothetical protein -
  ABXJ36_RS04490 (ABXJ36_04490) - 895443..895784 (+) 342 WP_002396615.1 helix-turn-helix transcriptional regulator -
  ABXJ36_RS04495 (ABXJ36_04495) - 895789..896436 (+) 648 WP_311812563.1 ImmA/IrrE family metallo-endopeptidase -
  ABXJ36_RS04500 (ABXJ36_04500) - 896536..897318 (+) 783 WP_354017710.1 LysM domain-containing protein -
  ABXJ36_RS04505 (ABXJ36_04505) - 897333..897662 (+) 330 WP_311812561.1 hypothetical protein -
  ABXJ36_RS04510 (ABXJ36_04510) - 897737..898885 (+) 1149 WP_311812560.1 site-specific integrase -
  ABXJ36_RS04515 (ABXJ36_04515) comGD 898922..899356 (-) 435 Protein_877 competence type IV pilus minor pilin ComGD -
  ABXJ36_RS04520 (ABXJ36_04520) comGC/cglC 899353..899628 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence


Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=1015054 ABXJ36_RS04520 WP_002356991.1 899353..899628(-) (comGC/cglC) [Enterococcus faecalis strain Z217-1]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=1015054 ABXJ36_RS04520 WP_002356991.1 899353..899628(-) (comGC/cglC) [Enterococcus faecalis strain Z217-1]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582

  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582

  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582

  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582

  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571

  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571

  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549

  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538

  comYC Streptococcus suis isolate S10

52.326

94.505

0.495

  comYC Streptococcus mutans UA159

57.692

85.714

0.495

  comYC Streptococcus mutans UA140

57.692

85.714

0.495

  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462

  comGC Staphylococcus aureus MW2

46.835

86.813

0.407

  comGC Staphylococcus aureus N315

46.835

86.813

0.407

  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396


Multiple sequence alignment