Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   QUD80_RS08540 Genome accession   NZ_AP027302
Coordinates   1814341..1814616 (+) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain N4E     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1814640..1866327 1814341..1814616 flank 24


Gene organization within MGE regions


Location: 1814341..1866327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD80_RS08540 (N4E_16670) comGC/cglC 1814341..1814616 (+) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  QUD80_RS08545 (N4E_16680) comGD 1814613..1815047 (+) 435 Protein_1663 competence type IV pilus minor pilin ComGD -
  QUD80_RS08550 (N4E_16690) - 1815084..1816232 (-) 1149 WP_002387264.1 site-specific integrase -
  QUD80_RS08555 (N4E_16700) - 1816338..1816967 (-) 630 WP_002410530.1 SHOCT domain-containing protein -
  QUD80_RS08560 (N4E_16710) - 1817063..1817713 (-) 651 WP_002387266.1 ImmA/IrrE family metallo-endopeptidase -
  QUD80_RS08565 (N4E_16720) - 1817718..1818059 (-) 342 WP_002387267.1 helix-turn-helix transcriptional regulator -
  QUD80_RS08570 (N4E_16730) - 1818355..1818546 (+) 192 WP_002383936.1 hypothetical protein -
  QUD80_RS08575 (N4E_16740) - 1818558..1818869 (+) 312 WP_002403885.1 hypothetical protein -
  QUD80_RS08580 (N4E_16750) - 1818892..1819614 (+) 723 WP_002410531.1 ORF6C domain-containing protein -
  QUD80_RS08585 (N4E_16760) - 1819640..1819828 (-) 189 WP_002357001.1 YegP family protein -
  QUD80_RS08590 (N4E_16770) - 1819883..1820092 (+) 210 WP_002378465.1 hypothetical protein -
  QUD80_RS08595 (N4E_16780) - 1820129..1820467 (+) 339 WP_002402484.1 hypothetical protein -
  QUD80_RS08600 (N4E_16790) - 1820663..1820980 (+) 318 WP_002402485.1 hypothetical protein -
  QUD80_RS08605 (N4E_16800) - 1820973..1821707 (+) 735 WP_002402486.1 ERF family protein -
  QUD80_RS08610 (N4E_16810) - 1821712..1822353 (+) 642 WP_002402487.1 putative HNHc nuclease -
  QUD80_RS08615 (N4E_16820) - 1822358..1822558 (+) 201 WP_010816620.1 hypothetical protein -
  QUD80_RS08620 (N4E_16830) - 1822558..1823430 (+) 873 WP_010715319.1 helix-turn-helix domain-containing protein -
  QUD80_RS08625 (N4E_16840) - 1823427..1823729 (+) 303 WP_002368214.1 hypothetical protein -
  QUD80_RS08630 (N4E_16850) - 1823730..1824029 (+) 300 WP_002372047.1 MazG-like family protein -
  QUD80_RS08635 (N4E_16860) - 1824038..1824472 (+) 435 WP_002402491.1 RusA family crossover junction endodeoxyribonuclease -
  QUD80_RS08640 (N4E_16890) - 1825379..1825795 (+) 417 WP_002372045.1 ArpU family phage packaging/lysis transcriptional regulator -
  QUD80_RS08650 (N4E_16900) - 1826791..1827024 (+) 234 WP_002410538.1 hypothetical protein -
  QUD80_RS08655 (N4E_16910) - 1827168..1827746 (+) 579 WP_002370953.1 sce7726 family protein -
  QUD80_RS08660 (N4E_16920) - 1827784..1828701 (-) 918 WP_002370955.1 hypothetical protein -
  QUD80_RS08665 (N4E_16930) terS 1828970..1829773 (+) 804 WP_010816619.1 phage terminase small subunit -
  QUD80_RS08670 (N4E_16940) - 1829745..1831034 (+) 1290 WP_002393615.1 PBSX family phage terminase large subunit -
  QUD80_RS08675 (N4E_16950) - 1831046..1832533 (+) 1488 WP_002393614.1 phage portal protein -
  QUD80_RS08680 (N4E_16960) - 1832508..1834412 (+) 1905 WP_002393613.1 hypothetical protein -
  QUD80_RS08685 (N4E_16970) - 1834413..1834643 (+) 231 WP_002380437.1 hypothetical protein -
  QUD80_RS08690 (N4E_16980) - 1834700..1835020 (+) 321 WP_002380436.1 hypothetical protein -
  QUD80_RS08695 (N4E_16990) - 1835239..1835862 (+) 624 WP_002393977.1 DUF4355 domain-containing protein -
  QUD80_RS08700 (N4E_17000) - 1835876..1836763 (+) 888 WP_002357030.1 DUF5309 domain-containing protein -
  QUD80_RS08705 (N4E_17010) - 1836792..1836974 (+) 183 WP_002357032.1 hypothetical protein -
  QUD80_RS08710 (N4E_17020) - 1836988..1837332 (+) 345 WP_002357033.1 hypothetical protein -
  QUD80_RS08715 (N4E_17030) - 1837329..1837697 (+) 369 WP_002357034.1 hypothetical protein -
  QUD80_RS08720 (N4E_17040) - 1837690..1838088 (+) 399 WP_002357036.1 HK97 gp10 family phage protein -
  QUD80_RS08725 (N4E_17050) - 1838091..1838465 (+) 375 WP_002387286.1 DUF6838 family protein -
  QUD80_RS08730 (N4E_17060) - 1838466..1839314 (+) 849 WP_002387287.1 major tail protein -
  QUD80_RS08735 (N4E_17070) - 1839367..1839717 (+) 351 WP_002357039.1 hypothetical protein -
  QUD80_RS08740 (N4E_17080) - 1839965..1842862 (+) 2898 WP_002410539.1 tape measure protein -
  QUD80_RS08745 (N4E_17090) - 1842852..1843586 (+) 735 WP_002403868.1 tail protein -
  QUD80_RS08750 (N4E_17100) - 1843568..1846375 (+) 2808 WP_002410540.1 phage tail spike protein -
  QUD80_RS08755 (N4E_17110) - 1846394..1847275 (+) 882 WP_002387290.1 phage baseplate upper protein -
  QUD80_RS08760 (N4E_17120) - 1847268..1847864 (+) 597 WP_002357045.1 hypothetical protein -
  QUD80_RS08765 (N4E_17130) - 1847861..1848148 (+) 288 WP_002357046.1 collagen-like protein -
  QUD80_RS08770 (N4E_17140) - 1848148..1848639 (+) 492 WP_002364194.1 hypothetical protein -
  QUD80_RS08775 (N4E_17150) - 1848653..1848973 (+) 321 WP_002389262.1 hypothetical protein -
  QUD80_RS08780 (N4E_17160) - 1848975..1849130 (+) 156 WP_002364192.1 XkdX family protein -
  QUD80_RS08785 (N4E_17170) - 1849165..1849386 (+) 222 WP_002364191.1 hypothetical protein -
  QUD80_RS08790 (N4E_17180) - 1849379..1849612 (+) 234 WP_002384371.1 phage holin -
  QUD80_RS08795 (N4E_17190) - 1849613..1850854 (+) 1242 WP_002394567.1 LysM peptidoglycan-binding domain-containing protein -
  QUD80_RS08800 (N4E_17200) - 1851691..1851891 (+) 201 WP_002357053.1 cold-shock protein -
  QUD80_RS08805 (N4E_17210) - 1851963..1852214 (+) 252 WP_002364188.1 hypothetical protein -
  QUD80_RS08815 (N4E_17220) hemH 1852958..1853899 (+) 942 WP_033595884.1 ferrochelatase -
  QUD80_RS08820 (N4E_17230) - 1854196..1854321 (-) 126 WP_002364184.1 hypothetical protein -
  QUD80_RS08825 (N4E_17240) - 1854655..1855026 (+) 372 WP_002365830.1 type II secretion system protein -
  QUD80_RS08830 (N4E_17250) comGF 1855016..1855450 (+) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  QUD80_RS08835 (N4E_17260) comGG 1855450..1855803 (+) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  QUD80_RS08840 (N4E_17270) - 1855932..1856939 (+) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  QUD80_RS08845 (N4E_17280) - 1856964..1858151 (+) 1188 WP_002362052.1 acetate kinase -
  QUD80_RS08850 (N4E_17290) - 1858263..1858736 (-) 474 WP_002357065.1 universal stress protein -
  QUD80_RS08855 (N4E_17300) - 1859051..1859329 (-) 279 WP_002380425.1 hypothetical protein -
  QUD80_RS08865 (N4E_17310) - 1859657..1860934 (-) 1278 WP_174114184.1 replication-associated recombination protein A -
  QUD80_RS08870 (N4E_17320) - 1861063..1861740 (+) 678 WP_002364174.1 DNA-3-methyladenine glycosylase -
  QUD80_RS08875 (N4E_17330) - 1861697..1862182 (+) 486 WP_002388170.1 DUF3013 family protein -
  QUD80_RS08880 (N4E_17340) prmA 1862198..1863145 (+) 948 WP_002371999.1 50S ribosomal protein L11 methyltransferase -
  QUD80_RS08885 (N4E_17350) - 1863147..1863899 (+) 753 WP_002357078.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUD80_RS08890 (N4E_17360) - 1864114..1866327 (+) 2214 WP_002380422.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=100770 QUD80_RS08540 WP_002356991.1 1814341..1814616(+) (comGC/cglC) [Enterococcus faecalis strain N4E]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=100770 QUD80_RS08540 WP_002356991.1 1814341..1814616(+) (comGC/cglC) [Enterococcus faecalis strain N4E]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment