Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AABM55_RS03775 Genome accession   NZ_CP150917
Coordinates   856289..857506 (-) Length   405 a.a.
NCBI ID   WP_054595546.1    Uniprot ID   A0A2N8BX06
Organism   Pseudomonas helvetica strain DGS28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851289..862506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM55_RS03740 - 852198..852437 (-) 240 WP_347928860.1 DUF2790 domain-containing protein -
  AABM55_RS03745 - 852559..853491 (+) 933 WP_347928861.1 LysR family transcriptional regulator -
  AABM55_RS03750 - 853622..854308 (+) 687 WP_347928862.1 energy-coupling factor ABC transporter permease -
  AABM55_RS03755 - 854372..854587 (+) 216 WP_054595542.1 hypothetical protein -
  AABM55_RS03760 yacG 854597..854797 (-) 201 WP_054595543.1 DNA gyrase inhibitor YacG -
  AABM55_RS03765 coaE 854794..855417 (-) 624 WP_054595544.1 dephospho-CoA kinase -
  AABM55_RS03770 pilD 855414..856286 (-) 873 WP_347928863.1 A24 family peptidase Machinery gene
  AABM55_RS03775 pilC 856289..857506 (-) 1218 WP_054595546.1 type II secretion system F family protein Machinery gene
  AABM55_RS03780 pilB 857510..859210 (-) 1701 WP_347928864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABM55_RS03785 - 859437..859847 (+) 411 WP_054595548.1 pilin -
  AABM55_RS03790 - 860125..860481 (+) 357 WP_347928865.1 BON domain-containing protein -
  AABM55_RS03795 - 860593..860892 (-) 300 WP_054595550.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44269.31 Da        Isoelectric Point: 10.0044

>NTDB_id=973748 AABM55_RS03775 WP_054595546.1 856289..857506(-) (pilC) [Pseudomonas helvetica strain DGS28]
MAVKAIKVSVYAWEGTDRKGSKVTGELSGHNPALIKAQLRKQGINPGKVRKKTASLFNLGNRIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQAGALDTLLERIATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFQSVFSGFGAELPAFTLMVIGLSEFMQAWWWLLLGGLIIGALSFR
HALKRSQRFCDWLDAKLLKMPILGALLYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSRIFPNMAIQMAAIGEESGALDDMLDKVAIYYEAEVDNMVDSLTSLLEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=973748 AABM55_RS03775 WP_054595546.1 856289..857506(-) (pilC) [Pseudomonas helvetica strain DGS28]
ATGGCGGTCAAAGCAATCAAGGTCAGCGTCTACGCCTGGGAAGGCACCGACAGGAAAGGCAGCAAGGTAACCGGCGAGCT
GAGCGGTCATAACCCGGCCTTGATCAAGGCGCAGCTACGCAAACAAGGGATCAACCCGGGCAAAGTACGGAAAAAAACCG
CTTCGCTGTTCAACCTCGGCAACCGTATCAAGCCGCTGGACATTGCTCTCTTCACCCGGCAAATGGCGACCATGATGAAA
GCCGGTGTGCCGCTGCTGCAGTCGTTCGACATCATCGGCGAGGGCTTCGACAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTCGCCACCGCGCTGCGTAAAAAACCACAGTACTTCGATGAGCTCTACT
GCAACCTGGTCGACGCGGGCGAGCAGGCCGGCGCGCTCGACACGCTGCTGGAGCGGATTGCGACCTACAAGGAGAAAAGC
GAAAGCCTCAAGGCCAAGATCAAAAAAGCCATGACCTATCCGCTGGCCGTGGTCTTTGTCGCAATCATCGTCACCGGCAT
TCTGCTGGTCAAAGTGGTCCCGCAATTTCAGTCGGTGTTTTCCGGATTTGGTGCAGAACTCCCGGCCTTTACCCTGATGG
TGATCGGTCTGTCCGAGTTCATGCAGGCGTGGTGGTGGCTGTTACTCGGCGGCCTGATCATCGGGGCCCTGAGCTTTCGC
CATGCGCTCAAGCGCTCGCAACGCTTTTGCGACTGGCTGGATGCCAAACTGCTGAAAATGCCGATACTCGGCGCACTGCT
CTACAAATCGGCGGTGGCGCGCTTTGCCCGTACCCTGTCGACCACCTTCGCCGCAGGCGTCCCCCTGGTCGAAGCTCTGG
ATTCGGTAGCAGGTGCTACTGGCAATATCGTGTTCAAACGGGCGGTTCTGCGCATCAAACAGGATGTCTCCACCGGCATG
CAATTGAACTTCTCGATGCGCACCAGCAGGATTTTCCCCAACATGGCGATTCAAATGGCCGCCATTGGCGAGGAGTCTGG
CGCACTCGACGATATGCTCGACAAAGTTGCGATCTACTATGAAGCTGAAGTCGACAATATGGTCGACAGCCTGACCAGCC
TGTTGGAGCCGTTCATCATGGTGGTGCTCGGCGTCATTGTCGGCGGGCTGGTGGTTGCCATGTACCTGCCCATTTTCCAA
CTCGGCTCAGCGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8BX06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

60.244

100

0.61
  pilC Acinetobacter baumannii D1279779

58.088

100

0.585
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilC Vibrio cholerae strain A1552

42.402

100

0.427
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

42.68

99.506

0.425
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment