Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JMW08_RS18495 Genome accession   NZ_LR890288
Coordinates   3879583..3880023 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain MSB1_1A isolate MSB1_1A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3874583..3885023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JMW08_RS18475 aroP 3875581..3876954 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  JMW08_RS18480 ampE 3876997..3877851 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  JMW08_RS18485 ampD 3877848..3878399 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  JMW08_RS18490 nadC 3878487..3879380 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  JMW08_RS18495 pilA 3879583..3880023 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  JMW08_RS18500 pilB 3880033..3881418 (+) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  JMW08_RS18505 hofC 3881408..3882610 (+) 1203 WP_000157266.1 protein transport protein HofC -
  JMW08_RS18510 guaC 3882645..3883688 (-) 1044 WP_001217338.1 GMP reductase -
  JMW08_RS18515 - 3883844..3883888 (-) 45 WP_120795372.1 protein YacM -
  JMW08_RS18520 coaE 3883913..3884533 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=1013801 JMW08_RS18495 WP_000360904.1 3879583..3880023(+) (pilA) [Escherichia coli strain MSB1_1A isolate MSB1_1A]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=1013801 JMW08_RS18495 WP_000360904.1 3879583..3880023(+) (pilA) [Escherichia coli strain MSB1_1A isolate MSB1_1A]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment