Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AABM55_RS03780 Genome accession   NZ_CP150917
Coordinates   857510..859210 (-) Length   566 a.a.
NCBI ID   WP_347928864.1    Uniprot ID   -
Organism   Pseudomonas helvetica strain DGS28     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852510..864210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABM55_RS03745 - 852559..853491 (+) 933 WP_347928861.1 LysR family transcriptional regulator -
  AABM55_RS03750 - 853622..854308 (+) 687 WP_347928862.1 energy-coupling factor ABC transporter permease -
  AABM55_RS03755 - 854372..854587 (+) 216 WP_054595542.1 hypothetical protein -
  AABM55_RS03760 yacG 854597..854797 (-) 201 WP_054595543.1 DNA gyrase inhibitor YacG -
  AABM55_RS03765 coaE 854794..855417 (-) 624 WP_054595544.1 dephospho-CoA kinase -
  AABM55_RS03770 pilD 855414..856286 (-) 873 WP_347928863.1 A24 family peptidase Machinery gene
  AABM55_RS03775 pilC 856289..857506 (-) 1218 WP_054595546.1 type II secretion system F family protein Machinery gene
  AABM55_RS03780 pilB 857510..859210 (-) 1701 WP_347928864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AABM55_RS03785 - 859437..859847 (+) 411 WP_054595548.1 pilin -
  AABM55_RS03790 - 860125..860481 (+) 357 WP_347928865.1 BON domain-containing protein -
  AABM55_RS03795 - 860593..860892 (-) 300 WP_054595550.1 DUF2845 domain-containing protein -
  AABM55_RS03800 - 860895..863837 (-) 2943 WP_347928866.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62716.72 Da        Isoelectric Point: 6.1575

>NTDB_id=973749 AABM55_RS03780 WP_347928864.1 857510..859210(-) (pilB) [Pseudomonas helvetica strain DGS28]
MNDIALSGLAKQLVLAELLTDKSAQQAYQQAQRNRVSLVSYLVQNKMVSSRQVAEIASEHFGVAFLDLNCLDKDSQPKDL
VSEKLIRQHHALPLWRRGNKLFVGISDPSNQQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFDSHSTGLEDMADIDLGG
LDIKAVDDQRQDAVTGQDSDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPTQLANRIAARL
KVMASLDISERRKPQDGRLKMHLSKSKSIDFRVNTLPTLWGEKIVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATAVHLIIAQRLARKLCPHCKKNLSIPDETLIKEGFPR
ERIGSFTIYEPVGCDLCNNGYKGRVGIYEVVKNTPQLQRLIMAEGNSLEIDTQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=973749 AABM55_RS03780 WP_347928864.1 857510..859210(-) (pilB) [Pseudomonas helvetica strain DGS28]
ATGAATGACATCGCCCTCAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGCTCACTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCTCAGCGCAATCGCGTTTCCCTGGTCAGCTATCTGGTACAGAACAAAATGGTGAGCAGTCGCCAGGTTG
CGGAAATTGCCTCAGAGCATTTCGGCGTCGCCTTCCTCGACCTCAACTGTCTCGACAAAGACTCCCAGCCCAAAGACCTG
GTCAGTGAAAAGCTGATTCGCCAGCACCACGCCCTGCCACTCTGGCGGCGCGGCAACAAACTGTTCGTGGGCATTTCCGA
CCCGAGCAATCAGCAGGCGATCAACGATATCCAGTTCAGCACCGGGCTCACCACCGAAGCGATCCTGGTCGAAGACGACA
AGCTCAGCGACGCCATCGAGAAATTCTTCGACAGCCACAGCACGGGCCTGGAAGACATGGCCGATATCGACCTCGGGGGC
CTGGACATCAAAGCCGTCGACGACCAGCGCCAGGACGCTGTCACCGGCCAGGACTCCGACGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCCTCGGACCTGCATTTCGAGCCCTACGAAAAAATCTATCGGG
TGCGGGTACGGACCGACGGCATGCTGCGCGAAGTTGCCAGGCCGCCGACTCAATTGGCCAACCGCATCGCTGCCCGTCTC
AAGGTGATGGCCAGCCTGGACATTTCGGAACGTCGAAAACCCCAGGACGGGCGGCTCAAGATGCACCTGTCGAAAAGCAA
ATCCATCGATTTTCGGGTCAACACCCTGCCAACGTTGTGGGGCGAAAAAATCGTGATACGGATTCTCGACCCCTCCAGTG
CACAGATGGGCATCGACGCGCTGGGTTACGAGCCCGATCAAAAAGACCTGTATATGGCGGCGCTCAAGCAACCTCAAGGG
CTGATTCTGGTCACCGGGCCGACGGGTTCAGGGAAAACCGTGTCGCTGTACACCGGGTTGAATATCCTTAATACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTAGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAGGCAGG
GCCTGGATTTCGCTCAGGCATTGCGCTCATTTCTGCGCCAGGACCCGGACGTGATAATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAAATCGCGATCAAGGCTGCGCAAACCGGACACCTGGTGCTTTCCACCCTGCACACCAACAGCGCGGCTGA
AACCCTGACCCGGCTGCACAACATGGGCATCCCGGGGTTCAACATCGCCACGGCCGTGCACCTGATCATTGCCCAGCGCC
TGGCCCGCAAGCTGTGCCCCCACTGCAAAAAAAACCTCTCGATTCCCGACGAAACTCTGATCAAGGAAGGCTTCCCACGG
GAACGCATCGGCTCCTTCACGATCTACGAACCCGTCGGTTGCGATCTCTGCAACAACGGCTACAAAGGGCGAGTCGGAAT
CTATGAAGTGGTGAAAAACACCCCGCAGCTGCAACGGCTGATCATGGCTGAAGGCAATTCTCTGGAAATCGATACGCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACCTCGGGCTTGCTCAAGGCCATGCAGGGCATCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

49.735

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

49.91

98.41

0.491
  pilB Vibrio campbellii strain DS40M4

48.07

100

0.484
  pilF Neisseria gonorrhoeae MS11

47.247

99.47

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.163

92.933

0.364
  pilF Thermus thermophilus HB27

36.283

99.823

0.362
  pilB Haemophilus influenzae 86-028NP

42.412

84.982

0.36

Multiple sequence alignment