Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8775_RS09730 Genome accession   NZ_AP026915
Coordinates   1853592..1854608 (-) Length   338 a.a.
NCBI ID   WP_078134945.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700005     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1848592..1859608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8775_RS09685 - 1848669..1848800 (-) 132 WP_000768904.1 hypothetical protein -
  R8775_RS09690 (PC0005_18800) - 1848801..1849991 (-) 1191 WP_050233485.1 acetate kinase -
  R8775_RS09695 (PC0005_18810) comYH 1850042..1850995 (-) 954 WP_050233486.1 class I SAM-dependent methyltransferase Machinery gene
  R8775_RS09700 - 1851056..1851650 (-) 595 Protein_1889 class I SAM-dependent methyltransferase -
  R8775_RS09705 (PC0005_18840) comGG/cglG 1851787..1852200 (-) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8775_RS09710 comGF/cglF 1852178..1852639 (-) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8775_RS09715 (PC0005_18860) comGE/cglE 1852602..1852904 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8775_RS09720 (PC0005_18870) comGD/cglD 1852867..1853271 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8775_RS09725 (PC0005_18880) comGC/cglC 1853264..1853590 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  R8775_RS09730 (PC0005_18890) comGB/cglB 1853592..1854608 (-) 1017 WP_078134945.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8775_RS09735 (PC0005_18900) comGA/cglA/cilD 1854556..1855497 (-) 942 WP_000249569.1 competence type IV pilus ATPase ComGA Machinery gene
  R8775_RS09740 (PC0005_18910) - 1855573..1855938 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8775_RS09745 (PC0005_18920) - 1856089..1857147 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R8775_RS09750 (PC0005_18930) nagA 1857310..1858461 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38424.39 Da        Isoelectric Point: 9.4802

>NTDB_id=97600 R8775_RS09730 WP_078134945.1 1853592..1854608(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700005]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=97600 R8775_RS09730 WP_078134945.1 1853592..1854608(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700005]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGATAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae D39

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae R6

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae TIGR4

99.112

100

0.991
  comGB/cglB Streptococcus mitis SK321

95.562

100

0.956
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment