Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABVR74_RS00765 Genome accession   NZ_CP159484
Coordinates   162534..163472 (+) Length   312 a.a.
NCBI ID   WP_353891726.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain CBA3648     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 157534..168472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVR74_RS00765 (ABVR74_00765) comGA 162534..163472 (+) 939 WP_353891726.1 competence type IV pilus ATPase ComGA Machinery gene
  ABVR74_RS00770 (ABVR74_00770) comGB 163420..164439 (+) 1020 WP_058223571.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABVR74_RS00775 (ABVR74_00775) comGC 164566..164835 (+) 270 WP_023188581.1 competence type IV pilus major pilin ComGC Machinery gene
  ABVR74_RS00780 (ABVR74_00780) comGD 164810..165226 (+) 417 WP_025017137.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABVR74_RS00785 (ABVR74_00785) comGE 165198..165494 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABVR74_RS00790 (ABVR74_00790) comGF 165457..165903 (+) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABVR74_RS00795 (ABVR74_00795) comGG 165942..166226 (+) 285 WP_025017139.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABVR74_RS00800 (ABVR74_00800) - 166308..166745 (+) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  ABVR74_RS00805 (ABVR74_00805) - 166742..167584 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35755.97 Da        Isoelectric Point: 6.1811

>NTDB_id=1013800 ABVR74_RS00765 WP_353891726.1 162534..163472(+) (comGA) [Lactococcus lactis subsp. lactis strain CBA3648]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
IEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIYSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1013800 ABVR74_RS00765 WP_353891726.1 162534..163472(+) (comGA) [Lactococcus lactis subsp. lactis strain CBA3648]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
ATAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGAAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTTATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.603

100

0.766
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.984

99.679

0.548
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.757

100

0.519

Multiple sequence alignment