Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VUK78_RS12200 Genome accession   NZ_CP150467
Coordinates   343918..345675 (+) Length   585 a.a.
NCBI ID   WP_195724146.1    Uniprot ID   -
Organism   Vibrio harveyi strain NH-LM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 338918..350675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK78_RS12180 (VUK78_12180) pilM 341217..342233 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  VUK78_RS12185 (VUK78_12185) pilN 342217..342792 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  VUK78_RS12190 (VUK78_12190) pilO 342785..343378 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  VUK78_RS12195 (VUK78_12195) pilP 343368..343883 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  VUK78_RS12200 (VUK78_12200) pilQ 343918..345675 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  VUK78_RS12205 (VUK78_12205) aroK 345865..346383 (+) 519 WP_005451507.1 shikimate kinase AroK -
  VUK78_RS12210 (VUK78_12210) aroB 346415..347515 (+) 1101 WP_009697563.1 3-dehydroquinate synthase -
  VUK78_RS12215 (VUK78_12215) - 347569..349071 (+) 1503 WP_005451510.1 AAA family ATPase -
  VUK78_RS12220 (VUK78_12220) - 349141..349980 (+) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 63940.93 Da        Isoelectric Point: 4.4829

>NTDB_id=970406 VUK78_RS12200 WP_195724146.1 343918..345675(+) (pilQ) [Vibrio harveyi strain NH-LM1]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGDGEDGGGSVGIDDFLNVNLAATS
PNATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKV
TPQITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGA
LFRRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=970406 VUK78_RS12200 WP_195724146.1 343918..345675(+) (pilQ) [Vibrio harveyi strain NH-LM1]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGGCGTTATC
TAGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATTCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGCGATGGCGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGC
CCTAATGCAACCAGTATTGCTTTCCAAGTTGCAAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCA
ACAAGAATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTA
CAGAAATTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTG
ACACCGCAGATCACGCCTGATAACCGTTTGGTACTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAAC
TGGTACCGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGC
TTGGTGGTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCA
CTTTTCCGTCGAAGTTACGAAAATGTTGGTAAAAGTGAGTTGCTGATTTTTGTTACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(425-583)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

96.752

100

0.968
  pilQ Vibrio cholerae strain A1552

77.058

97.607

0.752
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.058

97.607

0.752
  pilQ Pseudomonas aeruginosa PAK

40.172

99.145

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.123

94.359

0.388
  pilQ Legionella pneumophila strain Lp02

41.123

94.359

0.388
  pilQ Acinetobacter baumannii D1279779

36.877

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.877

100

0.379
  comQ Acinetobacter baylyi ADP1

36.86

100

0.369

Multiple sequence alignment