Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   ABXJ33_RS04745 Genome accession   NZ_CP159629
Coordinates   1003646..1003921 (+) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain Z217-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1003945..1056309 1003646..1003921 flank 24


Gene organization within MGE regions


Location: 1003646..1056309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXJ33_RS04745 (ABXJ33_04745) comGC/cglC 1003646..1003921 (+) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  ABXJ33_RS04750 (ABXJ33_04750) comGD 1003918..1004352 (+) 435 Protein_929 competence type IV pilus minor pilin ComGD -
  ABXJ33_RS04755 (ABXJ33_04755) - 1004389..1005537 (-) 1149 WP_311812560.1 site-specific integrase -
  ABXJ33_RS04760 (ABXJ33_04760) - 1005612..1005941 (-) 330 WP_311812561.1 hypothetical protein -
  ABXJ33_RS04765 (ABXJ33_04765) - 1005956..1006738 (-) 783 WP_354017710.1 LysM domain-containing protein -
  ABXJ33_RS04770 (ABXJ33_04770) - 1006838..1007485 (-) 648 WP_311812563.1 ImmA/IrrE family metallo-endopeptidase -
  ABXJ33_RS04775 (ABXJ33_04775) - 1007490..1007831 (-) 342 WP_002396615.1 helix-turn-helix transcriptional regulator -
  ABXJ33_RS04780 (ABXJ33_04780) - 1008123..1008314 (+) 192 WP_002356998.1 hypothetical protein -
  ABXJ33_RS04785 (ABXJ33_04785) - 1008320..1008637 (+) 318 WP_002356999.1 hypothetical protein -
  ABXJ33_RS04790 (ABXJ33_04790) - 1008676..1009401 (+) 726 WP_338349015.1 ORF6C domain-containing protein -
  ABXJ33_RS04795 (ABXJ33_04795) - 1009427..1009615 (-) 189 WP_002357001.1 YegP family protein -
  ABXJ33_RS04800 (ABXJ33_04800) - 1009670..1009879 (+) 210 WP_010828872.1 hypothetical protein -
  ABXJ33_RS04805 (ABXJ33_04805) - 1009916..1010254 (+) 339 WP_354017697.1 hypothetical protein -
  ABXJ33_RS04810 (ABXJ33_04810) - 1010450..1010767 (+) 318 WP_002383798.1 hypothetical protein -
  ABXJ33_RS04815 (ABXJ33_04815) - 1010760..1011494 (+) 735 WP_002383799.1 ERF family protein -
  ABXJ33_RS04820 (ABXJ33_04820) - 1011499..1012140 (+) 642 WP_002364344.1 putative HNHc nuclease -
  ABXJ33_RS04825 (ABXJ33_04825) - 1012145..1012345 (+) 201 WP_354017698.1 hypothetical protein -
  ABXJ33_RS04830 (ABXJ33_04830) - 1012345..1013214 (+) 870 WP_354017699.1 helix-turn-helix domain-containing protein -
  ABXJ33_RS04835 (ABXJ33_04835) - 1013211..1013619 (+) 409 Protein_946 RusA family crossover junction endodeoxyribonuclease -
  ABXJ33_RS04840 (ABXJ33_04840) - 1013899..1014159 (+) 261 WP_002383807.1 hypothetical protein -
  ABXJ33_RS04845 (ABXJ33_04845) - 1014527..1014943 (+) 417 WP_002357018.1 ArpU family phage packaging/lysis transcriptional regulator -
  ABXJ33_RS04855 (ABXJ33_04855) - 1015653..1016591 (+) 939 WP_010820924.1 hypothetical protein -
  ABXJ33_RS04860 (ABXJ33_04860) - 1016605..1017285 (+) 681 WP_010820923.1 hypothetical protein -
  ABXJ33_RS04865 (ABXJ33_04865) - 1017906..1018139 (+) 234 WP_033599680.1 hypothetical protein -
  ABXJ33_RS04870 (ABXJ33_04870) - 1018142..1019476 (+) 1335 WP_033599682.1 DNA methyltransferase -
  ABXJ33_RS04875 (ABXJ33_04875) - 1019514..1019705 (+) 192 WP_354017700.1 hypothetical protein -
  ABXJ33_RS04880 (ABXJ33_04880) - 1019827..1020057 (+) 231 WP_010711272.1 hypothetical protein -
  ABXJ33_RS04885 (ABXJ33_04885) - 1020072..1020791 (+) 720 WP_010716856.1 terminase small subunit -
  ABXJ33_RS04890 (ABXJ33_04890) terL 1020784..1022172 (+) 1389 WP_029657252.1 phage terminase large subunit -
  ABXJ33_RS04895 (ABXJ33_04895) - 1022184..1023656 (+) 1473 WP_010717875.1 phage portal protein -
  ABXJ33_RS04900 (ABXJ33_04900) - 1023631..1024677 (+) 1047 WP_311812566.1 phage head morphogenesis protein -
  ABXJ33_RS04905 (ABXJ33_04905) - 1024680..1025000 (+) 321 WP_002380436.1 hypothetical protein -
  ABXJ33_RS04910 (ABXJ33_04910) - 1025219..1025842 (+) 624 WP_010706493.1 DUF4355 domain-containing protein -
  ABXJ33_RS04915 (ABXJ33_04915) - 1025856..1026743 (+) 888 WP_354017701.1 DUF5309 domain-containing protein -
  ABXJ33_RS04920 (ABXJ33_04920) - 1026772..1026954 (+) 183 WP_002387281.1 hypothetical protein -
  ABXJ33_RS04925 (ABXJ33_04925) - 1026968..1027312 (+) 345 WP_002387282.1 hypothetical protein -
  ABXJ33_RS04930 (ABXJ33_04930) - 1027309..1027683 (+) 375 WP_002387285.1 hypothetical protein -
  ABXJ33_RS04935 (ABXJ33_04935) - 1027676..1028074 (+) 399 WP_002357036.1 HK97 gp10 family phage protein -
  ABXJ33_RS04940 (ABXJ33_04940) - 1028077..1028451 (+) 375 WP_002387286.1 DUF6838 family protein -
  ABXJ33_RS04945 (ABXJ33_04945) - 1028452..1029300 (+) 849 WP_002387287.1 major tail protein -
  ABXJ33_RS04950 (ABXJ33_04950) - 1029353..1029703 (+) 351 WP_002357039.1 hypothetical protein -
  ABXJ33_RS04955 (ABXJ33_04955) - 1029951..1032848 (+) 2898 WP_354017711.1 tape measure protein -
  ABXJ33_RS04960 (ABXJ33_04960) - 1032838..1033572 (+) 735 WP_002403868.1 tail protein -
  ABXJ33_RS04965 (ABXJ33_04965) - 1033554..1036361 (+) 2808 WP_002384367.1 phage tail spike protein -
  ABXJ33_RS04970 (ABXJ33_04970) - 1036380..1037261 (+) 882 WP_002387290.1 phage baseplate upper protein -
  ABXJ33_RS04975 (ABXJ33_04975) - 1037254..1037850 (+) 597 WP_002357045.1 hypothetical protein -
  ABXJ33_RS04980 (ABXJ33_04980) - 1037847..1038134 (+) 288 WP_002357046.1 collagen-like protein -
  ABXJ33_RS04985 (ABXJ33_04985) - 1038134..1038625 (+) 492 WP_002364194.1 hypothetical protein -
  ABXJ33_RS04990 (ABXJ33_04990) - 1038639..1038959 (+) 321 WP_002389262.1 hypothetical protein -
  ABXJ33_RS04995 (ABXJ33_04995) - 1038961..1039116 (+) 156 WP_002364192.1 XkdX family protein -
  ABXJ33_RS05000 (ABXJ33_05000) - 1039151..1039372 (+) 222 WP_002364191.1 hypothetical protein -
  ABXJ33_RS05005 (ABXJ33_05005) - 1039365..1039598 (+) 234 WP_002384371.1 phage holin -
  ABXJ33_RS05010 (ABXJ33_05010) - 1039599..1040840 (+) 1242 WP_002370962.1 LysM peptidoglycan-binding domain-containing protein -
  ABXJ33_RS05015 (ABXJ33_05015) - 1041677..1041877 (+) 201 WP_002357053.1 cold-shock protein -
  ABXJ33_RS05020 (ABXJ33_05020) - 1041949..1042197 (+) 249 WP_002383136.1 hypothetical protein -
  ABXJ33_RS05030 (ABXJ33_05030) hemH 1042937..1043878 (+) 942 WP_002364185.1 ferrochelatase -
  ABXJ33_RS05035 (ABXJ33_05035) - 1044175..1044300 (-) 126 WP_002364184.1 hypothetical protein -
  ABXJ33_RS05040 (ABXJ33_05040) - 1044605..1045006 (+) 402 WP_002410542.1 type II secretion system protein -
  ABXJ33_RS05045 (ABXJ33_05045) comGF 1044996..1045430 (+) 435 WP_033626933.1 competence type IV pilus minor pilin ComGF -
  ABXJ33_RS05050 (ABXJ33_05050) comGG 1045430..1045783 (+) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  ABXJ33_RS05055 (ABXJ33_05055) - 1045912..1046919 (+) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  ABXJ33_RS05060 (ABXJ33_05060) - 1046944..1048131 (+) 1188 WP_033626932.1 acetate kinase -
  ABXJ33_RS05065 (ABXJ33_05065) - 1048243..1048716 (-) 474 WP_002357065.1 universal stress protein -
  ABXJ33_RS05070 (ABXJ33_05070) - 1049034..1049312 (-) 279 WP_354017702.1 hypothetical protein -
  ABXJ33_RS05080 (ABXJ33_05080) - 1049639..1050916 (-) 1278 WP_002360030.1 replication-associated recombination protein A -
  ABXJ33_RS05085 (ABXJ33_05085) - 1051045..1051722 (+) 678 WP_002364174.1 DNA-3-methyladenine glycosylase -
  ABXJ33_RS05090 (ABXJ33_05090) - 1051679..1052164 (+) 486 WP_002388170.1 DUF3013 family protein -
  ABXJ33_RS05095 (ABXJ33_05095) prmA 1052180..1053127 (+) 948 WP_002357075.1 50S ribosomal protein L11 methyltransferase -
  ABXJ33_RS05100 (ABXJ33_05100) - 1053129..1053881 (+) 753 WP_002357078.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ABXJ33_RS05105 (ABXJ33_05105) - 1054096..1056309 (+) 2214 WP_002357079.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=1015001 ABXJ33_RS04745 WP_002356991.1 1003646..1003921(+) (comGC/cglC) [Enterococcus faecalis strain Z217-3]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=1015001 ABXJ33_RS04745 WP_002356991.1 1003646..1003921(+) (comGC/cglC) [Enterococcus faecalis strain Z217-3]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment