Detailed information NTG0970753

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: WJM45_RS16760
• Genome accession: NZ_CP150634
• Coordinates: 4006506..4008224 (-)
• Length: 572 a.a.
• NCBI ID: WP_341326203.1
• Uniprot ID: -
• Organism: Methylotuvimicrobium sp. KM2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4001506..4013224

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WJM45_RS16745 (WJM45_16745)	-	4001708..4002607 (+)	900	WP_341326200.1	hypothetical protein	-
WJM45_RS16750 (WJM45_16750)	-	4002692..4004503 (+)	1812	WP_341326201.1	hypothetical protein	-
WJM45_RS16755 (WJM45_16755)	pilC	4005283..4006503 (-)	1221	WP_341326202.1	type II secretion system F family protein	Machinery gene
WJM45_RS16760 (WJM45_16760)	pilB	4006506..4008224 (-)	1719	WP_341326203.1	type IV-A pilus assembly ATPase PilB	Machinery gene
WJM45_RS16765 (WJM45_16765)	-	4008333..4011143 (-)	2811	WP_341326204.1	valine--tRNA ligase	-
WJM45_RS16770 (WJM45_16770)	-	4011209..4011637 (-)	429	WP_341326205.1	DNA polymerase III subunit chi	-
WJM45_RS16775 (WJM45_16775)	-	4011638..4013137 (-)	1500	WP_341326206.1	leucyl aminopeptidase	-

Sequence

Protein

• Length: 572 a.a.
• Molecular weight: 62798.67 Da
• Isoelectric Point: 6.4430

>NTDB_id=970753 WJM45_RS16760 WP_341326203.1 4006506..4008224(-) (pilB) [Methylotuvimicrobium sp. KM2]
MVTDLIDLPIGGLPKCLVQAGLITEADMQLHMQAAKKAKLPLISYLVTNKLVDSKSVTAKASKEFGIPQLDLDALDIRSL
PINLVSEKLIRKHHALPLLKRGNRLFLAVSDPGNFQAQDEIKFHTRLNIDTILVEEDKLSKAIETSLEAADTTMTDLLDA
DLENLEITGGDHESTEDEAASDVDDAPIVRFVNKILLDSIKKGVSDIHLEPYEKSFRIRFRADGMLYEVASPPPNIAGRI
ISRIKVMAKMDIAERRVPQDGRIKMALSKNRAIDFRVNSCPTLFGEKVVLRILDPTSAQIGIEKLGFEPIQQQRFLEAIN
RPYGMVLVTGPTGSGKTVTLYTGLNLLNSVERNISTAEDPVEITVPGINQVNMNPKAGLTFAGALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLVQMGVPPFNIVSAVHLIMAQRLARRLCEYCKVKSDLPDSVLISA
GFKEEHVGKLKIFTANPNGCEHCTSGFKGRVGIYQVMPISENMRKLILEGGNTMQLAAQAKAEGINDLRASGLEKVSQGI
TSLEEIDRVTKE

Nucleotide

• Length: 1719 bp

>NTDB_id=970753 WJM45_RS16760 WP_341326203.1 4006506..4008224(-) (pilB) [Methylotuvimicrobium sp. KM2]
ATGGTAACAGATTTAATCGACCTGCCGATCGGCGGGCTACCGAAATGTTTGGTACAAGCCGGCCTGATTACCGAAGCGGA
TATGCAGTTGCATATGCAGGCAGCGAAGAAAGCTAAATTACCGCTGATTAGTTATTTAGTGACTAACAAGTTAGTGGATA
GCAAATCCGTGACTGCGAAAGCTTCCAAAGAGTTCGGTATTCCGCAGCTTGATCTCGACGCGCTCGATATTCGTAGCTTA
CCTATCAATCTAGTCAGCGAAAAACTGATTAGAAAGCATCATGCTTTACCGTTGCTTAAGCGTGGAAACCGGTTGTTTTT
GGCGGTTTCCGACCCCGGCAATTTTCAAGCTCAGGACGAGATAAAGTTCCATACGCGCCTGAATATCGACACGATTCTGG
TCGAAGAAGATAAATTGTCTAAGGCTATCGAGACATCGTTGGAAGCTGCCGATACCACGATGACCGATTTGCTGGATGCC
GACCTGGAGAATTTGGAAATTACCGGCGGCGATCACGAGTCGACTGAAGACGAGGCTGCGAGCGATGTGGACGATGCGCC
TATTGTTCGTTTCGTCAATAAAATTTTACTCGATTCGATTAAAAAAGGGGTTTCGGACATTCATCTTGAGCCTTATGAAA
AAAGTTTCCGAATCCGCTTTCGGGCGGATGGTATGCTCTATGAAGTGGCAAGTCCGCCTCCCAATATTGCAGGCCGGATT
ATTTCCCGTATCAAGGTCATGGCTAAAATGGATATCGCCGAGCGGCGCGTGCCTCAGGACGGGCGGATTAAGATGGCGCT
GTCGAAAAATCGGGCAATCGACTTTCGGGTCAATAGTTGTCCGACATTGTTCGGCGAAAAAGTCGTACTGCGTATTCTCG
ATCCGACCAGCGCGCAAATCGGCATCGAAAAACTCGGCTTTGAACCCATTCAACAACAACGATTCCTTGAGGCGATCAAC
AGGCCTTACGGCATGGTTTTGGTAACTGGCCCTACCGGTAGCGGTAAAACCGTTACCCTTTACACGGGCTTGAATTTGCT
CAATAGCGTCGAACGGAATATATCGACCGCCGAAGATCCGGTTGAAATTACGGTGCCGGGTATCAATCAGGTCAATATGA
ATCCGAAAGCCGGGTTGACCTTTGCCGGTGCCTTGCGGGCTTTTCTGCGACAAGATCCGGATATCATCATGGTCGGTGAG
ATACGGGATTTGGAAACCGCCGAAATTGCCGTCAAGGCCGCTCAAACTGGCCATTTGGTATTATCCACGCTGCACACTAA
CGATGCGCCGCAAACGTTAAATCGACTCGTGCAAATGGGCGTGCCGCCGTTTAACATCGTTTCGGCGGTGCATTTGATCA
TGGCTCAACGCCTCGCAAGACGTTTATGCGAATATTGTAAGGTAAAATCCGATTTACCGGATTCGGTGTTGATTTCGGCC
GGTTTTAAGGAAGAACATGTAGGAAAATTGAAAATTTTTACGGCCAATCCTAACGGTTGCGAACATTGCACTAGCGGCTT
CAAAGGCCGTGTCGGTATTTATCAGGTTATGCCGATCAGCGAAAATATGCGGAAACTGATTTTGGAAGGCGGCAATACCA
TGCAATTGGCCGCACAAGCCAAAGCCGAAGGCATCAACGACCTGCGCGCGTCGGGTCTTGAGAAAGTCAGTCAAGGCATC
ACTAGCCTGGAAGAAATCGACCGGGTCACTAAGGAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (194-461)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-145)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	56.738	98.601	0.559
pilB	Acinetobacter baylyi ADP1	52.734	99.126	0.523
pilB	Acinetobacter baumannii D1279779	53.203	98.252	0.523
pilB	Vibrio cholerae strain A1552	47.478	100	0.477
pilF	Neisseria gonorrhoeae MS11	48.128	98.077	0.472
pilB	Vibrio parahaemolyticus RIMD 2210633	47.611	98.776	0.47
pilB	Vibrio campbellii strain DS40M4	47.611	98.776	0.47
pilF	Thermus thermophilus HB27	38.246	99.65	0.381
pilB/pilB1	Synechocystis sp. PCC 6803	41.699	90.559	0.378
pilB	Haemophilus influenzae Rd KW20	39.13	92.483	0.362
pilB	Haemophilus influenzae 86-028NP	38.941	92.483	0.36