Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ABKA22_RS09950 Genome accession   NZ_CP157287
Coordinates   1996061..1996486 (-) Length   141 a.a.
NCBI ID   WP_348618883.1    Uniprot ID   -
Organism   Lactococcus lactis strain BIM B-1834     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1960687..2007433 1996061..1996486 within 0


Gene organization within MGE regions


Location: 1960687..2007433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKA22_RS09710 (ABKA22_09710) pknB 1960687..1962570 (-) 1884 WP_348618816.1 Stk1 family PASTA domain-containing Ser/Thr kinase -
  ABKA22_RS09715 (ABKA22_09715) - 1962570..1963346 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  ABKA22_RS09720 (ABKA22_09720) rsmB 1963427..1964701 (-) 1275 WP_014570715.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  ABKA22_RS09730 (ABKA22_09730) - 1965136..1965372 (-) 237 WP_011834818.1 bacteriophage holin -
  ABKA22_RS09735 (ABKA22_09735) - 1965564..1965947 (-) 384 WP_029344147.1 hypothetical protein -
  ABKA22_RS09740 (ABKA22_09740) - 1965947..1966399 (-) 453 WP_230579356.1 type II toxin-antitoxin system antitoxin SocA domain-containing protein -
  ABKA22_RS09745 (ABKA22_09745) - 1966610..1967389 (-) 780 WP_348618820.1 peptidoglycan amidohydrolase family protein -
  ABKA22_RS09750 (ABKA22_09750) - 1967389..1967676 (-) 288 WP_348618822.1 phage holin -
  ABKA22_RS09755 (ABKA22_09755) - 1967702..1969264 (-) 1563 WP_348618824.1 BppU family phage baseplate upper protein -
  ABKA22_RS09760 (ABKA22_09760) - 1969276..1969500 (-) 225 WP_010905920.1 hemolysin XhlA family protein -
  ABKA22_RS09765 (ABKA22_09765) - 1969624..1971909 (-) 2286 WP_348618827.1 BppU family phage baseplate upper protein -
  ABKA22_RS09770 (ABKA22_09770) - 1971931..1974747 (-) 2817 WP_348618829.1 phage tail spike protein -
  ABKA22_RS09775 (ABKA22_09775) - 1974747..1975508 (-) 762 WP_348618831.1 distal tail protein Dit -
  ABKA22_RS09780 (ABKA22_09780) - 1975522..1978335 (-) 2814 WP_348618833.1 phage tail tape measure protein -
  ABKA22_RS09785 (ABKA22_09785) - 1978350..1978613 (-) 264 WP_043994770.1 hypothetical protein -
  ABKA22_RS09790 (ABKA22_09790) - 1978709..1979044 (-) 336 WP_015966829.1 tail assembly chaperone -
  ABKA22_RS09795 (ABKA22_09795) - 1979160..1979657 (-) 498 WP_267165180.1 phage major tail protein, TP901-1 family -
  ABKA22_RS09800 (ABKA22_09800) - 1979668..1980063 (-) 396 WP_348618836.1 phage capsid protein -
  ABKA22_RS09805 (ABKA22_09805) - 1980060..1980398 (-) 339 WP_014570728.1 HK97-gp10 family putative phage morphogenesis protein -
  ABKA22_RS09810 (ABKA22_09810) - 1980395..1980706 (-) 312 WP_032398561.1 hypothetical protein -
  ABKA22_RS09815 (ABKA22_09815) - 1980703..1981035 (-) 333 WP_348618839.1 phage head-tail connector protein -
  ABKA22_RS09820 (ABKA22_09820) - 1981022..1981219 (-) 198 WP_014570729.1 HeH/LEM domain-containing protein -
  ABKA22_RS09825 (ABKA22_09825) - 1981219..1982046 (-) 828 WP_348619249.1 N4-gp56 family major capsid protein -
  ABKA22_RS09830 (ABKA22_09830) - 1982042..1982737 (-) 696 WP_348618842.1 DUF4355 domain-containing protein -
  ABKA22_RS09835 (ABKA22_09835) - 1982861..1983040 (-) 180 WP_348618844.1 hypothetical protein -
  ABKA22_RS09840 (ABKA22_09840) - 1983099..1983431 (-) 333 WP_348618846.1 YjcQ family protein -
  ABKA22_RS09845 (ABKA22_09845) - 1983477..1984517 (-) 1041 WP_348618848.1 minor capsid protein -
  ABKA22_RS09850 (ABKA22_09850) - 1984521..1985891 (-) 1371 WP_348618850.1 phage portal protein -
  ABKA22_RS09855 (ABKA22_09855) terL 1985904..1987292 (-) 1389 WP_348618852.1 phage terminase large subunit -
  ABKA22_RS09860 (ABKA22_09860) - 1987285..1987695 (-) 411 WP_011676523.1 terminase -
  ABKA22_RS09865 (ABKA22_09865) - 1987932..1988354 (-) 423 WP_260311634.1 RinA family protein -
  ABKA22_RS09870 (ABKA22_09870) - 1989007..1989189 (-) 183 WP_010905705.1 hypothetical protein -
  ABKA22_RS09875 (ABKA22_09875) - 1989186..1989485 (-) 300 WP_348618855.1 hypothetical protein -
  ABKA22_RS09880 (ABKA22_09880) - 1989486..1989701 (-) 216 WP_348618857.1 DUF1660 domain-containing protein -
  ABKA22_RS09885 (ABKA22_09885) - 1989698..1989916 (-) 219 WP_348618859.1 hypothetical protein -
  ABKA22_RS09890 (ABKA22_09890) - 1989963..1990655 (-) 693 WP_348618864.1 hypothetical protein -
  ABKA22_RS09895 (ABKA22_09895) - 1990652..1991002 (-) 351 WP_348618866.1 hypothetical protein -
  ABKA22_RS09900 (ABKA22_09900) dut 1991005..1991424 (-) 420 WP_348618868.1 dUTP diphosphatase -
  ABKA22_RS09905 (ABKA22_09905) - 1991421..1991990 (-) 570 WP_348618870.1 DUF1642 domain-containing protein -
  ABKA22_RS09910 (ABKA22_09910) - 1991983..1992189 (-) 207 WP_011676933.1 DUF1125 domain-containing protein -
  ABKA22_RS09915 (ABKA22_09915) - 1992203..1992727 (-) 525 WP_348618872.1 hypothetical protein -
  ABKA22_RS09920 (ABKA22_09920) - 1992836..1993075 (-) 240 WP_348618874.1 DUF1031 family protein -
  ABKA22_RS09925 (ABKA22_09925) - 1993072..1993377 (-) 306 WP_348618876.1 hypothetical protein -
  ABKA22_RS09930 (ABKA22_09930) - 1993382..1993771 (-) 390 WP_348618878.1 RusA family crossover junction endodeoxyribonuclease -
  ABKA22_RS09935 (ABKA22_09935) dcm 1993768..1994880 (-) 1113 WP_289452664.1 DNA (cytosine-5-)-methyltransferase -
  ABKA22_RS09940 (ABKA22_09940) - 1994867..1995142 (-) 276 WP_336255775.1 hypothetical protein -
  ABKA22_RS09945 (ABKA22_09945) - 1995126..1995932 (-) 807 WP_348618881.1 helix-turn-helix domain-containing protein -
  ABKA22_RS09950 (ABKA22_09950) ssb 1996061..1996486 (-) 426 WP_348618883.1 single-stranded DNA-binding protein Machinery gene
  ABKA22_RS09955 (ABKA22_09955) - 1996479..1997237 (-) 759 WP_348618885.1 Rad52/Rad22 family DNA repair protein -
  ABKA22_RS09960 (ABKA22_09960) - 1997246..1997641 (-) 396 WP_348618887.1 hypothetical protein -
  ABKA22_RS09965 (ABKA22_09965) - 1997740..1997913 (-) 174 WP_014573167.1 putative transcriptional regulator -
  ABKA22_RS09970 (ABKA22_09970) - 1997944..1998180 (-) 237 WP_259743211.1 DUF1408 domain-containing protein -
  ABKA22_RS09975 (ABKA22_09975) - 1998284..1998784 (+) 501 WP_101913573.1 hypothetical protein -
  ABKA22_RS09980 (ABKA22_09980) - 1998762..1999010 (-) 249 WP_348618890.1 hypothetical protein -
  ABKA22_RS09985 (ABKA22_09985) - 1999105..1999374 (-) 270 WP_023164536.1 hypothetical protein -
  ABKA22_RS09990 (ABKA22_09990) - 1999387..2000175 (-) 789 WP_312257963.1 phage antirepressor KilAC domain-containing protein -
  ABKA22_RS09995 (ABKA22_09995) - 2000188..2000427 (-) 240 WP_023189646.1 helix-turn-helix transcriptional regulator -
  ABKA22_RS10000 (ABKA22_10000) - 2000581..2001378 (+) 798 WP_348618893.1 hypothetical protein -
  ABKA22_RS10005 (ABKA22_10005) - 2001398..2001568 (-) 171 WP_058224702.1 putative transcriptional regulator -
  ABKA22_RS10010 (ABKA22_10010) - 2001843..2002406 (+) 564 WP_014570764.1 helix-turn-helix transcriptional regulator -
  ABKA22_RS10015 (ABKA22_10015) - 2002412..2002846 (+) 435 WP_031559266.1 zinc peptidase -
  ABKA22_RS10020 (ABKA22_10020) - 2002860..2003195 (+) 336 WP_031559267.1 hypothetical protein -
  ABKA22_RS10025 (ABKA22_10025) - 2003262..2004443 (+) 1182 WP_251904598.1 site-specific integrase -
  ABKA22_RS10030 (ABKA22_10030) - 2004743..2005249 (-) 507 WP_003131479.1 Asp23/Gls24 family envelope stress response protein -
  ABKA22_RS10035 (ABKA22_10035) - 2005270..2005458 (-) 189 WP_012898445.1 DUF2273 domain-containing protein -
  ABKA22_RS10040 (ABKA22_10040) amaP 2005469..2006029 (-) 561 WP_010906186.1 alkaline shock response membrane anchor protein AmaP -
  ABKA22_RS10045 (ABKA22_10045) - 2006057..2006299 (-) 243 WP_003131476.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  ABKA22_RS10050 (ABKA22_10050) fmt 2006474..2007433 (-) 960 WP_251906059.1 methionyl-tRNA formyltransferase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15712.44 Da        Isoelectric Point: 5.1972

>NTDB_id=1005446 ABKA22_RS09950 WP_348618883.1 1996061..1996486(-) (ssb) [Lactococcus lactis strain BIM B-1834]
MINNVTLVGRITKEPELRYTPQNKAVASFTLAVNRQFKNANGEREADFINCVIWGKSAENLANWTHKGQLIGVIGNIQTR
NYENQQGQRVYVTEVVASNFQVLEKSNQANGERVGNPAAKPQNNDSFGSDPMEISDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1005446 ABKA22_RS09950 WP_348618883.1 1996061..1996486(-) (ssb) [Lactococcus lactis strain BIM B-1834]
ATGATTAACAATGTCACTCTAGTAGGAAGAATCACTAAAGAACCTGAACTTAGATATACACCACAAAATAAAGCAGTTGC
TTCATTTACTCTTGCAGTTAATCGTCAATTTAAAAATGCTAATGGAGAAAGAGAAGCTGACTTCATCAATTGTGTTATCT
GGGGTAAATCAGCCGAAAACTTGGCCAATTGGACTCATAAAGGTCAATTAATTGGAGTTATTGGGAATATCCAAACTCGA
AACTATGAGAACCAACAAGGGCAACGTGTTTATGTTACGGAGGTTGTCGCAAGTAATTTCCAAGTACTAGAAAAAAGTAA
TCAAGCAAATGGTGAACGAGTTGGTAATCCAGCTGCAAAACCACAAAATAACGATTCTTTTGGAAGTGATCCAATGGAAA
TTTCAGATGATGACCTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

55.294

100

0.667

  ssbA Bacillus subtilis subsp. subtilis str. 168

52.326

100

0.638

  ssbB/cilA Streptococcus pneumoniae TIGR4

44.681

100

0.447

  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.681

100

0.447

  ssbB/cilA Streptococcus mitis NCTC 12261

44.681

100

0.447

  ssbB/cilA Streptococcus pneumoniae Rx1

43.972

100

0.44

  ssbB/cilA Streptococcus pneumoniae D39

43.972

100

0.44

  ssbB/cilA Streptococcus pneumoniae R6

43.972

100

0.44

  ssbB/cilA Streptococcus mitis SK321

43.972

100

0.44

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.654

73.759

0.433

  ssbA Streptococcus mutans UA159

40.426

100

0.404

  ssbB Lactococcus lactis subsp. cremoris KW2

49.515

73.05

0.362


Multiple sequence alignment