Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMG27_RS10890 Genome accession   NZ_AP027058
Coordinates   2278334..2279551 (+) Length   405 a.a.
NCBI ID   WP_281811268.1    Uniprot ID   -
Organism   Limnohabitans sp. MORI2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2273334..2284551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG27_RS10850 (LMORI2_21250) cgtA 2273769..2274839 (-) 1071 WP_281811255.1 Obg family GTPase CgtA -
  QMG27_RS10855 (LMORI2_21260) rpmA 2274904..2275161 (-) 258 WP_104796974.1 50S ribosomal protein L27 -
  QMG27_RS10860 (LMORI2_21270) rplU 2275174..2275485 (-) 312 WP_281811257.1 50S ribosomal protein L21 -
  QMG27_RS10865 (LMORI2_21280) - 2275677..2276606 (+) 930 WP_281814621.1 polyprenyl synthetase family protein -
  QMG27_RS10870 (LMORI2_21290) - 2276625..2277026 (+) 402 WP_281811259.1 hypothetical protein -
  QMG27_RS10875 (LMORI2_21300) - 2277044..2277370 (-) 327 WP_281811261.1 hypothetical protein -
  QMG27_RS10880 (LMORI2_21310) - 2277396..2277776 (-) 381 WP_281811263.1 H-NS histone family protein -
  QMG27_RS10885 (LMORI2_21320) - 2277888..2278160 (-) 273 WP_281811266.1 HU family DNA-binding protein -
  QMG27_RS10890 (LMORI2_21330) pilB 2278334..2279551 (+) 1218 WP_281811268.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  QMG27_RS10895 (LMORI2_21340) - 2279544..2280758 (+) 1215 WP_281811271.1 type II secretion system F family protein -
  QMG27_RS10900 - 2280755..2281051 (+) 297 Protein_2143 prepilin peptidase -
  QMG27_RS10905 (LMORI2_21350) - 2281055..2281558 (+) 504 WP_281814624.1 A24 family peptidase -
  QMG27_RS10910 (LMORI2_21360) coaE 2281580..2282197 (+) 618 WP_281811273.1 dephospho-CoA kinase -
  QMG27_RS10915 (LMORI2_21370) zapD 2282270..2283022 (+) 753 WP_281811274.1 cell division protein ZapD -
  QMG27_RS10920 (LMORI2_21380) - 2283026..2283226 (+) 201 WP_281811277.1 DNA gyrase inhibitor YacG -
  QMG27_RS10925 (LMORI2_21390) mutT 2283241..2283711 (-) 471 WP_281811279.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44557.18 Da        Isoelectric Point: 6.7759

>NTDB_id=99448 QMG27_RS10890 WP_281811268.1 2278334..2279551(+) (pilB) [Limnohabitans sp. MORI2]
MSQRECRIPMPTDNAVNTLDDLLQQALLQGASDVHFESGEDFFRARFRIDGILRVAATPALTLRDAVVSRIKVLARMDIA
EKRLPQDGRIQYPHLGQTVDLRISSLPTVYGEKIVVRILNYSRERPGLAALGYEPDERAKLIQALGQPHGLILMTGPTGS
GKTLSLYSCLELLNRHEINISTVEDPSEIHLPGANQVNINERAGLTFASALRALLRQDPDVIMVGEIRDLETAEIAIQAA
QTGHLVLSTLHTNDATGTLARLRHMGIAAFNVAASVSLVTAQRLVRRLCEHCKQAMDASETEFLLSNLNDQDLRTIPAAL
RSQAMPHKAIGCALCDQGYKGRIGIYQVLPISHEMQALIMRDSDTHALAAQATHEGVRTLRQAGWVKVMQGLTSMEEIMA
LTHHD

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=99448 QMG27_RS10890 WP_281811268.1 2278334..2279551(+) (pilB) [Limnohabitans sp. MORI2]
TTGTCCCAACGCGAGTGCCGTATACCCATGCCGACTGACAACGCCGTCAACACACTAGACGACTTACTGCAACAAGCGCT
ACTTCAAGGTGCATCGGATGTTCACTTTGAAAGTGGTGAAGATTTCTTTCGCGCACGATTTCGCATTGATGGCATCCTGC
GGGTAGCAGCAACGCCTGCGCTGACACTCAGAGATGCCGTCGTATCTCGCATCAAAGTTTTAGCTCGTATGGACATTGCT
GAAAAACGGCTGCCTCAAGACGGACGAATTCAATATCCCCATCTGGGACAAACCGTGGATTTGCGCATTAGCTCATTGCC
GACTGTTTATGGGGAAAAAATTGTCGTCCGCATACTCAACTACAGCCGTGAACGTCCTGGTTTGGCCGCTCTGGGTTATG
AACCTGATGAACGCGCCAAGTTAATACAAGCCCTCGGACAGCCTCACGGCTTAATTTTGATGACAGGACCAACCGGCTCA
GGCAAAACCTTATCTTTGTATAGCTGCCTTGAGTTGCTCAATCGTCATGAAATCAACATTTCCACAGTTGAAGACCCTTC
GGAAATTCACCTTCCGGGAGCCAATCAGGTCAACATCAATGAACGTGCAGGGTTGACATTTGCAAGTGCACTTCGTGCGC
TTTTACGTCAAGACCCAGACGTGATCATGGTGGGTGAAATACGCGATCTGGAAACCGCAGAAATCGCCATCCAAGCTGCA
CAAACAGGGCATTTGGTTTTATCCACCTTGCACACCAACGATGCCACTGGGACATTGGCCCGTTTGCGGCATATGGGCAT
CGCAGCATTCAATGTTGCAGCCAGCGTGAGTCTGGTGACAGCTCAGCGCTTAGTGCGTCGTTTGTGTGAGCATTGCAAGC
AAGCGATGGATGCGTCAGAAACCGAGTTTCTCTTGAGTAATTTAAATGACCAAGATCTGCGAACGATCCCAGCGGCTCTG
CGCTCGCAAGCGATGCCACACAAAGCCATAGGTTGCGCATTGTGTGACCAAGGTTACAAAGGACGTATTGGCATTTATCA
GGTTCTTCCGATCAGCCATGAGATGCAAGCACTGATCATGCGAGACAGTGATACTCACGCCTTGGCTGCTCAAGCCACGC
ATGAAGGTGTACGGACACTGCGCCAAGCAGGGTGGGTCAAGGTCATGCAAGGCCTCACATCCATGGAAGAAATCATGGCG
CTGACGCACCATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(22-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.093

95.802

0.509
  pilF Neisseria gonorrhoeae MS11

52.727

95.062

0.501
  pilB Acinetobacter baumannii D1279779

51.531

96.79

0.499
  pilB Legionella pneumophila strain ERS1305867

49.114

97.531

0.479
  pilB Vibrio cholerae strain A1552

49.869

94.074

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

47.781

94.568

0.452
  pilB Vibrio campbellii strain DS40M4

47.906

94.321

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

43.86

98.519

0.432
  pilF Thermus thermophilus HB27

42.365

100

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.445

96.049

0.417
  pilB Haemophilus influenzae 86-028NP

42.338

95.062

0.402
  pilB Haemophilus influenzae Rd KW20

42.338

95.062

0.402
  pilB Glaesserella parasuis strain SC1401

40.206

95.802

0.385
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.791

94.321

0.375

Multiple sequence alignment