Detailed information NTG1006478

Bioinformatically predicted

Overview

• Name: comGE
• Type: Machinery gene
• Locus tag: ABKP85_RS12100
• Genome accession: NZ_CP157379
• Coordinates: 2355271..2355567 (-)
• Length: 98 a.a.
• NCBI ID: WP_014735175.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain NZ3900
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	2349996..2363961	2355271..2355567	within	0

Gene organization within MGE regions

Location: 2349996..2363961

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABKP85_RS12065 (ABKP85_12030)	-	2349996..2351391 (-)	1396	Protein_2351	IS3 family transposase	-
ABKP85_RS12070 (ABKP85_12035)	-	2351464..2352273 (-)	810	WP_011836038.1	metal ABC transporter permease	-
ABKP85_RS12075 (ABKP85_12040)	-	2352266..2353003 (-)	738	WP_011836039.1	metal ABC transporter ATP-binding protein	-
ABKP85_RS12080 (ABKP85_12045)	-	2353182..2354024 (-)	843	WP_011836040.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
ABKP85_RS12085 (ABKP85_12050)	-	2354021..2354458 (-)	438	WP_011836041.1	zinc-dependent MarR family transcriptional regulator	-
ABKP85_RS12090 (ABKP85_12055)	comGG	2354539..2354838 (-)	300	WP_011836042.1	competence type IV pilus minor pilin ComGG	Machinery gene
ABKP85_RS12095 (ABKP85_12060)	comGF	2354862..2355287 (-)	426	WP_014735174.1	competence type IV pilus minor pilin ComGF	Machinery gene
ABKP85_RS12100 (ABKP85_12065)	comGE	2355271..2355567 (-)	297	WP_014735175.1	competence type IV pilus minor pilin ComGE	Machinery gene
ABKP85_RS12105 (ABKP85_12070)	comGD	2355539..2355982 (-)	444	WP_011836045.1	competence type IV pilus minor pilin ComGD	Machinery gene
ABKP85_RS12110 (ABKP85_12075)	comGC	2355930..2356307 (-)	378	WP_011836046.1	competence type IV pilus major pilin ComGC	Machinery gene
ABKP85_RS12115 (ABKP85_12080)	comGB	2356325..2357350 (-)	1026	WP_042211510.1	competence type IV pilus assembly protein ComGB	Machinery gene
ABKP85_RS12120 (ABKP85_12085)	comGA	2357250..2358230 (-)	981	WP_011836048.1	competence type IV pilus ATPase ComGA	Machinery gene
ABKP85_RS12125 (ABKP85_12090)	-	2358344..2362126 (-)	3783	Protein_2363	PolC-type DNA polymerase III	-
ABKP85_RS12130 (ABKP85_12095)	-	2362658..2363251 (-)	594	Protein_2364	PolC-type DNA polymerase III N-terminal domain-containing protein	-
ABKP85_RS12135 (ABKP85_12100)	-	2363425..2363961 (-)	537	WP_011836050.1	hypothetical protein	-

Sequence

Protein

• Length: 98 a.a.
• Molecular weight: 10976.61 Da
• Isoelectric Point: 5.0659

>NTDB_id=1006478 ABKP85_RS12100 WP_014735175.1 2355271..2355567(-) (comGE) [Lactococcus cremoris strain NZ3900]
MENIKRKSIKGYILLESLISMALLSFLVTFLLSSLTNSRQQEAQENQQIESLNVAQMAIESQLTELSLNGSVIKIRQDST
ATIISDHGKEILRLEAQN

Nucleotide

• Length: 297 bp

>NTDB_id=1006478 ABKP85_RS12100 WP_014735175.1 2355271..2355567(-) (comGE) [Lactococcus cremoris strain NZ3900]
GTGGAAAATATAAAAAGAAAATCAATTAAGGGATATATACTTCTTGAGAGCCTAATTAGTATGGCATTATTGAGCTTTTT
AGTCACTTTTCTCTTGAGTTCTCTTACTAATTCTCGTCAGCAAGAGGCACAAGAAAATCAACAAATTGAGTCCTTAAATG
TAGCTCAGATGGCAATAGAAAGTCAGTTGACGGAACTATCGTTAAATGGTTCTGTTATAAAAATAAGACAAGATTCGACT
GCAACCATTATTAGTGACCATGGAAAGGAAATTTTGCGACTTGAAGCTCAAAATTAG

Domains

Predicted by InterproScan.

• Type II secretory pathway pseudopilin (ComGE) (13-94)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGE	Lactococcus lactis subsp. cremoris KW2	100	100	1