Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNF92_RS08245 Genome accession   NZ_CP157802
Coordinates   1782739..1783860 (-) Length   373 a.a.
NCBI ID   WP_069184769.1    Uniprot ID   A0A1E3C6D6
Organism   Marinobacter sp. MMG032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1777739..1788860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNF92_RS08230 (ABNF92_08230) polA 1777775..1780507 (+) 2733 WP_349343877.1 DNA polymerase I -
  ABNF92_RS08235 (ABNF92_08235) - 1780740..1781114 (+) 375 WP_069184770.1 hypothetical protein -
  ABNF92_RS08240 (ABNF92_08240) - 1781151..1782617 (-) 1467 WP_349343878.1 outer membrane protein transport protein -
  ABNF92_RS08245 (ABNF92_08245) pilU 1782739..1783860 (-) 1122 WP_069184769.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNF92_RS08250 (ABNF92_08250) pilT 1784077..1785111 (-) 1035 WP_058091364.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNF92_RS08255 (ABNF92_08255) - 1785252..1785956 (+) 705 WP_153634884.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNF92_RS08260 (ABNF92_08260) proC 1786018..1786851 (+) 834 WP_069184767.1 pyrroline-5-carboxylate reductase -
  ABNF92_RS08265 (ABNF92_08265) - 1786874..1787458 (+) 585 WP_064228405.1 YggT family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41484.65 Da        Isoelectric Point: 6.5039

>NTDB_id=1008367 ABNF92_RS08245 WP_069184769.1 1782739..1783860(-) (pilU) [Marinobacter sp. MMG032]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLSLPEVIKELAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRKAVIEVLINTPLVADLIRKGEVHRLKELMSKSNESGMQTFDQA
LYQLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDKLSIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1008367 ABNF92_RS08245 WP_069184769.1 1782739..1783860(-) (pilU) [Marinobacter sp. MMG032]
ATGGAATTCGAAAAACTGTTACGGCTGATGGTGGAAAAGGGCGGCTCGGACCTGTTTATTACGGCGGGCGTTCCCCCAAG
CATGAAGGTGAACGGGAAAGTGCTCCCGGTCACCAAGAACGCGCTGACGCCGGAGCAGACGAGGGAGTTTGTCTACGGCG
CCATGAACGACAAGCAGCGCGCCGAGTTTGAGGAAACCCACGAGTGCAACTTTGCCATCAGTGCCCGGGGCATTGGCCGG
TTCCGGGTGTCTGCGTTCTTCCAGCGCAACCTCTGTGGCATGGTGCTGCGTCGGATCGAGGTGAAGATTCCGCAGATTGA
CGACCTGTCGCTGCCGGAGGTTATCAAGGAACTCGCCATGACCAAGCGTGGCCTGATCATGTTCGTGGGCGCCACCGGTA
CCGGTAAGTCCACGTCCCTGGCGGCCATGCTGGGGCACCGGAATCGCAACAGCCGGGGCCACATCATTTCCATCGAAGAC
CCGATCGAATTCGTGCACCAGCACCAGGGCTGTATCGTCACCCAGCGTGAGGTGGGTATCGATACCGAGAGCTTCGAGGT
GGCCCTGAAGAACACGCTGCGTCAGGCGCCCGACGTTATCCTGATCGGCGAGGTGCGGACCCGGCAGACCATGGAGTATT
CCGTGCAGTTCGCCGAGACCGGTCACCTGTGTCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCCAGTTCTTCCCTCCGGAACAGCACAACCAGATCTGGATGGACCTGTCCCTGAACCTCAAGGCCATTGTGGCCCAGCA
ACTGGTGCCAACACCGGATGGCAAGGGACGTAAAGCGGTTATCGAAGTGCTGATCAATACCCCGCTGGTGGCGGATCTGA
TTCGGAAGGGCGAGGTGCATCGTCTGAAGGAGCTGATGTCGAAATCCAACGAGTCTGGCATGCAGACCTTTGACCAGGCC
CTGTATCAGTTGTACGCAGAGGGCTCCATTACCTACGAAGATGCCCTGGCCCATGCCGACTCTGCCAACGACCTGCGCCT
GATGATCAAACTGGGTGCCGACGCTCAGGGGGCGGACAAGCTGTCTTCCTCTGTGGACAAGCTCTCAATACAGGACGACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3C6D6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.515

99.464

0.791
  pilU Acinetobacter baylyi ADP1

64.607

95.442

0.617
  pilU Vibrio cholerae strain A1552

57.568

99.196

0.571
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

90.885

0.386
  pilT Pseudomonas aeruginosa PAK

40.896

89.812

0.367
  pilT Acinetobacter baumannii strain A118

40.597

89.812

0.365
  pilT Acinetobacter baumannii D1279779

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.597

89.812

0.365
  pilT Acinetobacter nosocomialis M2

40.299

89.812

0.362
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362

Multiple sequence alignment