Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABKP85_RS12120 Genome accession   NZ_CP157379
Coordinates   2357250..2358230 (-) Length   326 a.a.
NCBI ID   WP_011836048.1    Uniprot ID   A2RNS9
Organism   Lactococcus cremoris strain NZ3900     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2349996..2363961 2357250..2358230 within 0


Gene organization within MGE regions


Location: 2349996..2363961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKP85_RS12065 (ABKP85_12030) - 2349996..2351391 (-) 1396 Protein_2351 IS3 family transposase -
  ABKP85_RS12070 (ABKP85_12035) - 2351464..2352273 (-) 810 WP_011836038.1 metal ABC transporter permease -
  ABKP85_RS12075 (ABKP85_12040) - 2352266..2353003 (-) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  ABKP85_RS12080 (ABKP85_12045) - 2353182..2354024 (-) 843 WP_011836040.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  ABKP85_RS12085 (ABKP85_12050) - 2354021..2354458 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  ABKP85_RS12090 (ABKP85_12055) comGG 2354539..2354838 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABKP85_RS12095 (ABKP85_12060) comGF 2354862..2355287 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABKP85_RS12100 (ABKP85_12065) comGE 2355271..2355567 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABKP85_RS12105 (ABKP85_12070) comGD 2355539..2355982 (-) 444 WP_011836045.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABKP85_RS12110 (ABKP85_12075) comGC 2355930..2356307 (-) 378 WP_011836046.1 competence type IV pilus major pilin ComGC Machinery gene
  ABKP85_RS12115 (ABKP85_12080) comGB 2356325..2357350 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABKP85_RS12120 (ABKP85_12085) comGA 2357250..2358230 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  ABKP85_RS12125 (ABKP85_12090) - 2358344..2362126 (-) 3783 Protein_2363 PolC-type DNA polymerase III -
  ABKP85_RS12130 (ABKP85_12095) - 2362658..2363251 (-) 594 Protein_2364 PolC-type DNA polymerase III N-terminal domain-containing protein -
  ABKP85_RS12135 (ABKP85_12100) - 2363425..2363961 (-) 537 WP_011836050.1 hypothetical protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36975.27 Da        Isoelectric Point: 5.9451

>NTDB_id=1006482 ABKP85_RS12120 WP_011836048.1 2357250..2358230(-) (comGA) [Lactococcus cremoris strain NZ3900]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEEVNQEIGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFAGPVGSGKTSLMFDLAHRHFSNAQV
ITIEEPVELIDSDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTVFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1006482 ABKP85_RS12120 WP_011836048.1 2357250..2358230(-) (comGA) [Lactococcus cremoris strain NZ3900]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAAGTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGGGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAACGAATCATTGGTTATCCGTCT
TCTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGATGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTGCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCACCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGATTCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACAGTTTTTTCTACTATTCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTAAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2RNS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

100

100

1
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

56.129

95.092

0.534
  comGA/cglA/cilD Streptococcus pneumoniae D39

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae R6

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

54.808

95.706

0.525
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.808

95.706

0.525
  comYA Streptococcus gordonii str. Challis substr. CH1

54.114

96.933

0.525
  comYA Streptococcus mutans UA140

52.412

95.399

0.5
  comYA Streptococcus mutans UA159

52.412

95.399

0.5

Multiple sequence alignment