Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   P303_RS00190 Genome accession   NZ_CP006740
Coordinates   5930..6364 (-) Length   144 a.a.
NCBI ID   WP_038228615.1    Uniprot ID   -
Organism   Xylella fastidiosa MUL0034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 930..11364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P303_RS00180 (P303_00185) - 1851..3263 (+) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -
  P303_RS00185 (P303_00190) pilB 3357..5090 (-) 1734 WP_012338105.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P303_RS00190 (P303_00195) pilA 5930..6364 (-) 435 WP_038228615.1 pilin Machinery gene
  P303_RS12135 (P303_00200) - 7197..7424 (+) 228 WP_012338102.1 hypothetical protein -
  P303_RS00195 (P303_00205) pilA 7669..8115 (-) 447 WP_040123054.1 pilin Machinery gene
  P303_RS00205 (P303_00210) pilC 8556..9758 (+) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  P303_RS00210 (P303_00215) pilD 9765..10628 (+) 864 WP_004084594.1 A24 family peptidase Machinery gene
  P303_RS00215 (P303_00220) coaE 10640..11263 (+) 624 WP_040123056.1 dephospho-CoA kinase -

Sequence


Protein


Download         Length: 144 a.a.        Molecular weight: 14996.38 Da        Isoelectric Point: 8.5024

>NTDB_id=100975 P303_RS00190 WP_038228615.1 5930..6364(-) (pilA) [Xylella fastidiosa MUL0034]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVTAGLAEITPSKAQAEIRIAEARPTTVSTDIGLRTPTSRCAV
AVDIKTDAESTITCTLIGNTQVNGQTIKLSRAVDNAAGTGGLWTCTTTVAEALAPGGCTSTKKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=100975 P303_RS00190 WP_038228615.1 5930..6364(-) (pilA) [Xylella fastidiosa MUL0034]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCCGCGATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCACAGCGGGACTGGCCGAAATTACACCCAGCAAAGCACAAGCCG
AAATCCGTATTGCCGAGGCAAGACCAACAACTGTTTCGACCGATATCGGACTGCGCACCCCAACGTCTCGTTGCGCCGTT
GCTGTCGACATCAAGACCGATGCTGAAAGCACAATTACATGTACGCTTATTGGTAATACCCAAGTCAACGGTCAAACCAT
TAAGTTGAGTCGCGCCGTCGATAACGCCGCAGGCACAGGGGGTCTGTGGACCTGTACCACAACCGTTGCCGAAGCGTTGG
CACCAGGAGGCTGCACTTCGACCAAGAAGAGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

46.897

100

0.472

  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.887

98.611

0.472

  pilA/pilAII Pseudomonas stutzeri DSM 10701

47.143

97.222

0.458

  pilA Pseudomonas aeruginosa PAK

41.026

100

0.444

  pilA Vibrio cholerae C6706

38.854

100

0.424

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.854

100

0.424

  pilA Vibrio cholerae strain A1552

38.854

100

0.424

  pilA Ralstonia pseudosolanacearum GMI1000

37.179

100

0.403

  pilA Vibrio parahaemolyticus RIMD 2210633

39.286

97.222

0.382

  comP Acinetobacter baylyi ADP1

35.333

100

0.368


Multiple sequence alignment