Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ABKP85_RS12095 Genome accession   NZ_CP157379
Coordinates   2354862..2355287 (-) Length   141 a.a.
NCBI ID   WP_014735174.1    Uniprot ID   -
Organism   Lactococcus cremoris strain NZ3900     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2349996..2363961 2354862..2355287 within 0


Gene organization within MGE regions


Location: 2349996..2363961
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKP85_RS12065 (ABKP85_12030) - 2349996..2351391 (-) 1396 Protein_2351 IS3 family transposase -
  ABKP85_RS12070 (ABKP85_12035) - 2351464..2352273 (-) 810 WP_011836038.1 metal ABC transporter permease -
  ABKP85_RS12075 (ABKP85_12040) - 2352266..2353003 (-) 738 WP_011836039.1 metal ABC transporter ATP-binding protein -
  ABKP85_RS12080 (ABKP85_12045) - 2353182..2354024 (-) 843 WP_011836040.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  ABKP85_RS12085 (ABKP85_12050) - 2354021..2354458 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  ABKP85_RS12090 (ABKP85_12055) comGG 2354539..2354838 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABKP85_RS12095 (ABKP85_12060) comGF 2354862..2355287 (-) 426 WP_014735174.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABKP85_RS12100 (ABKP85_12065) comGE 2355271..2355567 (-) 297 WP_014735175.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABKP85_RS12105 (ABKP85_12070) comGD 2355539..2355982 (-) 444 WP_011836045.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABKP85_RS12110 (ABKP85_12075) comGC 2355930..2356307 (-) 378 WP_011836046.1 competence type IV pilus major pilin ComGC Machinery gene
  ABKP85_RS12115 (ABKP85_12080) comGB 2356325..2357350 (-) 1026 WP_042211510.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABKP85_RS12120 (ABKP85_12085) comGA 2357250..2358230 (-) 981 WP_011836048.1 competence type IV pilus ATPase ComGA Machinery gene
  ABKP85_RS12125 (ABKP85_12090) - 2358344..2362126 (-) 3783 Protein_2363 PolC-type DNA polymerase III -
  ABKP85_RS12130 (ABKP85_12095) - 2362658..2363251 (-) 594 Protein_2364 PolC-type DNA polymerase III N-terminal domain-containing protein -
  ABKP85_RS12135 (ABKP85_12100) - 2363425..2363961 (-) 537 WP_011836050.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 16210.78 Da        Isoelectric Point: 8.4549

>NTDB_id=1006477 ABKP85_RS12095 WP_014735174.1 2354862..2355287(-) (comGF) [Lactococcus cremoris strain NZ3900]
MKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFLYVTKDKK
LRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1006477 ABKP85_RS12095 WP_014735174.1 2354862..2355287(-) (comGF) [Lactococcus cremoris strain NZ3900]
TTGAAGCTCAAAATTAGGGCATTTACTTTACTAGAATGTCTCGTAGCACTTTTGGCAATTTCTGGTTCAGTGCTAGTCAT
CTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGAAAGATTGGCAAATATTTTGTG
AACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTGTATGTGACCAAAGATAAGAAG
TTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATACCAACCGATGCTTTATGATTT
AAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATAATGGAGGTGAGCGAGTATTTA
TTTATCGATTTACAGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(38-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

100

100

1
  comYF Streptococcus mutans UA140

43.972

100

0.44
  comYF Streptococcus mutans UA159

43.262

100

0.433
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

99.291

0.39
  comGF/cglF Streptococcus mitis SK321

39.286

99.291

0.39
  comGF/cglF Streptococcus pneumoniae Rx1

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae D39

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae R6

37.857

99.291

0.376
  comGF/cglF Streptococcus pneumoniae TIGR4

37.857

99.291

0.376

Multiple sequence alignment