Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ABVR74_RS00770 Genome accession   NZ_CP159484
Coordinates   163420..164439 (+) Length   339 a.a.
NCBI ID   WP_058223571.1    Uniprot ID   A0AAC9R6N8
Organism   Lactococcus lactis subsp. lactis strain CBA3648     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 158420..169439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVR74_RS00765 (ABVR74_00765) comGA 162534..163472 (+) 939 WP_353891726.1 competence type IV pilus ATPase ComGA Machinery gene
  ABVR74_RS00770 (ABVR74_00770) comGB 163420..164439 (+) 1020 WP_058223571.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABVR74_RS00775 (ABVR74_00775) comGC 164566..164835 (+) 270 WP_023188581.1 competence type IV pilus major pilin ComGC Machinery gene
  ABVR74_RS00780 (ABVR74_00780) comGD 164810..165226 (+) 417 WP_025017137.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABVR74_RS00785 (ABVR74_00785) comGE 165198..165494 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABVR74_RS00790 (ABVR74_00790) comGF 165457..165903 (+) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABVR74_RS00795 (ABVR74_00795) comGG 165942..166226 (+) 285 WP_025017139.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABVR74_RS00800 (ABVR74_00800) - 166308..166745 (+) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  ABVR74_RS00805 (ABVR74_00805) - 166742..167584 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  ABVR74_RS00810 (ABVR74_00810) - 167761..168498 (+) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  ABVR74_RS00815 (ABVR74_00815) - 168491..169300 (+) 810 WP_014570791.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38300.01 Da        Isoelectric Point: 10.1935

>NTDB_id=1013801 ABVR74_RS00770 WP_058223571.1 163420..164439(+) (comGB) [Lactococcus lactis subsp. lactis strain CBA3648]
MDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLASGQALSELLESLSFSKNV
VTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQLNSGSNFATLLINHLPLV
FFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVELRQIINLMKKQKSRIFSE
VGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEHFFTKIDRLMQLIQPLVFIFVALMI
ILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=1013801 ABVR74_RS00770 WP_058223571.1 163420..164439(+) (comGB) [Lactococcus lactis subsp. lactis strain CBA3648]
ATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGG
AAATCTTTTGAATAGTGGATTTAATTTAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTA
CATTAAAAATGAAAGAAGGTCTCGCTTCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTG
GTGACACAACTTGCTTTGGTAGAGGTGCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACA
GCTTAAAGTTAAAAATAAATTAGTGGAAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGG
GCTTAAAAAATTATTTACTACCACAACTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTA
TTTTTTTCGTTTGGAGCTTTTATATTTTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAA
TTTTACATTTCTTGTTAAATTACCTCTGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGG
GAAATCTAATTGCTCAAGGTGTTGAATTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAA
GTTGGCAAGAATCTTGATTTAGAATTGAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACC
CGAGCTCTCATTAATGATTGAATATGGAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTT
GGGAACATTTTTTTACAAAGATAGATCGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATT
ATTTTGCTATATGCAGCCATGTTACTCCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.728

99.705

0.835
  comYB Streptococcus gordonii str. Challis substr. CH1

53.892

98.525

0.531
  comGB/cglB Streptococcus mitis SK321

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae D39

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae R6

51.045

98.82

0.504
  comYB Streptococcus mutans UA140

53.968

92.92

0.501
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

98.82

0.499
  comYB Streptococcus mutans UA159

53.651

92.92

0.499

Multiple sequence alignment